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Product Info Summary
| SKU: | PA1417 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Thrombospondin 2/THBS2 Antibody Picoband®
SKU/Catalog Number
PA1417
BA3616-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Thrombospondin 2/THBS2 Antibody catalog # PA1417. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Thrombospondin 2/THBS2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1417)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Thrombospondin 2, different from the mouse sequence by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1417 is reactive to THBS2 in Human
Observed Molecular Weight
160 kDa
Calculated molecular weight
130.0 kDa
Background of THBS2
Thrombospondin-2 (THBS2) is a protein that in humans is encoded by the THBS2 gene. The protein encoded by this gene belongs to the thrombospondin family. The THBS2 is mapped to 6q27 and it is located on chromosome 17. The gene was transcribed in fibroblasts, smooth muscle cells, and an osteosarcoma cell line. It functions as a protein inhibitor of tumor growth and angiogenesis and modulates the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1417 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human U87 whole cell, human U251 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of THBS2 using anti-THBS2 antibody (PA1417).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THBS2 antigen affinity purified polyclonal antibody (Catalog # PA1417) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THBS2 at approximately 160 kDa. The expected band size for THBS2 is at 130 kDa.
Specific Publications For Anti-Thrombospondin 2/THBS2 Antibody Picoband® (PA1417)
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7 Customer Q&As for Anti-Thrombospondin 2/THBS2 Antibody Picoband®
Question
Could you tell me the homology between the immunogen and bovine samples?
Verified customer
Asked: 2021-12-07
Answer
The immunogen sequence shares 94.7% homology with bovine Thrombospondin 2. https://www.uniprot.org/blast/uniprot/B202112084ABAA9BC7178C81CEBC9459510EDDEA30238E10?fil=organism:9913
Boster Scientific Support
Answered: 2021-12-08
Question
Do you have information about the homology of PA1417 with human thrombospondin 1?
Verified customer
Asked: 2021-02-17
Answer
Our lab believes that the Anti-Thrombospondin 2/THBS2 Antibody (PA1417) is not likely to cross react with human thrombospondin 1. However we haven't experimentally validated cross reactivity of the antibody with human thrombospondin 1. We can not 100% deny the possibility.
Boster Scientific Support
Answered: 2021-02-23
Question
I see that the anti-Thrombospondin 2/THBS2 antibody PA1417 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-03
Question
Would PA1417 antibody be suitable for 'in vivo' assay?
Verified customer
Asked: 2019-08-21
Answer
The Anti-Thrombospondin 2/THBS2 Antibody PA1417 hasn't been used 'in vivo' assay. It is suggested to validate the antibody and see if it is suitable for it. BSA and preservative (Thimerosal and NaN3) should be removed before running the assay.
Boster Scientific Support
Answered: 2019-08-22
Question
Is a blocking peptide available for product anti-Thrombospondin 2/THBS2 antibody (PA1417)?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
We do provide the blocking peptide for product anti-Thrombospondin 2/THBS2 antibody (PA1417). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-12
Question
We are currently using anti-Thrombospondin 2/THBS2 antibody PA1417 for mouse tissue, and we are happy with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-13
Answer
The anti-Thrombospondin 2/THBS2 antibody (PA1417) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-13
Question
I have a question about product PA1417, anti-Thrombospondin 2/THBS2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Kulkarni
Verified customer
Asked: 2016-03-29
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1417 anti-Thrombospondin 2/THBS2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-03-29
