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Product Info Summary
| SKU: | M02716 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® (monoclonal, 9I6C3)
SKU/Catalog Number
M02716
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® (monoclonal, 9I6C3) catalog # M02716. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® (monoclonal, 9I6C3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02716)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
9I6C3
Isotype
Mouse IgG1
Immunogen
E.coli-derived human Ankyrin erythroid/ANK/ANK1 recombinant protein (Position: N1300-Q1844).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02716 is reactive to ANK1 in Human
Observed Molecular Weight
206 kDa
Calculated molecular weight
206.3 kDa
Background of ANK1
Ankyrin 1, erythrocytic, also known as ANK1, is a protein that in humans is encoded by the ANK1 gene. Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02716 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Positive Control
WB: human HEL whole cell, human K562 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Ankyrin erythroid/ANK/ANK1 using anti-Ankyrin erythroid/ANK/ANK1 antibody (M02716).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ankyrin erythroid/ANK/ANK1 antigen affinity purified monoclonal antibody (Catalog # M02716) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ankyrin erythroid/ANK/ANK1 at approximately 206 kDa. The expected band size for Ankyrin erythroid/ANK/ANK1 is at 206 kDa.
Specific Publications For Anti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® (monoclonal, 9I6C3) (M02716)
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