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Product Info Summary
| SKU: | M01451-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Annexin A1 (ANXA1) Mouse Monoclonal Antibody [Clone ID: OTI3A8]
SKU/Catalog Number
M01451-2
Size
100 µl
Description
Boster Bio Anti-ANXA1 (Annexin A1) mouse monoclonal antibody, clone OTI3A8 (formerly 3A8). Catalog# M01451-2. Tested in IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Annexin A1 (ANXA1) Mouse Monoclonal Antibody [Clone ID: OTI3A8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01451-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3A8
Isotype
IgG1
Immunogen
Full length human recombinant protein of human ANXA1 (NP_000691) produced in HEK293T cell.
Reactive Species
M01451-2 is reactive to ANXA1 in Human, Mouse, Rat
Observed Molecular Weight
38.5 kDa
Calculated molecular weight
38.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01451-2 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC: 1:50
IF: 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANXA1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ANXA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-ANXA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ANXA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Anti-ANXA1 mouse monoclonal antibody (M01451-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA1.
Specific Publications For Anti-Annexin A1 (ANXA1) Mouse Monoclonal Antibody [Clone ID: OTI3A8] (M01451-2)
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