Mouse Fractalkine/CX3CL1 PicoKine™ ELISA Kit
SKU | EK0357 |
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Size | 96wells/kit, with removable strips. |
Reactivity | Mouse |
Sample Type | cell culture supernates, serum and plasma(heparin, EDTA). |
Sensitivity | <10pg/ml |
Assay Range | 312pg/ml-20000pg/ml |
Overview
Product Name | Mouse Fractalkine/CX3CL1 PicoKine™ ELISA Kit |
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SKU/Catalog Number | EK0357 |
Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
Size | 96wells/kit, with removable strips. |
Description | Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Fractalkine/CX3CL1. 96wells/kit, with removable strips. |
Cite This Product | Mouse Fractalkine/CX3CL1 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0357) |
Sample Type | cell culture supernates, serum and plasma(heparin, EDTA)..
Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants thata are not specified above or contact us to check for feasibility. |
Sensitivity | <10pg/ml |
Assay Range | 312pg/ml-20000pg/ml |
Immunogen | Expression system for standard: sf21; Immunogen sequence: Q25-R337 |
Reactivity | Mouse |
Cross Reactivity | There is no detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC. |
Antibody Clonalities | Capture Antibody | Detection Antibody: monoclonal antibody from rat|polyclonal antibody from goat |
Assay Details
Kit Components
Catalog number | Description | Quantity |
EK0357-CAP | 96-well plate precoated with anti-Mouse CX3CL1 antibody | 1 |
EK0357-ST | lyophilized recombinant Mouse CX3CL1 standard | 20ng/tube |
EK0357-DA | biotinylated anti-Mouse CX3CL1 antibody | 130ul |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
AR1106-1 | sample diluent buffer | 30ml |
AR1106-2 | antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Mouse Fractalkine/CX3CL1 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 20-25min)
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.094 | 0.110 | 0.156 | 0.253 | 0.566 | 0.924 | 1.437 | 1.982 |
Intra/Inter Assay Precision
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 378 | 2168 | 9077 | 392 | 1975 | 9206 |
Standard deviation | 17.76 | 101.89 | 653.54 | 23.52 | 114.55 | 810.12 |
CV(%) | 4.7% | 4.7% | 7.2% | 6% | 5.8% | 8.8% |
Reproducibility
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 378 | 415 | 410 | 441 | 411 | 22.39 | 5.4% |
Sample 2 | 2168 | 2082 | 1886 | 2198 | 2083 | 121.71 | 5.8% |
Sample 3 | 9077 | 9811 | 9555 | 9593 | 9509 | 267.86 | 2.8% |
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Target Info
Protein Target Info (Source: Uniprot.org)
Uniprot Id | A0N0N7 |
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Gene Name | CX3CL1 |
Protein Name | chemokine (C-X3-C motif) ligand 1 |
Alternative Names | Chemokine (C-X3-C motif) ligand 1 ;cDNA, FLJ94424, Homo sapiens chemokine (C-X3-C motif) ligand 1 (CX3CL1), mRNA ;CX3CL1 ;hCG_15105 ; |
Molecular Weight | 42203 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
Research Areas | Atherosclerosis, Cardiovascular, Chemokines, Immunology, Innate Immunity, Macrophage / Inflamm., Vascular Inflammation *You can search these to find other products in these research areas. |
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Background | Chemokine(C-X3-C motif) ligand 1(CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine(in humans) and neurotactin(in mice). CX3CL1 is produced as a long protein(with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble(90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment. |
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Customer Q&As
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
• If no signal is produced, then you can work on the tissue sample by using the kit.
The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
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