|Product Name||Anti-Annexin IV/ANXA4 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Annexin A4(ANXA4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-Annexin IV/ANXA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1007-1)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin IV(7-21aa TVKAASGFNAMEDAQ), different from the related mouse and rat sequences by one amino acid.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-Annexin IV antibody, PA1007-1, Western blotting
Lane 1: Human Placenta Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: 293T Cell Lysate
Lane 4: HEPA Cell Lysate
Lane 5: NIH3T3 Cell Lysate
Anti-Annexin IV antibody, PA1007-1, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-Annexin IV antibody, PA1007-1, IHC(P)
IHC(P): Rat Pancreas Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Annexin A4|
|Alternative Names||Annexin A4;35-beta calcimedin;Annexin IV;Annexin-4;Carbohydrate-binding protein p33/p41;Chromobindin-4;Endonexin I;Lipocortin IV;P32.5;PP4-X;Placental anticoagulant protein II;PAP-II;Protein II;ANXA4;ANX4;|
|Molecular Weight||35883 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. .|
|Research Areas||Calcium Binding Proteins, Calcium Signaling, Cardiovascular, Cell Adhesion, Cytoskeleton / Ecm, Signal Transduction, Signaling Pathway
*You can search these to find other products in these research areas.
|Background||Annexin IV, also known as placental anticoagulant protein II, or zymogen granule membrane-associated protein, 36-KD(ZAP36), is one of the family of calcium-dependent phospholipid-binding proteins whose normal function is uncertain. Annexin IV has 45 to 59% identity with other members of the annexin family. Annexin IV gene spans 18 to 56 kb. And it is located on mouse chromosome 6 in a region of conserved synteny with human chromosome 2. Rat annexin IV is ubiquitously expressed in epithelial tissues. Zap36 associated with zymogen granule membranes in dog and rat pancreas. Human annexin IV gene is mapped to chromosome 2p13.|
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (PA1007-1)
Promotion:Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,