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- Table of Contents
ANXA4 antibody (PA1007-1) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of Annexin IV/ANXA4 using anti-Annexin IV/ANXA4 antibody (PA1007-1).
Annexin IV/ANXA4 was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Annexin IV/ANXA4 Antibody (PA1007-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Annexin IV/ANXA4 was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Annexin IV/ANXA4 Antibody (PA1007-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Annexin IV/ANXA4 Antibody Picoband®
ANXA4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
ANXA4 antibody was positively detected by WB in:
human Caco-2 whole cell, human SiHa whole cell, human Hacat whole cell, human Hela whole cell, human U251 whole cell, human A549 whole cell, human PC-3 whole cell, rat lung tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ANXA4 antibody was positively detected by IHC in:
Human Mammary Cancer tissue, Rat Pancreas tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat, Mouse
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Bovine
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Annexin IV/ANXA4 Antibody Picoband®
View all ANXA4 Antibodies
View all ANXA4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin IV, different from the related mouse and rat sequences by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated Molecular Weight
35.9 kDa
Background of ANXA4
Annexin IV, also known as placental anticoagulant protein II, or zymogen granule membrane-associated protein, 36-KD (ZAP36), is one of the family of calcium-dependent phospholipid-binding proteins whose normal function is uncertain. Annexin IV has 45 to 59% identity with other members of the annexin family. Annexin IV gene spans 18 to 56 kb. And it is located on mouse chromosome 6 in a region of conserved synteny with human chromosome 2. Rat annexin IV is ubiquitously expressed in epithelial tissues. Zap36 associated with zymogen granule membranes in dog and rat pancreas. Human annexin IV gene is mapped to chromosome 2p13.
Cite This Product
Anti-Annexin IV/ANXA4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1007-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
93%
High Homology
Rat
93%
High Homology
Cow
93%
High Homology
Monkey
93%
High Homology
Rabbit
87%
Moderate Homology
Sheep
87%
Moderate Homology
Dog
80%
Moderate Homology
Zebrafish
60%
Low homology
Drosophila
60%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ANXA4 antibody
Immunohistochemistry (IHC) protocol for ANXA4 antibody
Publications
Application:
Species:
Trafficking of Annexins during Membrane Repair in Human Skeletal Muscle Cells
Published: 2022-01-26
Journal: Membranes
Application: WB
Impact Factor: 4.56
Species: Human
PMID: 35207075
Annexin A1, A2, A4 and A5 play important roles in breast cancer, pancreatic cancer and laryngeal carcinoma,alone and/or synergistically
Published: 2012-10-09
Journal: Oncology Letters
Application: IHC
Impact Factor: 0.11
Species: Human
PMID: 23255903
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Product Promise
We promise Anti-Annexin IV/ANXA4 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1007-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026