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Product Info Summary
| SKU: | PA1007-1 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Annexin IV/ANXA4 Antibody
View all Annexin A4 Antibodies
SKU/Catalog Number
PA1007-1
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Annexin IV/ANXA4 Antibody catalog # PA1007-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Annexin IV/ANXA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1007-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin IV (7-21aa TVKAASGFNAMEDAQ), different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross Reactivity
No cross reactivity with other proteins
Reactive Species
PA1007-1 is reactive to ANXA4 in Human, Mouse, Rat
Applications
PA1007-1 is guaranteed for IHC, WB Boster Guarantee
Background of Annexin A4
Annexin IV, also known as placental anticoagulant protein II, or zymogen granule membrane-associated protein, 36-KD (ZAP36), is one of the family of calcium-dependent phospholipid-binding proteins whose normal function is uncertain. Annexin IV has 45 to 59% identity with other members of the annexin family. Annexin IV gene spans 18 to 56 kb. And it is located on mouse chromosome 6 in a region of conserved synteny with human chromosome 2. Rat annexin IV is ubiquitously expressed in epithelial tissues. Zap36 associated with zymogen granule membranes in dog and rat pancreas. Human annexin IV gene is mapped to chromosome 2p13.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Anti-Annexin IV antibody, PA1007-1, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-Annexin IV antibody, PA1007-1, IHC(P)
IHC(P): Rat Pancreas Tissue
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Anti-Annexin IV antibody, PA1007-1, Western blotting
Lane 1: Human Placenta Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: 293T Cell Lysate
Lane 4: HEPA Cell Lysate
Lane 5: NIH3T3 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For ANXA4 (Source: Uniprot.Org, NCBI)
Uniprot ID
P09525
Gene ID
307
Gene Name
ANXA4
Full Name
Annexin A4
Weight
35.883kDa
Superfamily
annexin family
Alternative Names
AIV; Annexin A4; annexin IV (placental anticoagulant protein II); Annexin IV; annexin-4; ANX4; ANX4Carbohydrate-binding protein p33/p41; ANXA4; Chromobindin-4; chromobindin-4,35-beta calcimedin; DKFZp686H02120; Endonexin I; Lipocortin IV; MGC75105; P32.5; PAP-II; PIG28; Placental anticoagulant protein II; PP4-X; proliferation-inducing gene 28; proliferation-inducing protein 28; Protein II; ZAP36 ANXA4 ANX4, HEL-S-274, P32.5, PAP-II, PIG28, PP4-X, ZAP36 annexin A4 annexin A4|35-beta calcimedin|annexin IV (placental anticoagulant protein II)|annexin-4|carbohydrate-binding protein p33/p41|chromobindin-4|endonexin I|epididymis secretory protein Li 274|lipocortin IV|placental anticoagulant protein II|proliferation-inducing gene 28|proliferation-inducing protein 28|protein II
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ANXA4, check out the ANXA4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ANXA4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-Annexin IV/ANXA4 Antibody (PA1007-1)
PA1007-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Annexin A1, A2, A4 and A5 play important roles in breast cancer, pancreatic cancer and laryngeal carcinoma,alone and/or synergistically
Annexin A1, A2, A4 and A5 play important roles in breast cancer, pancreatic cancer and laryngeal carcinoma, alone and/or synergistically
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Annexin IV/ANXA4 Antibody
Question
Does anti-Annexin IV/ANXA4 antibody PA1007-1 work on horse IHC with adrenal gland?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Our lab technicians have not tested anti-Annexin IV/ANXA4 antibody PA1007-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Annexin IV/ANXA4 antibody PA1007-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse adrenal gland in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
Does PA1007-1 anti-Annexin IV/ANXA4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can see on the product datasheet, PA1007-1 anti-Annexin IV/ANXA4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-03
Question
We are currently using anti-Annexin IV/ANXA4 antibody PA1007-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
The anti-Annexin IV/ANXA4 antibody (PA1007-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-27
Question
Is a blocking peptide available for product anti-Annexin IV/ANXA4 antibody (PA1007-1)?
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
We do provide the blocking peptide for product anti-Annexin IV/ANXA4 antibody (PA1007-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-25
Question
Is this PA1007-1 anti-Annexin IV/ANXA4 antibody reactive to the isotypes of ANXA4?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
The immunogen of PA1007-1 anti-Annexin IV/ANXA4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin IV(7-21aa TVKAASGFNAMEDAQ), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-13
Question
Will anti-Annexin IV/ANXA4 antibody PA1007-1 work for WB with liver?
W. Jones
Verified customer
Asked: 2015-08-14
Answer
According to the expression profile of liver, ANXA4 is highly expressed in liver. So, it is likely that anti-Annexin IV/ANXA4 antibody PA1007-1 will work for WB with liver.
Boster Scientific Support
Answered: 2015-08-14
