Anti-CYP7A1 Antibody Picoband™

CYP7A1 antibody

Boster Bio Anti-CYP7A1 Antibody Picoband™ catalog # A01601. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: A01601
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-CYP7A1 Antibody Picoband™

View all CYP7A1 Antibodies

SKU/Catalog Number

A01601

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CYP7A1 Antibody Picoband™ catalog # A01601. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CYP7A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01601)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CYP7A1 recombinant protein (Position: I10-L504).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01601 is reactive to CYP7A1 in Human

Applications

A01601 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

58 kDa

Calculated molecular weight

57.661kDa

Background of CYP7A1

CYP7A1 (Cytochrome P450 Subfamily VIIA Polypeptide 1), also called CYP7 or CHOLESTEROL 7-ALPHA-HYDROXYLASE, is an enzyme that in humans is encoded by the CYP7A1 gene. Using both mouse-human somatic cell hybrids and in situ chromosomal hybridization, Cohen et al. (1992) mapped the CYP7 gene to 8q11-q12. By transfection of reporter constructs, mutation analysis, and DNase footprinting, Molowa et al. (1992) identified areas of the CYP7A1 promoter region that showed hepatocyte-specific activation. They found HNF3 to be an activator of CYP7A1 activity. Agellon et al. (2002) found that wildtype mice and mice transgenic for human CYP7A1 respond differently to cholesterol feeding. Cholesterol feeding stimulated Cyp7a1 mRNA abundance and enzymatic activity in wildtype mice, but repressed human CYP7A1 mRNA and activity in transgenic mice.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section, 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CYP7A1 (Source: Uniprot.org, NCBI)

Gene Name

CYP7A1

Full Name

Cytochrome P450 7A1

Weight

57.661kDa

Superfamily

cytochrome P450 family

Alternative Names

Cholesterol 7-alpha-hydroxylase; cholesterol 7-alpha-monooxygenase; CP7A; CYP7; CYP7A1; CYP7MGC126826; CYPVII; Cytochrome P450 7A1; cytochrome P450, family 7, subfamily A, polypeptide 1; cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase); cytochrome P450, subfamily VIIA polypeptide 1; EC 1.14.13; EC 1.14.13.17; MGC138389; polypeptide 1 CYP7A1 CP7A, CYP7, CYPVII cytochrome P450 family 7 subfamily A member 1 cytochrome P450 7A1|24-hydroxycholesterol 7-alpha-hydroxylase|cholesterol 7-alpha-hydroxylase|cholesterol 7-alpha-monooxygenase|cholesterol 7alpha-hydroxylase|cytochrome P450, family 7, subfamily A, polypeptide 1|cytochrome P450, subfamily VIIA polypeptide 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CYP7A1, check out the CYP7A1 Infographic

CYP7A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CYP7A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01601 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cellular Accumulation and Toxic Effects of Bile Acids in Cyclosporine A-Treated HepaRG Hepatocytes

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Order DetailsPrice
A01601

100μg

$370
A01601-10ug

10μg sample (liquid)

$99
A01601-Biotin

100 μg Biotin conjugated

$570
A01601-Cy3

100 μg Cy3 conjugated

$570
A01601-Dylight488

100 μg Dylight488 conjugated

$570
A01601-Dylight550

100 μg Dylight550 conjugated

$570
A01601-Dylight594

100 μg Dylight594 conjugated

$570
A01601-FITC

100 μg FITC conjugated

$570
A01601-HRP

100 μg HRP conjugated

$570
A01601-APC

100 μg APC conjugated

$670
A01601-PE

100 μg PE conjugated

$670
A01601-carrier-free

Carrier Free

$370

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A01601
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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