Anti-Estrogen Inducible Protein pS2/TFF1 Antibody
Rabbit IgG polyclonal antibody for Trefoil factor 1(TFF1) detection. Tested with WB, IHC-P in Human.
Anti-Estrogen Inducible Protein pS2/TFF1 Antibody Info At A Glance
|Product Name||Anti-Estrogen Inducible Protein pS2/TFF1 Antibody
See all TFF1/pS2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for PS2/TFF1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. PS2/TFF1 information: Molecular Weight: 9150 MW; Subcellular Localization: Secreted ; Tissue Specificity: Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level). Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level). Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level).|
|Cite This Product||Anti-Estrogen Inducible Protein pS2/TFF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1689)|
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|Anti-Estrogen Inducible Protein pS2/TFF1 Antibody may replace the following items: sc 22501|sc 22502|sc 271464|sc 28925|sc 390889|sc 7842.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Estrogen Inducible Protein pS2(67-84aa WCFYPNTIDVPPEEECEF).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For TFF1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||7031|
|Species Of This Entry||Human|
|Protein Name||Trefoil factor 1|
|Alternative Names||TFF1/pS2|BCEI; BCEIbreast cancer, estrogen-inducible sequence expressed in; Breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; D21S21; gastrointestinal trefoil protein pS2; hP1.A; HPS2; PNR-2; Polypeptide P1.A; Protein pS2; PS2; TFF1; trefoil factor 1; trefoil factor, BCE1, human pS2 induced by estrogen from human breast cancercell line M10HP1.A|
|Post Tranlational Modifications||Methylation; Phosphorylation; Acetylation; Demethylation; Cleavage; Glycosylation; Bromination; Biotinylation; Phosphorothioation; Hydroxylation; Deacetylation; Gpi Anchoring|
|Gene Location||21q22.3, on Chromasome 21, gene sequence: NC_000021.9 (42362282..42366535, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For TFF1 (Source: Uniprot.org, NCBI)
|Protein Function||Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.|
|Tissue Specificity||Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level). Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level). Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level).|
|Interacting Proteins||BTN3A1; BTNL8; CD300LD; FCRL4; SIGLEC8; UBQLN1|
|Research Areas||Breast Cancer; Cancer|
|Related Diseases||Malignant Neoplasms; Mammary Neoplasms; Malignant Neoplasm Of Breast; Neoplasms; Carcinoma; Stomach Neoplasms; Malignant Neoplasm Of Stomach; Adenocarcinoma; Neoplasm Metastasis; Hyperplasia; Ulcer; Mucinous Adenocarcinoma; Ductal Breast Carcinoma; Malignant Paraganglionic Neoplasm; Lymphatic Metastasis; Neoplasms, Hormone-dependent; Cell Invasion; Cell Transformation, Neoplastic; Inflammation; Metaplasia|
|Related Pathways||Cell Proliferation; Localization; Cell Growth; Methylation; Cell Cycle; Secretion; Pathogenesis; Cell Differentiation; Dna Methylation; Cell Migration; Wound Healing; Reverse Transcription; Angiogenesis; Cell Motility; Chromatin Remodeling; Regeneration; Demethylation; Cell Death; Menopause; Acid Secretion|
|Background||TFF1(Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.|
|Scientific References||PMID: 6324130 by Jakowlew S.B., et al. Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7.|
PMID: 3838275 by Prud'Homme J.-F., et al. Cloning of a gene expressed in human breast cancer and regulated by estrogen in MCF-7 cells.
Our Boster Quality Guarantee for Anti-Estrogen Inducible Protein pS2/TFF1 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
IHC(P): Human Gastric Cancer Tissue
WB: MCF-7 Cell Lysate
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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