Product Info Summary
SKU: | PA2303 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Mucin 5AC/MUC5AC Antibody
SKU/Catalog Number
PA2303
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mucin 5AC/MUC5AC Antibody catalog # PA2303. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mucin 5AC/MUC5AC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2303)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Mucin 5AC.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2303 is reactive to MUC5AC in Human
Applications
PA2303 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
300 kDa
Calculated molecular weight
585.57kDa
Background of MUC5AC
Mucin-5AC is a protein that in humans is encoded by the MUC5AC gene. It is mapped to 11p15.5. MUC5AC is highly expressed in adult trachea and stomach. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease (COPD). It has been showed that activation of the NF-kappa-B signaling pathway by inflammatory cytokine TNF-alpha upregulated MUC5AC mRNA expression nearly 6-fold. MUC5AC is also a direct and critical mediator of resistance during intestinal nematode infection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Validation Images & Assay Conditions
Click image to see more details
Anti-Mucin 5AC antibody, PA2303, All Western blotting
All lanes: Anti-MUC5AC(PA2303) at0.5ug/ml
Lane 1: SGC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: PANC Whole Cell Lysate at 40ug
Predicted bind size: 527KD
Observed bind size: 300KD
Click image to see more details
Figure 2. IHC analysis of Mucin 5AC using anti-Mucin 5AC antibody (PA2303).
Mucin 5AC was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Mucin 5AC Antibody (PA2303) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MUC5AC (Source: Uniprot.org, NCBI)
Gene Name
MUC5AC
Full Name
Mucin-5AC
Weight
585.57kDa
Alternative Names
gastric mucin; leB; lewis B blood group antigen; major airway glycoprotein; MUC5; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin 5AC, oligomeric mucus/gel-forming pseudogene; mucin 5AC, oligomeric mucus/gel-forming; mucin-5 subtype AC, tracheobronchial; mucin-5AC; TBM; tracheobronchial mucin MUC5AC MUC5, TBM, leB, mucin mucin 5AC, oligomeric mucus/gel-forming mucin-5AC|gastric mucin|lewis B blood group antigen|major airway glycoprotein|mucin 5, subtypes A and C, tracheobronchial/gastric|mucin 5AC, oligomeric mucus/gel-forming pseudogene|mucin-5 subtype AC, tracheobronchial|tracheobronchial mucin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MUC5AC, check out the MUC5AC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MUC5AC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mucin 5AC/MUC5AC Antibody (PA2303)
Hello CJ!
PA2303 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Huang J,Liu J,Chang G,Wang Y,Ma N,Roy AC,Shen X.Glutamine Supplementation Attenuates the Inflammation Caused by LPS-Induced Acute Lung Injury in Mice by Regulating the TLR4/MAPK Signaling Pathway.Inflammation.2021 Jun 23.doi:10.1007/s10753-021-01491-2.Epub ahead of print.PMID:34160729.
Species: Mouse
PA2303 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:1000
Zhou J, Perelman Jm, Kolosov Vp, Zhou X. Mol Cell Biochem. 2013 May;377(1-2):75-85. Doi: 10.1007/S11010-013-1572-3. Epub 2013 Feb 8. Neutrophil Elastase Induces Muc5Ac Secretion Via Protease-Activated Receptor 2.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
16 Customer Q&As for Anti-Mucin 5AC/MUC5AC Antibody
Question
Do you have a BSA free version of anti-Mucin 5AC/MUC5AC antibody PA2303 available?
F. Gonzalez
Verified customer
Asked: 2020-03-04
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Mucin 5AC/MUC5AC antibody PA2303 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-04
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for nasal polyp using anti-Mucin 5AC/MUC5AC antibody PA2303. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-31
Question
My boss were content with the WB result of your anti-Mucin 5AC/MUC5AC antibody. However we have been able to see positive staining in placenta trachea secreted. using this antibody. Is that expected? Could you tell me where is MUC5AC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
From literature, placenta trachea does express MUC5AC. Generally MUC5AC expresses in secreted. Regarding which tissues have MUC5AC expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Nasal polyp, Pubmed ID: 7513696, 7775418
Placenta, and Trachea, Pubmed ID: 9620876
Stomach, Pubmed ID: 17974005
Trachea, Pubmed ID: 9506983
Tracheobronchial mucosa, Pubmed ID: 2656675
Boster Scientific Support
Answered: 2019-12-19
Question
I see that the anti-Mucin 5AC/MUC5AC antibody PA2303 works with IHC-P, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-11
Question
Is a blocking peptide available for product anti-Mucin 5AC/MUC5AC antibody (PA2303)?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
We do provide the blocking peptide for product anti-Mucin 5AC/MUC5AC antibody (PA2303). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-27
Question
I would like using your anti-Mucin 5AC/MUC5AC antibody for dectin-2 family studies. Has this antibody been tested with western blotting on colo320 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
We appreciate your inquiry. This PA2303 anti-Mucin 5AC/MUC5AC antibody is validated on sgc whole cell lysate, hela whole cell lysate, colo320 whole cell lysate, sw620 whole cell lysate, panc whole cell lysate, gastric cancer tissue. It is guaranteed to work for IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-09
Question
Will PA2303 anti-Mucin 5AC/MUC5AC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
It shows on the product datasheet, PA2303 anti-Mucin 5AC/MUC5AC antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-08
Question
Would anti-Mucin 5AC/MUC5AC antibody PA2303 work for IHC-P with nasal polyp?
L. Li
Verified customer
Asked: 2019-07-19
Answer
According to the expression profile of nasal polyp, MUC5AC is highly expressed in nasal polyp. So, it is likely that anti-Mucin 5AC/MUC5AC antibody PA2303 will work for IHC-P with nasal polyp.
Boster Scientific Support
Answered: 2019-07-19
Question
We purchased anti-Mucin 5AC/MUC5AC antibody for WB on placenta trachea last year. I am using human, and We want to use the antibody for IHC-P next. Our lab want to know about examining placenta trachea as well as stomach in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
I looked at the website and datasheets of our anti-Mucin 5AC/MUC5AC antibody and it seems that PA2303 has been tested on human in both WB and IHC-P. Thus PA2303 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-24
Question
We have seen staining in human trachea. Any tips? Is anti-Mucin 5AC/MUC5AC antibody supposed to stain trachea positively?
Verified Customer
Verified customer
Asked: 2019-04-03
Answer
From what I have seen in literature trachea does express MUC5AC. From what I have seen in Uniprot.org, MUC5AC is expressed in nasal cavity epithelium, trachea, tracheobronchial mucosa, placenta trachea, nasal polyp, colon, stomach, among other tissues. Regarding which tissues have MUC5AC expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Nasal polyp, Pubmed ID: 7513696, 7775418
Placenta, and Trachea, Pubmed ID: 9620876
Stomach, Pubmed ID: 17974005
Trachea, Pubmed ID: 9506983
Tracheobronchial mucosa, Pubmed ID: 2656675
Boster Scientific Support
Answered: 2019-04-03
Question
Is this PA2303 anti-Mucin 5AC/MUC5AC antibody reactive to the isotypes of MUC5AC?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
The immunogen of PA2303 anti-Mucin 5AC/MUC5AC antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Mucin 5AC(552-567aa TMQLFMQLAPKLRGQT). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-06
Question
We are currently using anti-Mucin 5AC/MUC5AC antibody PA2303 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
The anti-Mucin 5AC/MUC5AC antibody (PA2303) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-30
Question
We want to test anti-Mucin 5AC/MUC5AC antibody PA2303 on human nasal polyp for research purposes, then I may be interested in using anti-Mucin 5AC/MUC5AC antibody PA2303 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-23
Answer
The products we sell, including anti-Mucin 5AC/MUC5AC antibody PA2303, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-23
Question
Please see the WB image, lot number and protocol we used for nasal polyp using anti-Mucin 5AC/MUC5AC antibody PA2303. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-22
Question
I was wanting to use your anti-Mucin 5AC/MUC5AC antibody for IHC-P for human nasal polyp on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human nasal polyp identification?
C. Zhao
Verified customer
Asked: 2015-03-24
Answer
You can see on the product datasheet, PA2303 anti-Mucin 5AC/MUC5AC antibody has been tested for IHC-P, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human nasal polyp in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-24
Question
Can you help my question with product PA2303, anti-Mucin 5AC/MUC5AC antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
V. Patel
Verified customer
Asked: 2014-06-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2303 anti-Mucin 5AC/MUC5AC antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-06-13