Anti-Mucin 5AC/MUC5AC Antibody

MUC5AC antibody

Boster Bio Anti-Mucin 5AC/MUC5AC Antibody catalog # PA2303. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: PA2303
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Mucin 5AC/MUC5AC Antibody

View all MUC5AC Antibodies

SKU/Catalog Number

PA2303

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Mucin 5AC/MUC5AC Antibody catalog # PA2303. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mucin 5AC/MUC5AC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2303)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Mucin 5AC.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2303 is reactive to MUC5AC in Human

Applications

PA2303 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

300 kDa

Calculated molecular weight

585.57kDa

Background of MUC5AC

Mucin-5AC is a protein that in humans is encoded by the MUC5AC gene. It is mapped to 11p15.5. MUC5AC is highly expressed in adult trachea and stomach. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease (COPD). It has been showed that activation of the NF-kappa-B signaling pathway by inflammatory cytokine TNF-alpha upregulated MUC5AC mRNA expression nearly 6-fold. MUC5AC is also a direct and critical mediator of resistance during intestinal nematode infection.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For MUC5AC (Source: Uniprot.org, NCBI)

Gene Name

MUC5AC

Full Name

Mucin-5AC

Weight

585.57kDa

Alternative Names

gastric mucin; leB; lewis B blood group antigen; major airway glycoprotein; MUC5; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin 5AC, oligomeric mucus/gel-forming pseudogene; mucin 5AC, oligomeric mucus/gel-forming; mucin-5 subtype AC, tracheobronchial; mucin-5AC; TBM; tracheobronchial mucin MUC5AC MUC5, TBM, leB, mucin mucin 5AC, oligomeric mucus/gel-forming mucin-5AC|gastric mucin|lewis B blood group antigen|major airway glycoprotein|mucin 5, subtypes A and C, tracheobronchial/gastric|mucin 5AC, oligomeric mucus/gel-forming pseudogene|mucin-5 subtype AC, tracheobronchial|tracheobronchial mucin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MUC5AC, check out the MUC5AC Infographic

MUC5AC infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MUC5AC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA2303 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Huang J,Liu J,Chang G,Wang Y,Ma N,Roy AC,Shen X.Glutamine Supplementation Attenuates the Inflammation Caused by LPS-Induced Acute Lung Injury in Mice by Regulating the TLR4/MAPK Signaling Pathway.Inflammation.2021 Jun 23.doi:10.1007/s10753-021-01491-2.Epub ahead of print.PMID:34160729.
Species: Mouse
PA2303 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:1000

Zhou J, Perelman Jm, Kolosov Vp, Zhou X. Mol Cell Biochem. 2013 May;377(1-2):75-85. Doi: 10.1007/S11010-013-1572-3. Epub 2013 Feb 8. Neutrophil Elastase Induces Muc5Ac Secretion Via Protease-Activated Receptor 2.

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16 Customer Q&As for Anti-Mucin 5AC/MUC5AC Antibody

Question

Do you have a BSA free version of anti-Mucin 5AC/MUC5AC antibody PA2303 available?

F. Gonzalez

Verified customer

Asked: 2020-03-04

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Mucin 5AC/MUC5AC antibody PA2303 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-04

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for nasal polyp using anti-Mucin 5AC/MUC5AC antibody PA2303. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-31

Question

My boss were content with the WB result of your anti-Mucin 5AC/MUC5AC antibody. However we have been able to see positive staining in placenta trachea secreted. using this antibody. Is that expected? Could you tell me where is MUC5AC supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

From literature, placenta trachea does express MUC5AC. Generally MUC5AC expresses in secreted. Regarding which tissues have MUC5AC expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Nasal polyp, Pubmed ID: 7513696, 7775418
Placenta, and Trachea, Pubmed ID: 9620876
Stomach, Pubmed ID: 17974005
Trachea, Pubmed ID: 9506983
Tracheobronchial mucosa, Pubmed ID: 2656675

Boster Scientific Support

Answered: 2019-12-19

Question

I see that the anti-Mucin 5AC/MUC5AC antibody PA2303 works with IHC-P, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-11

Question

Is a blocking peptide available for product anti-Mucin 5AC/MUC5AC antibody (PA2303)?

Verified Customer

Verified customer

Asked: 2019-11-27

Answer

We do provide the blocking peptide for product anti-Mucin 5AC/MUC5AC antibody (PA2303). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-27

Question

I would like using your anti-Mucin 5AC/MUC5AC antibody for dectin-2 family studies. Has this antibody been tested with western blotting on colo320 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

We appreciate your inquiry. This PA2303 anti-Mucin 5AC/MUC5AC antibody is validated on sgc whole cell lysate, hela whole cell lysate, colo320 whole cell lysate, sw620 whole cell lysate, panc whole cell lysate, gastric cancer tissue. It is guaranteed to work for IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-09

Question

Will PA2303 anti-Mucin 5AC/MUC5AC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

It shows on the product datasheet, PA2303 anti-Mucin 5AC/MUC5AC antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-08

Question

Would anti-Mucin 5AC/MUC5AC antibody PA2303 work for IHC-P with nasal polyp?

L. Li

Verified customer

Asked: 2019-07-19

Answer

According to the expression profile of nasal polyp, MUC5AC is highly expressed in nasal polyp. So, it is likely that anti-Mucin 5AC/MUC5AC antibody PA2303 will work for IHC-P with nasal polyp.

Boster Scientific Support

Answered: 2019-07-19

Question

We purchased anti-Mucin 5AC/MUC5AC antibody for WB on placenta trachea last year. I am using human, and We want to use the antibody for IHC-P next. Our lab want to know about examining placenta trachea as well as stomach in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

I looked at the website and datasheets of our anti-Mucin 5AC/MUC5AC antibody and it seems that PA2303 has been tested on human in both WB and IHC-P. Thus PA2303 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-05-24

Question

We have seen staining in human trachea. Any tips? Is anti-Mucin 5AC/MUC5AC antibody supposed to stain trachea positively?

Verified Customer

Verified customer

Asked: 2019-04-03

Answer

From what I have seen in literature trachea does express MUC5AC. From what I have seen in Uniprot.org, MUC5AC is expressed in nasal cavity epithelium, trachea, tracheobronchial mucosa, placenta trachea, nasal polyp, colon, stomach, among other tissues. Regarding which tissues have MUC5AC expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Nasal polyp, Pubmed ID: 7513696, 7775418
Placenta, and Trachea, Pubmed ID: 9620876
Stomach, Pubmed ID: 17974005
Trachea, Pubmed ID: 9506983
Tracheobronchial mucosa, Pubmed ID: 2656675

Boster Scientific Support

Answered: 2019-04-03

Question

Is this PA2303 anti-Mucin 5AC/MUC5AC antibody reactive to the isotypes of MUC5AC?

Verified Customer

Verified customer

Asked: 2019-03-06

Answer

The immunogen of PA2303 anti-Mucin 5AC/MUC5AC antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Mucin 5AC(552-567aa TMQLFMQLAPKLRGQT). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-03-06

Question

We are currently using anti-Mucin 5AC/MUC5AC antibody PA2303 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2018-08-30

Answer

The anti-Mucin 5AC/MUC5AC antibody (PA2303) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-08-30

Question

We want to test anti-Mucin 5AC/MUC5AC antibody PA2303 on human nasal polyp for research purposes, then I may be interested in using anti-Mucin 5AC/MUC5AC antibody PA2303 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-02-23

Answer

The products we sell, including anti-Mucin 5AC/MUC5AC antibody PA2303, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-02-23

Question

Please see the WB image, lot number and protocol we used for nasal polyp using anti-Mucin 5AC/MUC5AC antibody PA2303. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-05-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-22

Question

I was wanting to use your anti-Mucin 5AC/MUC5AC antibody for IHC-P for human nasal polyp on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human nasal polyp identification?

C. Zhao

Verified customer

Asked: 2015-03-24

Answer

You can see on the product datasheet, PA2303 anti-Mucin 5AC/MUC5AC antibody has been tested for IHC-P, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human nasal polyp in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-03-24

Question

Can you help my question with product PA2303, anti-Mucin 5AC/MUC5AC antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

V. Patel

Verified customer

Asked: 2014-06-13

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2303 anti-Mucin 5AC/MUC5AC antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-06-13

Order DetailsPrice
PA2303

100μg

$370
PA2303-10ug

10μg sample (liquid)

$99
PA2303-Biotin

100 μg Biotin conjugated

$570
PA2303-Cy3

100 μg Cy3 conjugated

$570
PA2303-Dylight488

100 μg Dylight488 conjugated

$570
PA2303-Dylight550

100 μg Dylight550 conjugated

$570
PA2303-Dylight594

100 μg Dylight594 conjugated

$570
PA2303-FITC

100 μg FITC conjugated

$570
PA2303-HRP

100 μg HRP conjugated

$570
PA2303-APC

100 μg APC conjugated

$670
PA2303-PE

100 μg PE conjugated

$670
PA2303-carrier-free

Carrier Free

$370

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