Anti-AIM2 Antibody Picoband™

AIM2 antibody

Boster Bio Anti-AIM2 Antibody Picoband™ catalog # PB9683. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PB9683
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-AIM2 Antibody Picoband™

View all AIM2 Antibodies

SKU/Catalog Number

PB9683

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AIM2 Antibody Picoband™ catalog # PB9683. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AIM2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9683)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human AIM2 recombinant protein (Position: L14-H215). Human AIM2 shares 53.8% amino acid (aa) sequence identity with mouse AIM2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9683 is reactive to AIM2 in Human, Mouse, Rat

Applications

PB9683 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

38.954kDa

Background of AIM2

Interferon-inducible protein AIM2, also known as absent in melanoma 2 or simply AIM2, is a protein that in humans is encoded by the AIM2 gene. It is mapped to 1q22. AIM2 is a member of the Ifi202/IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome. The C-terminal HIN domain binds double stranded DNA  (either viral, bacterial, or even host) and acts as a cytosolic dsDNA sensor. This leads to the oligomerization of the inflammasome complex. The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another protein ASC  (or Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain). ASC also contains a CARD domain  (caspase activation and recruitment domain), that recruits procaspase-1 to the complex. This leads to the autoactivation of caspase-1, an enzyme that processes proinflammatory cytokines  (IL-1b and IL-18).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For AIM2 (Source: Uniprot.org, NCBI)

Gene Name

AIM2

Full Name

Interferon-inducible protein AIM2

Weight

38.954kDa

Superfamily

HIN-200 family

Alternative Names

absent in melanoma 2PYHIN4; AIM2; Gm1313; Ifi210; interferon-inducible protein AIM2; PYHIN4 AIM2 PYHIN4 absent in melanoma 2 interferon-inducible protein AIM2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AIM2, check out the AIM2 Infographic

AIM2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AIM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9683 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury

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5 Customer Q&As for Anti-AIM2 Antibody Picoband™

Question

Can you help my question with product PB9683, anti-AIM2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-15

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9683 anti-AIM2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-15

Question

We are currently using anti-AIM2 antibody PB9683 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

The anti-AIM2 antibody (PB9683) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-24

Question

I was wanting to use your anti-AIM2 antibody for WB for rat spleen on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat spleen identification?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

You can see on the product datasheet, PB9683 anti-AIM2 antibody has been tested for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat spleen in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-08

Question

Will anti-AIM2 antibody PB9683 work for WB with spleen?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

According to the expression profile of spleen, AIM2 is highly expressed in spleen. So, it is likely that anti-AIM2 antibody PB9683 will work for WB with spleen.

Boster Scientific Support

Answered: 2019-07-01

Question

I am interested in to test anti-AIM2 antibody PB9683 on rat spleen for research purposes, then I may be interested in using anti-AIM2 antibody PB9683 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-01-02

Answer

The products we sell, including anti-AIM2 antibody PB9683, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-01-02

Size

Conjugation

Total: $370

SKU:PB9683

In stock, 2 left.

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PB9683
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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