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- Table of Contents
Pycard antibody (A00362-4) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Flow Cytometry analysis of RAW264.7 cells using anti-TMS1/ASC/PYCARD antibody (A00362-4).
Overlay histogram showing RAW264.7 cells stained with A00362-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMS1/ASC/PYCARD Antibody (A00362-4, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing RAW264.7 cells stained with A00362-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMS1/ASC/PYCARD Antibody (A00362-4, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-TMS1/ASC/Pycard Antibody Picoband®
Pycard antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
Pycard antibody was positively detected by WB in:
rat lung tissue, rat spleen tissue, rat thymus tissue, rat heart tissue, mouse lung tissue, mouse spleen tissue, mouse thymus tissue, mouse heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Pycard antibody was positively detected by IHC in:
mouse intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Pycard antibody was positively detected by FC in:
RAW2647 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-TMS1/ASC/Pycard Antibody Picoband®
View all PYCARD Antibodies
View all PYCARD Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse TMS1/ASC/PYCARD recombinant protein (Position: R3-S193).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22-25 kDa
Calculated Molecular Weight
21.5 kDa
Background of Pycard
PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-TMS1/ASC/Pycard Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00362-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
83%
Moderate Homology
Cow
75%
Moderate Homology
Sheep
74%
Moderate Homology
Human
72%
Moderate Homology
Rabbit
71%
Moderate Homology
Monkey
70%
Moderate Homology
Dog
69%
Low homology
Zebrafish
34%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Pycard antibody
Immunohistochemistry (IHC) protocol for Pycard antibody
Flow Cytometry (FC) protocol for Pycard antibody
Publications
Application:
Species:
Protective Effects of Cyclosporin H Against Sepsis-Induced Acute Kidney Injury via Modulation of Fpr1 Signaling and Inhibiting Pyroptosis
Published: 2026-03-06
Journal: Research and Reports in Urology
Application: WB
Impact Factor: 2.70
Species: Mouse
PMID: 41821570
TNIP3 Attenuates Colitis by Inhibiting the TAB1/NF-κB/NLRP3 Signaling Pathway in Macrophages
Published: 2025-12-23
Journal: DIGESTIVE DISEASES AND SCIENCES
Application: WB
Impact Factor: 2.50
Species: Mouse
PMID: 41436895
An Integrated Approach of experiment validation and Single-cell RNA sequencing reveal dual-pathway inhibition of NLRP3/ferroptosis by flavonoids of Sanyeqing fibrous root in acute lung injury
Published: 2025-10-18
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IF,WB
Impact Factor: 5.40
Species: Mouse,Human
PMID: 41115482
Endothelial HMGB1-AIM2 axis worsens myocardial ischemia—reperfusion injury by regulating endothelial pyroptosis
Published: 2025-08-14
Journal: APOPTOSIS
Application: IF,WB
Impact Factor: 8.10
Species: Mouse
PMID: 40813539
Olfactory ensheathing cells transplantation alleviates cancer pain by inhibiting P2X7 receptor expression mediated activation of microglia
Published: 2025-07-09
Journal: International Journal of Surgery
Application: WB
Impact Factor: 10.10
Species: Rat
PMID: 40638362
Pathophysiology-Directed Engineering of a Combination Nanoanalgesic for Neuropathic Pain
Published: 2024-12-24
Journal: Advanced Science
Application: WB
Impact Factor: 14.30
Species: Rat
PMID: 39716944
Vaccarin alleviates septic cardiomyopathy by potentiating NLRP3 palmitoylation and inactivation
Published: 2024-05-31
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 6.70
Species: Rat
PMID: 38851101
Carnosine ameliorates postoperative cognitive dysfunction of aged rats by limiting astrocytes pyroptosis
Published: 2024-04-25
Journal: Neurotherapeutics
Application: WB
Impact Factor: 5.60
Species: Rat
PMID: 38664193
Phoenixin 20 ameliorates pulmonary arterial hypertension via inhibiting inflammation and oxidative stress
Published: 2024-03-19
Journal: Aging-US
Application: WB
Impact Factor: 3.90
Species: Rat
PMID: 38517365
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Product Promise
We promise Anti-TMS1/ASC/Pycard Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00362-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026