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- Table of Contents
APOE antibody (PB9327) performs well in Flow Cytometry, IF, IHC, WB, ELISA (Cap) and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of HepG2 cells using anti-Apolipoprotein E antibody (PB9327).
Overlay histogram showing HepG2 cells stained with PB9327 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Apolipoprotein E Antibody (PB9327,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HepG2 cells stained with PB9327 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Apolipoprotein E Antibody (PB9327,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Apolipoprotein E/APOE Antibody Picoband®
APOE antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IF, IHC, WB, ELISA (Cap) |
Tested Applications
APOE antibody was positively detected by WB in:
human placenta tissue, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
APOE antibody was positively detected by IHC in:
Human Placenta tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
APOE antibody was positively detected by IF in:
human placenta tissue
APOE antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunofluorescence
5 μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
ELISA (Cap)
1-5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Apolipoprotein E/APOE Antibody Picoband®
View all APOE Antibodies
View all APOE Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Apolipoprotein E recombinant protein (Position: K19-H317). Human Apolipoprotein E shares 73% and 72% amino acid (aa) sequence identity with mouse and rat Apolipoprotein E, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated Molecular Weight
36.2 kDa
Background of APOE
APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-Apolipoprotein E/APOE Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9327)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
93%
High Homology
Rabbit
76%
Moderate Homology
Cow
72%
Moderate Homology
Mouse
71%
Moderate Homology
Rat
71%
Moderate Homology
Sheep
70%
Moderate Homology
Dog
70%
Moderate Homology
Zebrafish
26%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for APOE antibody
Immunohistochemistry (IHC) protocol for APOE antibody
Immunofluorescence (IF) protocol for APOE antibody
Flow Cytometry (FC) protocol for APOE antibody
Publications
Application:
Species:
Phaseolus vulgaris Erythroagglutinin (PHA-E)-Positive Ceruloplasmin Acts as a Potential Biomarker in Pancreatic Cancer Diagnosis
Published: 2022-08-08
Journal: Cells
Application: WB
Impact Factor: 7.67
Species: Human
PMID: 35954297
Identification of Apolipoprotein E as a Potential Diagnostic Biomarker of Nasopharyngeal Carcinoma
Published: 2020-09-24
Journal: Cancer Management and Research
Application: WB,IHC
Impact Factor: 2.89
Species: Human
PMID: 33061590
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Product Promise
We promise Anti-Apolipoprotein E/APOE Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, WB, ELISA (Cap). If it doesn't perform as promised, we will refund the product. Learn more
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PB9327
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026