Product Info Summary
SKU: | PB9327 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Apolipoprotein E/APOE Antibody Picoband™
SKU/Catalog Number
PB9327
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Apolipoprotein E/APOE Antibody Picoband™ catalog # PB9327. Tested in ELISA, Flow Cytometry, IHC, IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apolipoprotein E/APOE Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9327)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Apolipoprotein E recombinant protein (Position: K19-H317). Human Apolipoprotein E shares 73% and 72% amino acid (aa) sequence identity with mouse and rat Apolipoprotein E, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9327 is reactive to APOE in Human
Applications
PB9327 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
36 kDa
Calculated molecular weight
36.154kDa
Background of APOE
APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunofluorescence, 5 μg/ml
Flow Cytometry, 1-3μg/1x106 cells
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9327).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein E antigen affinity purified polyclonal antibody (Catalog # PB9327) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein E at approximately 36 kDa. The expected band size for Apolipoprotein E is at 36 kDa.
Click image to see more details
Figure 2. IHC analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9327).
Apolipoprotein E was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Apolipoprotein E Antibody (PB9327) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IF analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9327).
Apolipoprotein E was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Apolipoprotein E Antibody (PB9327) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 4. Flow Cytometry analysis of HepG2 cells using anti-Apolipoprotein E antibody (PB9327).
Overlay histogram showing HepG2 cells stained with PB9327 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Apolipoprotein E Antibody (PB9327,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 5. Sandwich ELISA - Recombinant human Apolipoprotein E/APOE protein standard curve.
Use in combination with reagents from human APOE ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1455).
Protein Target Info & Infographic
Gene/Protein Information For APOE (Source: Uniprot.org, NCBI)
Gene Name
APOE
Full Name
Apolipoprotein E
Weight
36.154kDa
Superfamily
apolipoprotein A1/A4/E family
Alternative Names
APOE; Apo-E; Apolipoprotein E; apolipoprotein E3; late onset); MGC1571 APOE AD2, APO-E, ApoE4, LDLCQ5, LPG apolipoprotein E apolipoprotein E|apolipoprotein E3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APOE, check out the APOE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APOE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Apolipoprotein E/APOE Antibody Picoband™ (PB9327)
Hello CJ!
PB9327 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xue Y,Huang S,Huang J,Li S,Zhang C,Zhou X.Identification of Apolipoprotein E as a Potential Diagnostic Biomarker of Nasopharyngeal Carcinoma.Cancer Manag Res.2020 Sep 24;12:8943-8950.doi:10.2147/CMAR. S239479.PMID:33061590;PMCID:PMC7522425.
Species: Human
PB9327 usage in article: APP:WB, SAMPLE:NPC TISSUES, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-Apolipoprotein E/APOE Antibody Picoband™
Question
The PB9327 product page notes that it has no cross reactivity with other proteins, does that include mouse and rat APOE? Or does this refer to non-APOE proteins?
Verified customer
Asked: 2021-11-12
Answer
Our lab hasn't detected APOE in mouse and rat samples in WB and IHC using the Anti-Apolipoprotein E/APOE Antibody Picoband™ (PB9327).
Boster Scientific Support
Answered: 2021-11-16
Question
Is there a BSA free version of anti-Apolipoprotein E/APOE antibody PB9327 available?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Apolipoprotein E/APOE antibody PB9327 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-20
Question
My question regarding product PB9327, anti-Apolipoprotein E/APOE antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9327 anti-Apolipoprotein E/APOE antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-26
Question
We were well pleased with the WB result of your anti-Apolipoprotein E/APOE antibody. However we have observed positive staining in left adrenal gland secreted. using this antibody. Is that expected? Could you tell me where is APOE supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
From literature, left adrenal gland does express APOE. Generally APOE expresses in secreted. Regarding which tissues have APOE expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 6897404, 7068630, 6860692
Cerebellum, Pubmed ID: 14702039
Cerebrospinal fluid, Pubmed ID: 19838169
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-02-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left adrenal gland using anti-Apolipoprotein E/APOE antibody PB9327. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-07
Question
My question regards to test anti-Apolipoprotein E/APOE antibody PB9327 on human left adrenal gland for research purposes, then I may be interested in using anti-Apolipoprotein E/APOE antibody PB9327 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Krishna
Verified customer
Asked: 2020-01-20
Answer
The products we sell, including anti-Apolipoprotein E/APOE antibody PB9327, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-20
Question
I was wanting to use using your anti-Apolipoprotein E/APOE antibody for negative regulation of triglyceride metabolic process studies. Has this antibody been tested with western blotting on hepg2 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
Thank you for your inquiry. This PB9327 anti-Apolipoprotein E/APOE antibody is tested on human placenta tissue, tissue lysate, hepg2 cells. It is guaranteed to work for ELISA, Flow Cytometry, IHC-P, IHC-F, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-24
Question
I see that the anti-Apolipoprotein E/APOE antibody PB9327 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-29
Question
Is this PB9327 anti-Apolipoprotein E/APOE antibody reactive to the isotypes of APOE?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
The immunogen of PB9327 anti-Apolipoprotein E/APOE antibody is E.coli-derived human Apolipoprotein E recombinant protein (Position: K19-H317). Human Apolipoprotein E shares 73% and 72% amino acid (aa) sequence identity with mouse and rat Apolipoprotein E, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-08
Question
We have seen staining in human cerebellum. Do you have any suggestions? Is anti-Apolipoprotein E/APOE antibody supposed to stain cerebellum positively?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
From literature cerebellum does express APOE. From Uniprot.org, APOE is expressed in left adrenal gland, cerebellum, eye, blood, cerebrospinal fluid, liver, among other tissues. Regarding which tissues have APOE expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 6897404, 7068630, 6860692
Cerebellum, Pubmed ID: 14702039
Cerebrospinal fluid, Pubmed ID: 19838169
Eye, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-10-01
Question
Would PB9327 anti-Apolipoprotein E/APOE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
You can see on the product datasheet, PB9327 anti-Apolipoprotein E/APOE antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-11
Question
I have attached the WB image, lot number and protocol we used for left adrenal gland using anti-Apolipoprotein E/APOE antibody PB9327. Please let me know if you require anything else.
R. Parker
Verified customer
Asked: 2019-01-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-01
Question
Our lab used your anti-Apolipoprotein E/APOE antibody for IHC-F on left adrenal gland in the past. I am using human, and We want to use the antibody for ELISA next. My lab would like examining left adrenal gland as well as cerebrospinal fluid in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
I looked at the website and datasheets of our anti-Apolipoprotein E/APOE antibody and I see that PB9327 has been tested on human in both IHC-F and ELISA. Thus PB9327 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-08-23
Question
We are currently using anti-Apolipoprotein E/APOE antibody PB9327 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-14
Answer
The anti-Apolipoprotein E/APOE antibody (PB9327) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-14
Question
I was wanting to use your anti-Apolipoprotein E/APOE antibody for ELISA for human left adrenal gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human left adrenal gland identification?
C. Johnson
Verified customer
Asked: 2017-04-21
Answer
You can see on the product datasheet, PB9327 anti-Apolipoprotein E/APOE antibody has been validated for ELISA, Flow Cytometry, IHC-P, IHC-F, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human left adrenal gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-21
Question
Would anti-Apolipoprotein E/APOE antibody PB9327 work for ELISA with left adrenal gland?
A. Walker
Verified customer
Asked: 2015-08-28
Answer
According to the expression profile of left adrenal gland, APOE is highly expressed in left adrenal gland. So, it is likely that anti-Apolipoprotein E/APOE antibody PB9327 will work for ELISA with left adrenal gland.
Boster Scientific Support
Answered: 2015-08-28
Question
Is a blocking peptide available for product anti-Apolipoprotein E/APOE antibody (PB9327)?
J. Krishna
Verified customer
Asked: 2015-05-07
Answer
We do provide the blocking peptide for product anti-Apolipoprotein E/APOE antibody (PB9327). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-05-07