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Product Info Summary
| SKU: | A00085-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB, ELISA (Cap) |
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Product info
Product Name
Anti-PCSK9 Antibody Picoband®
SKU/Catalog Number
A00085-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PCSK9 Antibody Picoband® catalog # A00085-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PCSK9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00085-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PCSK9 recombinant protein (Position: S153-Q344).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00085-1 is reactive to PCSK9 in Human
Observed Molecular Weight
66 kDa, 80 kDa (precursor)
Calculated molecular weight
74.3 kDa
Background of PCSK9
Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, PCSK9 was mapped to chromosome 1p32. This gene is a crucial player in the regulation of plasma cholesterol homeostasis. It may prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. PCSK9 can induce ubiquitination of LDLR leading to its subsequent degradation. This gene is involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00085-1 is guaranteed for IHC, WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
ELISA(Cap), 1-5μg/ml, Human
Positive Control
WB: human A431 whole cell, human Hela whole cell, human CACO-2 whole cell, human HepG2 whole cell, human Jurkat whole cell
IHC: human liver cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of PCSK9 using anti-PCSK9 antibody (A00085-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK9 antigen affinity purified polyclonal antibody (Catalog # A00085-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK9 at approximately 66 kDa, 80 kDa (precursor). The expected band size for PCSK9 is at 72 kDa.
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IHC analysis of PCSK9 using anti PCSK9 antibody (A00085-1).
PCSK9 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PCSK9 Antibody (A00085-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Sandwich ELISA - Recombinant human PCSK9 protein standard curve.
Use in combination with reagents from Human PCSK9 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1147).
Specific Publications For Anti-PCSK9 Antibody Picoband® (A00085-1)
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Customer Q&As
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5 Customer Q&As for Anti-PCSK9 Antibody Picoband®
Question
We have observed staining in mouse right lobe of liver. Do you have any suggestions? Is anti-PCSK9 antibody supposed to stain right lobe of liver positively?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
According to literature right lobe of liver does express PCSK9. According to Uniprot.org, PCSK9 is expressed in right lobe of liver, cerebellum teratocarcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have PCSK9 expression, here are a few articles citing expression in various tissues:
Cerebellum, and Teratocarcinoma, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Boster Scientific Support
Answered: 2019-09-16
Question
Our team were happy with the WB result of your anti-PCSK9 antibody. However we have seen positive staining in right lobe of liver cytoplasm. secreted. endosome. lysosome. using this antibody. Is that expected? Could you tell me where is PCSK9 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
According to literature, right lobe of liver does express PCSK9. Generally PCSK9 expresses in cytoplasm. secreted. endosome. lysosome. Regarding which tissues have PCSK9 expression, here are a few articles citing expression in various tissues:
Cerebellum, and Teratocarcinoma, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Boster Scientific Support
Answered: 2019-06-24
Question
We are currently using anti-PCSK9 antibody A00085-1 for human tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
The anti-PCSK9 antibody (A00085-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-30
Question
We have tried in the past anti-PCSK9 antibody for IHC-P on cervix carcinoma in the past. I am using human, and We want to use the antibody for ELISA next. you antibody examining cervix carcinoma as well as cerebellum teratocarcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
I took a look at the website and datasheets of our anti-PCSK9 antibody and I see that A00085-1 has been tested on human in both IHC-P and ELISA. Thus A00085-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-06-21
Question
you antibody using your anti-PCSK9 antibody for negative regulation of low-density lipoprotein particle clearance studies. Has this antibody been tested with western blotting on k562 whole cell lysates? We would like to see some validation images before ordering.
H. Zhao
Verified customer
Asked: 2015-10-30
Answer
Thank you for your inquiry. This A00085-1 anti-PCSK9 antibody is tested on human hela, hela whole cell lysates, hepg2 whole cell lysates, a549 whole cell lysates, k562 whole cell lysates, rat lung tissue, brain tissue, mouse brain. It is guaranteed to work for ELISA, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-10-30
