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Product Info Summary
| SKU: | PA1793 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Apoptosis repressor with CARD/NOL3 Antibody Picoband®
SKU/Catalog Number
PA1793
BA3764-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Apoptosis repressor with CARD/NOL3 Antibody catalog # PA1793. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apoptosis repressor with CARD/NOL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1793)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg Thimerosal, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1793 is reactive to NOL3 in Human, Rat
Observed Molecular Weight
27 kDa
Calculated molecular weight
22.6 kDa
Background of NOL3
NOL3 (Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al. (1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with CASP1, CASP3, or CASP9.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1793 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: human MCF-7 whole cell, human PC-3 whole cel, human Hela whole cell, human SiHa whole cell, rat heart tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of NOL3 using anti-NOL3 antibody (A02831-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: rat heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOL3 antigen affinity purified polyclonal antibody (A02831-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOL3 at approximately 27 kDa. The expected band size for NOL3 is at 23 kDa.
Specific Publications For Anti-Apoptosis repressor with CARD/NOL3 Antibody Picoband® (PA1793)
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4 Customer Q&As for Anti-Apoptosis repressor with CARD/NOL3 Antibody Picoband®
Question
Does anti-Apoptosis repressor with CARD/NOL3 antibody PA1793 work on feline WB with liver?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
Our lab technicians have not validated anti-Apoptosis repressor with CARD/NOL3 antibody PA1793 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Apoptosis repressor with CARD/NOL3 antibody PA1793 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-25
Question
I see that the anti-Apoptosis repressor with CARD/NOL3 antibody PA1793 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-12
Question
We are currently using anti-Apoptosis repressor with CARD/NOL3 antibody PA1793 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
M. Rodriguez
Verified customer
Asked: 2014-02-04
Answer
The anti-Apoptosis repressor with CARD/NOL3 antibody (PA1793) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-02-04
Question
Is a blocking peptide available for product anti-Apoptosis repressor with CARD/NOL3 antibody (PA1793)?
V. Edwards
Verified customer
Asked: 2013-01-15
Answer
We do provide the blocking peptide for product anti-Apoptosis repressor with CARD/NOL3 antibody (PA1793). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-01-15
