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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human Elafin/PI3 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Elafin/PI3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PI3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Elafin/PI3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1117)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A23-Q117
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1117 is reactive to PI3 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK1117 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1117-CAP||Anti-Human PI3 Pre-coated 96-well strip microplate||1|
|EK1117-ST||Human PI3 Standard||2 vials, 10 ng/tube|
|EK1117-DA||Human PI3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Elafin/PI3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Trappin-2/Elafin/Skalp, Dilution ratio of 1:1, concentration in serum is around 800pg/ml.
Some articles we found to cite concentrations of Trappin-2/Elafin/Skalp in samples: 16355004 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Elafin/PI3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Trappin-2/Elafin/Skalp in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For PI3 (Source: Uniprot.Org, NCBI)
cementoin; Elafin; Elastase-specific inhibitor; ESI; ESIWAP four-disulfide core domain protein 14; Peptidase inhibitor 3; peptidase inhibitor 3, skin-derived; PI3; PI-3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); Protease inhibitor WAP3; SKALP; SKALPELAFIN; Skin-derived antileukoproteinase; Trappin2; Trappin-2; WAP four-disulfide core domain 14; WAP3MGC13613; WFDC14elafin PI3 ESI, SKALP, WAP3, WFDC14, cementoin peptidase inhibitor 3 elafin|PI-3|WAP four-disulfide core domain 14|WAP four-disulfide core domain protein 14|elastase-specific inhibitor|peptidase inhibitor 3, skin-derived|pre-elafin|protease inhibitor 3, skin-derived (SKALP)|protease inhibitor WAP3|skin-derived antileukoproteinase|trappin-2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PI3, check out the PI3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PI3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK1117
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