Anti-AQP12 Antibody

Aquaporin-12A antibody

Boster Bio Anti-AQP12 Antibody (Catalog # A19520). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A19520
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-AQP12 Antibody

View all Aquaporin-12A Antibodies

SKU/Catalog Number

A19520

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-AQP12 Antibody (Catalog # A19520). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AQP12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A19520)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human AQP12.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A19520 is reactive to AQP12A in Human

Applications

A19520 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

31.475kDa

Background of Aquaporin-12A

Aquaporins facilitate the transport of water and small neutral solutes across cell membranes By similarity.

Itoh T., Biochem. Biophys. Res. Commun. 330:832-838(2005).
Ishibashi K., Publishers, New York. (2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For AQP12A (Source: Uniprot.org, NCBI)

Gene Name

AQP12A

Full Name

Aquaporin-12A

Weight

31.475kDa

Superfamily

MIP/aquaporin (TC 1.A.8) family

Alternative Names

AQP-12; AQP12AQPX2aquaporin X2; aquaporin 12; aquaporin 12A; aquaporin-12A AQP12A AQP-12, AQP12, AQPX2 aquaporin 12A aquaporin-12A|aquaporin 12|aquaporin X2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AQP12A, check out the AQP12A Infographic

AQP12A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AQP12A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-AQP12 Antibody

Question

I see that the anti-AQP12 antibody A19520 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-05

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-05

Question

My question regarding product A19520, anti-AQP12 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A19520 anti-AQP12 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-01

Question

We are currently using anti-AQP12 antibody A19520 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?

C. Jones

Verified customer

Asked: 2019-07-18

Answer

The anti-AQP12 antibody (A19520) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-18

Question

Does anti-AQP12 antibody A19520 work for WB with body of pancreas?

G. Johnson

Verified customer

Asked: 2018-07-19

Answer

According to the expression profile of body of pancreas, AQP12A is highly expressed in body of pancreas. So, it is likely that anti-AQP12 antibody A19520 will work for WB with body of pancreas.

Boster Scientific Support

Answered: 2018-07-19

Question

Does anti-AQP12 antibody A19520 work on goat WB with body of pancreas?

Verified Customer

Verified customer

Asked: 2018-02-14

Answer

Our lab technicians have not validated anti-AQP12 antibody A19520 on goat. You can run a BLAST between goat and the immunogen sequence of anti-AQP12 antibody A19520 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat body of pancreas in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-02-14

Question

I was wanting to use your anti-AQP12 antibody for WB for human body of pancreas on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human body of pancreas identification?

J. Taylor

Verified customer

Asked: 2017-11-13

Answer

You can see on the product datasheet, A19520 anti-AQP12 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human body of pancreas in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-11-13

Question

Here is the WB image, lot number and protocol we used for body of pancreas using anti-AQP12 antibody A19520. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-10-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-11

Order DetailsPrice
A19520-100uL

100uL

$360

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