Product Info Summary
SKU: | A19520 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-AQP12 Antibody
View all Aquaporin-12A Antibodies
SKU/Catalog Number
A19520
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-AQP12 Antibody (Catalog # A19520). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AQP12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A19520)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human AQP12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A19520 is reactive to AQP12A in Human
Applications
A19520 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
31.475kDa
Background of Aquaporin-12A
Aquaporins facilitate the transport of water and small neutral solutes across cell membranes By similarity.
Itoh T., Biochem. Biophys. Res. Commun. 330:832-838(2005).
Ishibashi K., Publishers, New York. (2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat cells, using AQP12 antibody A19520.
Protein Target Info & Infographic
Gene/Protein Information For AQP12A (Source: Uniprot.org, NCBI)
Gene Name
AQP12A
Full Name
Aquaporin-12A
Weight
31.475kDa
Superfamily
MIP/aquaporin (TC 1.A.8) family
Alternative Names
AQP-12; AQP12AQPX2aquaporin X2; aquaporin 12; aquaporin 12A; aquaporin-12A AQP12A AQP-12, AQP12, AQPX2 aquaporin 12A aquaporin-12A|aquaporin 12|aquaporin X2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AQP12A, check out the AQP12A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AQP12A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AQP12 Antibody (A19520)
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No publications found for A19520
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-AQP12 Antibody
Question
I see that the anti-AQP12 antibody A19520 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-05
Question
My question regarding product A19520, anti-AQP12 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A19520 anti-AQP12 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-01
Question
We are currently using anti-AQP12 antibody A19520 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
C. Jones
Verified customer
Asked: 2019-07-18
Answer
The anti-AQP12 antibody (A19520) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-18
Question
Does anti-AQP12 antibody A19520 work for WB with body of pancreas?
G. Johnson
Verified customer
Asked: 2018-07-19
Answer
According to the expression profile of body of pancreas, AQP12A is highly expressed in body of pancreas. So, it is likely that anti-AQP12 antibody A19520 will work for WB with body of pancreas.
Boster Scientific Support
Answered: 2018-07-19
Question
Does anti-AQP12 antibody A19520 work on goat WB with body of pancreas?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
Our lab technicians have not validated anti-AQP12 antibody A19520 on goat. You can run a BLAST between goat and the immunogen sequence of anti-AQP12 antibody A19520 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat body of pancreas in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-14
Question
I was wanting to use your anti-AQP12 antibody for WB for human body of pancreas on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human body of pancreas identification?
J. Taylor
Verified customer
Asked: 2017-11-13
Answer
You can see on the product datasheet, A19520 anti-AQP12 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human body of pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-13
Question
Here is the WB image, lot number and protocol we used for body of pancreas using anti-AQP12 antibody A19520. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-11