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Product Info Summary
| SKU: | M06167 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IP, ICC, WB |
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Product info
Product Name
Anti-ARAP1 Purified Monoclonal Antibody
SKU/Catalog Number
M06167
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ARAP1 Purified Monoclonal Antibody (Catalog# M06167). Tested in IP, WB, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-ARAP1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06167)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
ARAP1-2
Isotype
Mouse IgG1
Immunogen
His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1. The antibody ARAP1-2 reacts with C-terminal part of ARAP1 (intracellular epitope), a 160 kDa adaptor protein.
Cross-reactivity
Cross-reactivity with CLIC5b was not determined.
Reactive Species
M06167 is reactive to ARAP1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
162.2 kDa
Background of ARAP1
ARAP1 (angiotensin II type 1 receptor-associated protein) is an adaptor protein with ArfGAP and RhoGAP activities, containing five PH domains and ankyrin repeate. This adaptor seems to serve as a link between phosphoinositide- Arf-, and Rho-mediated cell signaling. ARAP1 supports the plasma membrane recycling of angiotensin II type 1 receptor (AT1) and is important also for cell-specific trafficking of pro-death receptor TRAIL-R1 (DR4) to the plasma membrane, thus promoting the TRAIL-induced apoptosis in certain cell types. ARAP1 also affects cell spreading.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06167 is guaranteed for IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 2 μg/ml
Validation Images & Assay Conditions
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Immunocytochemistry detection of colocalization of ARAP1 with DR4 at the plasma membrane and in early endosomes. ARAP1-transfected NCTC cells were treated with TRAIL for 0´, 30´ or 60´ and fixed with methanol. DR4 was detected by rabbit polyclonal (red) and ARAP1 with ARAP1-2 mouse monoclonal antibody (green). Arrowheads show ARAP1-DR4 colocalization at the plasma membrane and intracellular membranes, whereas arrows show their colocalization in early endosomes.
Specific Publications For Anti-ARAP1 Purified Monoclonal Antibody (M06167)
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