Product Info Summary
SKU: | M00189 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Argonaute 2 AGO2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00189
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Argonaute 2 AGO2 Rabbit Monoclonal Antibody catalog # M00189. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Argonaute 2 AGO2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00189)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AODE-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Argonaute 2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00189 is reactive to AGO2 in Human, Mouse, Rat
Applications
M00189 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
97.208kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Argonaute 2 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For AGO2 (Source: Uniprot.org, NCBI)
Gene Name
AGO2
Full Name
Protein argonaute-2
Weight
97.208kDa
Superfamily
argonaute family
Alternative Names
Protein argonaute-2 AGO2 CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10 argonaute RISC catalytic component 2 protein argonaute-2|PAZ Piwi domain protein|argonaute 2, RISC catalytic component|cancer susceptibility candidate 7 (non-protein coding)|eukaryotic translation initiation factor 2C, 2|long intergenic non-protein coding RNA 980|protein slicer
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AGO2, check out the AGO2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AGO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Argonaute 2 AGO2 Rabbit Monoclonal Antibody (M00189)
Hello CJ!
M00189 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Therapeutic Potential of OMe-PS-miR-29b1 for Treating Liver Fibrosis
Xin X,Kumar V,Lin F,Kumar V,Bhattarai R,Bhatt VR,Tan C,Mahato RI. Redox-responsive nanoplatform for codelivery of miR-519c and gemcitabine for pancreatic cancer therapy. Sci Adv.2020 Nov 11;6(46):eabd6764.doi:10.1126/sciadv.abd6764.PMID:33177098.
Species: Human
M00189 usage in article: APP:WB, SAMPLE:PACA-2 CELLS, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Argonaute 2 AGO2 Rabbit Monoclonal Antibody
Question
We are currently using anti-Argonaute 2 Rabbit Monoclonal antibody M00189 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The anti-Argonaute 2 Rabbit Monoclonal antibody (M00189) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-08
Question
We have been able to see staining in human brain eye. Are there any suggestions? Is anti-Argonaute 2 Rabbit Monoclonal antibody supposed to stain brain eye positively?
E. Anderson
Verified customer
Asked: 2020-03-18
Answer
According to literature brain eye does express AGO2. According to Uniprot.org, AGO2 is expressed in forebrain, brain eye, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have AGO2 expression, here are a few articles citing expression in various tissues:
Brain, and Eye, Pubmed ID: 15489334
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Boster Scientific Support
Answered: 2020-03-18
Question
I was wanting to use your anti-Argonaute 2 Rabbit Monoclonal antibody for WB for human brain eye on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain eye identification?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
It shows on the product datasheet, M00189 anti-Argonaute 2 Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain eye in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-04
Question
I was wanting to use using your anti-Argonaute 2 Rabbit Monoclonal antibody for small interfering rna (sirna) biogenesis studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
Thanks for your inquiry. This M00189 anti-Argonaute 2 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-30
Question
We ordered your anti-Argonaute 2 Rabbit Monoclonal antibody for ICC on brain eye in the past. I am using human, and We want to use the antibody for IF next. My question regards examining brain eye as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
I have checked the website and datasheets of our anti-Argonaute 2 Rabbit Monoclonal antibody and I see that M00189 has been validated on human in both ICC and IF. Thus M00189 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-17
Question
I see that the anti-Argonaute 2 Rabbit Monoclonal antibody M00189 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-31
Question
Will M00189 anti-Argonaute 2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
It shows on the product datasheet, M00189 anti-Argonaute 2 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-28
Question
Does anti-Argonaute 2 Rabbit Monoclonal antibody M00189 work for WB with brain eye?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
According to the expression profile of brain eye, AGO2 is highly expressed in brain eye. So, it is likely that anti-Argonaute 2 Rabbit Monoclonal antibody M00189 will work for WB with brain eye.
Boster Scientific Support
Answered: 2019-09-24
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain eye using anti-Argonaute 2 Rabbit Monoclonal antibody M00189. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-07
Question
My question regards to test anti-Argonaute 2 Rabbit Monoclonal antibody M00189 on human brain eye for research purposes, then I may be interested in using anti-Argonaute 2 Rabbit Monoclonal antibody M00189 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
The products we sell, including anti-Argonaute 2 Rabbit Monoclonal antibody M00189, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-19
Question
Our lab were happy with the WB result of your anti-Argonaute 2 Rabbit Monoclonal antibody. However we have observed positive staining in brain eye cytoplasm using this antibody. Is that expected? Could you tell me where is AGO2 supposed to be expressed?
S. Gonzalez
Verified customer
Asked: 2019-03-20
Answer
From what I have seen in literature, brain eye does express AGO2. Generally AGO2 expresses in cytoplasm, p-body. Regarding which tissues have AGO2 expression, here are a few articles citing expression in various tissues:
Brain, and Eye, Pubmed ID: 15489334
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Boster Scientific Support
Answered: 2019-03-20
Question
I have a question about product M00189, anti-Argonaute 2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00189 anti-Argonaute 2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-21
Question
See attached the WB image, lot number and protocol we used for brain eye using anti-Argonaute 2 Rabbit Monoclonal antibody M00189. Please let me know if you require anything else.
R. Patel
Verified customer
Asked: 2018-10-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-08
Question
Is this M00189 anti-Argonaute 2 Rabbit Monoclonal antibody reactive to the isotypes of AGO2?
Verified Customer
Verified customer
Asked: 2017-12-27
Answer
The immunogen of M00189 anti-Argonaute 2 Rabbit Monoclonal antibody is A synthesized peptide derived from human Argonaute 2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-27
Question
Is a blocking peptide available for product anti-Argonaute 2 Rabbit Monoclonal antibody (M00189)?
S. Thomas
Verified customer
Asked: 2016-09-16
Answer
We do provide the blocking peptide for product anti-Argonaute 2 Rabbit Monoclonal antibody (M00189). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-09-16
Question
Is there a BSA free version of anti-Argonaute 2 Rabbit Monoclonal antibody M00189 available?
J. Krishna
Verified customer
Asked: 2016-08-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Argonaute 2 Rabbit Monoclonal antibody M00189 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-08-11