radio
SKU MA1039
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number GP1.4
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC

Overview

Product Name Anti-Episialin, EMA Antibody (Monoclonal, GP1.4)
SKU/Catalog Number MA1039
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Episialin, EMA, mucin 1, cell surface associated (MUC1) detection. Tested with IHC-P, IHC-F in Human. No cross reactivity with other proteins.
Cite This Product Anti-Episialin, EMA Antibody (Monoclonal, GP1.4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1039)
Host Mouse
Immunogen Human milk fat globule membranes.
Reactivity Human

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, By Heat
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/ma1039 1 IHC anti episialin ema antibody monoclonal gp1 4.jpg

Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

/antibody/ma1039 2 IHC anti episialin ema antibody monoclonal gp1 4.jpg

Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Lung Cancer Tissue

/antibody/ma1039 3 IHC anti episialin ema antibody monoclonal gp1 4.jpg

Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P15941
Gene Name MUC1
Protein Name Mucin-1
Tissue Specificity Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only. .
Alternative Names Mucin-1;MUC-1;Breast carcinoma-associated antigen DF3;Cancer antigen 15-3;CA 15-3;Carcinoma-associated mucin;Episialin;H23AG;Krebs von den Lungen-6;KL-6;PEMT;Peanut-reactive urinary mucin;PUM;Polymorphic epithelial mucin;PEM;Tumor-associated epithelial membrane antigen;EMA;Tumor-associated mucin;CD227;Mucin-1 subunit alpha;MUC1-NT;MUC1-alpha;Mucin-1 subunit beta;MUC1-beta;MUC1-CT;MUC1;PUM;
Subcellular Localization Apical cell membrane ; Single- pass type I membrane protein . Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
Molecular Weight 122102 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
Background MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1(EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type I transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.

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Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
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Troubleshooting

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Publications

Globose, cystic olfactory ensheathing cell tumor: A case report and literature review
Establishment of a new representative model of human ovarian cancer in mice

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to muc1 antibody, ema antibody, kl-6 antibody, muc-1 antibody, mucin 1 antibody, epithelial membrane antigen antibody