Data & Images
|Product Name||Anti-Episialin, EMA Antibody (Monoclonal, GP1.4)|
|Description||Mouse IgG monoclonal antibody for Episialin, EMA, mucin 1, cell surface associated (MUC1) detection. Tested with IHC-P, IHC-F in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-Episialin, EMA Antibody (Monoclonal, GP1.4) GP1.4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1039)|
|Replacement Item||This antibody may replace the following items: sc-10899|sc-10897|sc-20796|sc-55531|sc-48362|sc-1066|sc-271566|sc-1665|sc-271176|sc-166774|sc-65594|sc-1063|sc-56334|sc-71810|sc-71808|sc-71809|sc-71807|sc-136010|sc-130333|sc-1063-G|sc-1066-G|sc-1076-G|sc-101809|sc-101810|sc-10897|sc-10899|sc-12948-R|sc-12949|sc-12949-R|sc-130333|sc-136010|sc-20796|sc-48362|sc-55531 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Human milk fat globule membranes.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||122102 MW|
|Protein Function||The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.|
|Tissue Specificity||Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only. .|
|Sequence Similarities||Contains 1 SEA domain.|
|Subcellular Localization||Apical cell membrane ; Single- pass type I membrane protein . Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.|
|Alternative Names||Mucin-1;MUC-1;Breast carcinoma-associated antigen DF3;Cancer antigen 15-3;CA 15-3;Carcinoma-associated mucin;Episialin;H23AG;Krebs von den Lungen-6;KL-6;PEMT;Peanut-reactive urinary mucin;PUM;Polymorphic epithelial mucin;PEM;Tumor-associated epithelial membrane antigen;EMA;Tumor-associated mucin;CD227;Mucin-1 subunit alpha;MUC1-NT;MUC1-alpha;Mucin-1 subunit beta;MUC1-beta;MUC1-CT;MUC1;PUM;|
|Research Areas||tags & cell markers|cell type markers|tumor associated|signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|muc|cancer|invasion/microenvironment|tumor immunology|tumor-associated antigens||
Background for Mucin-1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Episialin, EMA Antibody (Monoclonal, GP1.4) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, By Heat
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Episialin, EMA Antibody (Monoclonal, GP1.4) Images
Click the images to enlarge.
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Mammary Cancer Tissue