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Overview
| Product Name | Anti-Episialin, EMA Antibody (Monoclonal, GP1.4) |
|---|---|
| SKU/Catalog Number | MA1039 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for Episialin, EMA, mucin 1, cell surface associated (MUC1) detection. Tested with IHC-P, IHC-F in Human. No cross reactivity with other proteins. |
| Cite This Product | Anti-Episialin, EMA Antibody (Monoclonal, GP1.4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1039) |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Immunogen | Human milk fat globule membranes. |
| Reactivity | Human |
Assay Details
Assay Dilutions Overview
Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, By Heat
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, By Heat
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).Images And Assay Conditions
Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P15941 |
|---|---|
| Gene Name | MUC1, MUC1 |
| Protein Name | Mucin-1 |
| Tissue Specificity | Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only. . |
| Alternative Names | Mucin-1;MUC-1;Breast carcinoma-associated antigen DF3;Cancer antigen 15-3;CA 15-3;Carcinoma-associated mucin;Episialin;H23AG;Krebs von den Lungen-6;KL-6;PEMT;Peanut-reactive urinary mucin;PUM;Polymorphic epithelial mucin;PEM;Tumor-associated epithelial membrane antigen;EMA;Tumor-associated mucin;CD227;Mucin-1 subunit alpha;MUC1-NT;MUC1-alpha;Mucin-1 subunit beta;MUC1-beta;MUC1-CT;MUC1;PUM; |
| Subcellular Localization | Apical cell membrane ; Single- pass type I membrane protein . Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions. |
| Molecular Weight | 122102 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. |
|---|---|
| Research Areas | Cancer, Cell Type Markers, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Tags & Cell Markers, Tumor Associated, Tumor Immunology, Tumor-Associated Antigens *You can search these to find other products in these research areas. |
| Background | MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1(EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type I transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. |
Other Recommended Resources
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Publications
Globose, cystic olfactory ensheathing cell tumor: A case report and literature review
Establishment of a new representative model of human ovarian cancer in mice
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to muc1 antibody, ema antibody, kl-6 antibody, muc-1 antibody, mucin 1 antibody, epithelial membrane antigen antibody