Anti-Episialin, EMA Antibody (Monoclonal, GP1.4)

SKU MA1039
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number GP1.4
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC

Overview

Product Name Anti-Episialin, EMA Antibody (Monoclonal, GP1.4)
SKU/Catalog Number MA1039
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Episialin, EMA, mucin 1, cell surface associated (MUC1) detection. Tested with IHC-P, IHC-F in Human. No cross reactivity with other proteins.
Cite This Product Anti-Episialin, EMA Antibody (Monoclonal, GP1.4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1039)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Human milk fat globule membranes.
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, By Heat
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Lung Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P15941
Gene Name MUC1
Protein Name Mucin-1
Tissue Specificity Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only. .
Alternative Names Mucin-1;MUC-1;Breast carcinoma-associated antigen DF3;Cancer antigen 15-3;CA 15-3;Carcinoma-associated mucin;Episialin;H23AG;Krebs von den Lungen-6;KL-6;PEMT;Peanut-reactive urinary mucin;PUM;Polymorphic epithelial mucin;PEM;Tumor-associated epithelial membrane antigen;EMA;Tumor-associated mucin;CD227;Mucin-1 subunit alpha;MUC1-NT;MUC1-alpha;Mucin-1 subunit beta;MUC1-beta;MUC1-CT;MUC1;PUM;
Subcellular Localization Apical cell membrane ; Single- pass type I membrane protein . Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
Molecular Weight 122102 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
Research Areas Human

*You can search these to find other products in these research areas.
Background MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1(EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type I transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Monoclonal clone# G2 antibody for MUC1, MUC1 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MUC1, MUC1 information: Molecular Weight: 122102 MW; Subcellular Localization: Apical cell membrane ; Single- pass type I membrane protein . Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions; Tissue Specificity: Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only.
Write Your Own Review
Only registered users can write reviews. Please Sign in or create an account
In stock
Order Product
MA1039
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

troubleshooting_box_image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Publications

Globose, cystic olfactory ensheathing cell tumor: A case report and literature review
Zhang J, Chen X, Shi G, Xie X, Liu H, Zhang X, Lai Y, Zuo Y, Chen Z, Liu S, Wang H. J Ovarian Res. 2013 Feb 6;6(1):9. Doi: 10.1186/1757-2215-6-9. Establishment Of A New Representative Model Of Human Ovarian Cancer In Mice.

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to muc1 antibody, ema antibody, kl-6 antibody, muc-1 antibody, mucin 1 antibody, epithelial membrane antigen antibody
Write a review for MA1039
Copyright © 2019 Boster Biological Technology Inc. All rights reserved.