Overview
| Product Name |
Anti-Episialin, EMA MUC1 Antibody (Monoclonal, GP1.4)
See all MUC1 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
MA1039 |
| Pack Size |
100μg/vial |
| Storage & Handling |
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Description |
Monoclonal clone# G2 antibody for MUC1, MUC1 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MUC1, MUC1 information: Molecular Weight: 122102 MW; Subcellular Localization: Apical cell membrane ; Single- pass type I membrane protein . Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions; Tissue Specificity: Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only. |
| Cite This Product |
Anti-Episialin, EMA MUC1 Antibody (Monoclonal, GP1.4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1039)
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Anti-Episialin, EMA MUC1 Antibody (Monoclonal, GP1.4) may replace the following items: sc 10899|sc 10897|sc 20796|sc 55531|sc 48362|sc 1066|sc 271566|sc 1665|sc 271176|sc 166774|sc 65594|sc 1063|sc 56334|sc 71810|sc 71808|sc 71809|sc 71807|sc 136010|sc 130333|sc 1063 G|sc 1066 G|sc 1076 G|sc 101809|sc 101810|sc 10897|sc 10899|sc 12948 R|sc 12949|sc 12949 R|sc 130333|sc 136010|sc 20796|sc 48362|sc 55531. |
Product Specs
| Host |
Mouse |
| Reactivity/Species |
Human |
| Applications |
IHC
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
Human milk fat globule membranes. |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For MUC1 (Source: Uniprot.org, NCBI)
| Uniprot Id | P15941 |
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| NCBI Gene Id | 4582 |
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| Species Of This Entry | Human |
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| Gene Name | MUC1 |
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| Protein Name | Mucin-1 |
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| Alternative Names | MUC1|Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227 antigen; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; MAM6; MUC-1; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMMUC-1/SEC; PEMT; Polymorphic epithelial mucin; PUMMUC-1/X; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin |
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| Molecular Weight | 122102 |
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| Post Tranlational Modifications | Glycosylation; Phosphorylation; Cleavage; Deglycosylation; Methylation; Biotinylation; Oxidation; Acetylation; Sulphation; Hydroxylation; Dephosphorylation; Ubiquitination; Deacetylation; Palmitoylation; Sumoylation; Acylation; Reduction |
|---|
| Subcellular Localization | Apical cell membrane; Single-pass type I membrane protein. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.; [Isoform 5]: Secreted.; [Isoform Y]: Secreted.; [Isoform 9]: Secreted.; [Mucin-1 subunit beta]: Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalize |
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| Gene Location | 1q22, on Chromasome 1, gene sequence: NC_000001.11 (155185824..155192915, complement) |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MUC1 (Source: Uniprot.org, NCBI)
| Protein Function | The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. |
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| Tissue Specificity | Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only. |
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| Interacting Proteins | ABL1; CEBPB; EGFR; MET; U2AF2 |
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| Research Areas | Cancer; Extracellular Matrix |
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| Related Diseases | Malignant Neoplasms; Neoplasms; Carcinoma; Malignant Neoplasm Of Breast; Adenocarcinoma; Mammary Neoplasms; Neoplasm Metastasis; Malignant Paraganglionic Neoplasm; Pancreatic Neoplasm; Tissue Adhesions; Cell Invasion; Pneumonia; Malignant Neoplasm Of Pancreas; Mucinous Adenocarcinoma; Lung Neoplasms; Lung Diseases, Interstitial; Malignant Neoplasm Of Lung; Fibrosis; Ovarian Neoplasm; Lung Diseases |
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| Related Pathways | Glycosylation; Immune Response; Localization; Secretion; Pathogenesis; Cell Adhesion; Invasive Growth; Cell Proliferation; Cell Growth; Cell-cell Adhesion; Hypersensitivity; Cell Cycle; Cell Differentiation; Transport; Angiogenesis; Conjugation; Filamentous Growth; Embryo Implantation; Humoral Immune Response; Cell Death |
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| Background | MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1(EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type I transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. |
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| Scientific References | PMID: 2394722 by Lan M.S., et al. Cloning and sequencing of a human pancreatic tumor mucin cDNA.
PMID: 2318825 by Ligtenberg M.J.L., et al. Episialin, a carcinoma-associated mucin, is generated by a polymorphic gene encoding splice variants with alternative amino termini. |
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Our Boster Quality Guarantee for Anti-Episialin, EMA MUC1 Antibody (Monoclonal, GP1.4) covers its use in the following applications.
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, By Heat
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Lung Cancer Tissue
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all MUC1 primary antibodies,
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Total number of citations: 2
| Zhang J, Chen X, Shi G, Xie X, Liu H, Zhang X, Lai Y, Zuo Y, Chen Z, Liu S, Wang H. J Ovarian Res. 2013 Feb 6;6(1):9. Doi: 10.1186/1757-2215-6-9. Establishment Of A New Representative Model Of Human Ovarian Cancer In Mice. |
PubMed ID 23384043 |
| Globose, cystic olfactory ensheathing cell tumor: A case report and literature review |
PubMed ID 27895759 |
