Anti-MUC1 Antibody Picoband™

Boster Bio Anti-MUC1 Antibody Picoband™ catalog # A00187. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00187
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-MUC1 Antibody Picoband™

See all MUC1 products

SKU/Catalog Number

A00187

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MUC1 Antibody Picoband™ catalog # A00187. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MUC1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00187)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human MUC1 recombinant protein (Position: S1062-V1139).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00187 is reactive to MUC1 in Human, Mouse, Rat

Applications

A00187 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For MUC1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P15941

Gene ID

4582

Gene Name

MUC1

Full Name

Mucin-1

Weight

122.102kDa

Alternative Names

Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227 antigen; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; MAM6; MUC-1; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMMUC-1/SEC; PEMT; Polymorphic epithelial mucin; PUMMUC-1/X; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin MUC1 ADMCKD, ADMCKD1, ADTKD2, CA 15-3, CD227, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X/ZD, PEM, PEMT, PUM, MUC1 mucin 1, cell surface associated mucin-1|H23 antigen|breast carcinoma-associated antigen DF3|cancer antigen 15-3|carcinoma-associated mucin|episialin|krebs von den Lungen-6|mucin 1, transmembrane|peanut-reactive urinary mucin|polymorphic epithelial mucin|tumor associated epithelial mucin|tumor-associated epithelial membrane antigen

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MUC1, check out the MUC1 Infographic

MUC1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MUC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00187 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Globose, cystic olfactory ensheathing cell tumor: A case report and literature review

Zhang J, Chen X, Shi G, Xie X, Liu H, Zhang X, Lai Y, Zuo Y, Chen Z, Liu S, Wang H. J Ovarian Res. 2013 Feb 6;6(1):9. Doi: 10.1186/1757-2215-6-9. Establishment Of A New Representative Model Of Human Ovarian Cancer In Mice.

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16 Customer Q&As for Anti-MUC1 Antibody Picoband™

Question

I was wanting to use your anti-MUC1 antibody for IHC for mouse body of stomach on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse body of stomach identification?

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

You can see on the product datasheet, A00187 anti-MUC1 antibody has been tested for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse body of stomach in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-10

Question

Does anti-MUC1 antibody A00187 work for IHC with body of stomach?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

According to the expression profile of body of stomach, MUC1 is highly expressed in body of stomach. So, it is likely that anti-MUC1 antibody A00187 will work for IHC with body of stomach.

Boster Scientific Support

Answered: 2020-02-26

Question

you antibody to test anti-MUC1 antibody A00187 on mouse body of stomach for research purposes, then I may be interested in using anti-MUC1 antibody A00187 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

The products we sell, including anti-MUC1 antibody A00187, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-10

Question

My boss were well pleased with the WB result of your anti-MUC1 antibody. However we have been able to see positive staining in lung isoform 5: secreted. using this antibody. Is that expected? Could you tell me where is MUC1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-07

Answer

From what I have seen in literature, lung does express MUC1. Generally MUC1 expresses in apical cell membrane, isoform 5: secreted., isoform y: secreted., isoform 9: secreted., mucin-1 subunit beta: cell membrane. Regarding which tissues have MUC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 15969018
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lung, Pubmed ID: 8604237
Mammary carcinoma, Pubmed ID: 1697589, 11341784
Pancreatic carcinoma, Pubmed ID: 2394722

Boster Scientific Support

Answered: 2019-11-07

Question

Please see the WB image, lot number and protocol we used for body of stomach using anti-MUC1 antibody A00187. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-18

Question

We purchased anti-MUC1 antibody for ELISA on thyroid in a previous experiment. I am using human, and We intend to use the antibody for IHC next. you antibody examining thyroid as well as body of stomach in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

I looked at the website and datasheets of our anti-MUC1 antibody and I see that A00187 has been tested on human in both ELISA and IHC. Thus A00187 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-13

Question

Do you have a BSA free version of anti-MUC1 antibody A00187 available?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MUC1 antibody A00187 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-05

Question

Is a blocking peptide available for product anti-MUC1 antibody (A00187)?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

We do provide the blocking peptide for product anti-MUC1 antibody (A00187). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-17

Question

We have seen staining in mouse thyroid. Are there any suggestions? Is anti-MUC1 antibody supposed to stain thyroid positively?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

Based on literature thyroid does express MUC1. Based on Uniprot.org, MUC1 is expressed in body of stomach, pancreatic carcinoma, mammary carcinoma, carcinoma, cervix carcinoma, thyroid, lung, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MUC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 15969018
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lung, Pubmed ID: 8604237
Mammary carcinoma, Pubmed ID: 1697589, 11341784
Pancreatic carcinoma, Pubmed ID: 2394722

Boster Scientific Support

Answered: 2019-06-13

Question

I have a question about product A00187, anti-MUC1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Jones

Verified customer

Asked: 2019-01-22

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00187 anti-MUC1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-01-22

Question

I see that the anti-MUC1 antibody A00187 works with IHC, what is the protocol used to produce the result images on the product page?

S. Krishna

Verified customer

Asked: 2018-03-08

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-03-08

Question

We are currently using anti-MUC1 antibody A00187 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2017-05-24

Answer

The anti-MUC1 antibody (A00187) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-05-24

Question

Does A00187 anti-MUC1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Kulkarni

Verified customer

Asked: 2016-09-06

Answer

As indicated on the product datasheet, A00187 anti-MUC1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-09-06

Question

you antibody using your anti-MUC1 antibody for negative regulation of cell adhesion mediated by integrin studies. Has this antibody been tested with western blotting on mammary cancer tissue? We would like to see some validation images before ordering.

B. Edwards

Verified customer

Asked: 2016-06-03

Answer

We appreciate your inquiry. This A00187 anti-MUC1 antibody is validated on rat pancreas tissue, lung tissue, mouse pancreas tissue, human placenta tissue, colon cancer tissue, small intestine tissue, mammary cancer tissue, hela whole cell lysates. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-06-03

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for body of stomach using anti-MUC1 antibody A00187. Let me know if you need anything else.

K. Wu

Verified customer

Asked: 2015-12-15

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-12-15

Question

Is this A00187 anti-MUC1 antibody reactive to the isotypes of MUC1?

E. Lewis

Verified customer

Asked: 2013-01-24

Answer

The immunogen of A00187 anti-MUC1 antibody is E. coli-derived human MUC1 recombinant protein (Position: S1062-V1139). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-01-24

Conjugation

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A00187
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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