Anti-Argonaute 4/AGO4 Antibody Picoband™

Ago4 antibody

Boster Bio Anti-Argonaute 4/AGO4 Antibody Picoband™ catalog # PB9979. Tested in WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: PB9979
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Argonaute 4/AGO4 Antibody Picoband™

View all Ago4 Antibodies

SKU/Catalog Number

PB9979

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Argonaute 4/AGO4 Antibody Picoband™ catalog # PB9979. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Argonaute 4/AGO4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9979)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Argonaute 4, identical to the related mouse sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9979 is reactive to Ago4 in Human, Rat

Applications

PB9979 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

115 kDa

Calculated molecular weight

97.037kDa

Background of Ago4

AGO4 (Argonaute 4) is also known as EIF2C4. This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic containing PAZ and PIWI domains, and it may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and eukaryotic translation initiation factor 2C, 1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Ago4 (Source: Uniprot.org, NCBI)

Gene Name

Ago4

Full Name

Protein argonaute-4

Weight

97.037kDa

Superfamily

argonaute family

Alternative Names

Protein argonaute-4 Ago4|5730550L01Rik, AI481660, Eif2c, Eif2c4|argonaute RISC catalytic subunit 4|protein argonaute-4|Piwi/Argonaute family protein meIF2C4|argonaute 4|argonaute RISC catalytic component 4|eukaryotic translation initiation factor 2C, 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Ago4, check out the Ago4 Infographic

Ago4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ago4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Argonaute 4/AGO4 Antibody Picoband™

Question

Is this PB9979 anti-Argonaute 4/AGO4 antibody reactive to the isotypes of AGO4?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

The immunogen of PB9979 anti-Argonaute 4/AGO4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Argonaute 4 (114-153aa KDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALD), identical to the related mouse sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-10

Question

We are currently using anti-Argonaute 4/AGO4 antibody PB9979 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

The anti-Argonaute 4/AGO4 antibody (PB9979) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-20

Question

Would anti-Argonaute 4/AGO4 antibody PB9979 work for WB with frontal cortex?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

According to the expression profile of frontal cortex, AGO4 is highly expressed in frontal cortex. So, it is likely that anti-Argonaute 4/AGO4 antibody PB9979 will work for WB with frontal cortex.

Boster Scientific Support

Answered: 2019-06-10

Question

Would anti-Argonaute 4/AGO4 antibody PB9979 work on feline WB with brain?

Verified Customer

Verified customer

Asked: 2019-04-30

Answer

Our lab technicians have not tested anti-Argonaute 4/AGO4 antibody PB9979 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Argonaute 4/AGO4 antibody PB9979 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-04-30

Question

I see that the anti-Argonaute 4/AGO4 antibody PB9979 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-10-18

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-10-18

Size

Conjugation

Total: $370

SKU:PB9979

In stock, 1 left.

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PB9979
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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