Human IL-1 RL1 / ST2 PicoKine™ Quick ELISA Kit

Human IL-1 RL1 / ST2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human IL1RL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK1116
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 90 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human IL-1 RL1 / ST2 PicoKine™ Quick ELISA Kit

See all ST2/IL-33R products

SKU/Catalog Number

FEK1116

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-1 RL1 / ST2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human IL1RL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-1 RL1 / ST2 PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1116)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: K19-S328

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1116 is reactive to IL1RL1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK1116 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity
FEK1116-CAP 96-well plate precoated with anti-Human ST2/IL-33R antibody 1
FEK1116-ST lyophilized recombinant Human ST2/IL-33R standard 10 ng/tube
FEK1116-DA Biotinylated anti-Human ST2/IL-33R detection antibody (50x) 130 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0140.0810.1350.2230.4110.8341.4251.979

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2421795548624717785061
Standard deviation9.6880.77257.8413.58106.68237.86
CV (%)4%4.5%4.7%5.5%6%4.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ST2/IL-33R in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12422532622472517.442.9%
Sample 21795167915611563164996.635.8%
Sample 354865339521154065360100.781.8%
*number of samples for each test n=16.

Gene/Protein Information For IL1RL1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q01638

Gene ID

9173

Gene Name

IL1RL1

Full Name

Interleukin-1 receptor-like 1

Weight

63.358kDa

Superfamily

interleukin-1 receptor family

Alternative Names

DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed IL1RL1 DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1 interleukin 1 receptor like 1 interleukin-1 receptor-like 1|growth stimulation-expressed|homolog of mouse growth stimulation-expressed|interleukin 1 receptor-related protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL1RL1, check out the IL1RL1 Infographic

IL1RL1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL1RL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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