Human IL-1 RL1 / ST2 ELISA Kit PicoKine™

Human IL-1 RL1 / ST2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL1RL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1116
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human IL-1 RL1 / ST2 ELISA Kit PicoKine™

See all ST2/IL-33R products

SKU/Catalog Number

EK1116

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-1 RL1 / ST2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL1RL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-1 RL1 / ST2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1116)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: K19-S328

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1116 is reactive to IL1RL1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1116 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1116-CAP Anti-Human IL1RL1 Pre-coated 96-well strip microplate 1
EK1116-ST Human IL1RL1 Standard 2 vials, 10 ng/tube
EK1116-DA Human IL1RL1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0020.1140.1760.3330.6670.9981.5432.059

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-1 RL1 / ST2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2421795548624717785061
Standard deviation9.6880.77257.8413.58106.68237.86
CV (%)4%4.5%4.7%5.5%6%4.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ST2/IL-33R in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12422532622472517.442.9%
Sample 21795167915611563164996.635.8%
Sample 354865339521154065360100.781.8%
*number of samples for each test n=16.

Gene/Protein Information For IL1RL1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q01638

Gene ID

9173

Gene Name

IL1RL1

Full Name

Interleukin-1 receptor-like 1

Weight

63.358kDa

Superfamily

interleukin-1 receptor family

Alternative Names

DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed IL1RL1 DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1 interleukin 1 receptor like 1 interleukin-1 receptor-like 1|growth stimulation-expressed|homolog of mouse growth stimulation-expressed|interleukin 1 receptor-related protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL1RL1, check out the IL1RL1 Infographic

IL1RL1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL1RL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1116 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Gungor O, Unal HU, Guclu A, Gezer M, Eyileten T, Guzel FB, Altunoren O, Erken E, Oguz Y, Kocyigit I, Yilmaz MI. PLoS One. 2017 Jun 14;12(6):e0178939. doi: 10.1371/journal.pone.0178939. eCollection 2017. IL-33 and ST2 levels in chronic kidney disea...

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9 Customer Q&As for Human IL-1 RL1 / ST2 ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-07-05

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-07-05

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-03-30

Question

Q: What is the optimal O.D. value for ST2 ELISA kit? I performed your ST2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain ST2 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ST2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of ST2 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-12-30

Question

Q. Is the product for the soluble form of human ST-2?

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

A. Our EK1116 is designed to test human soluble ST-2.

Boster Scientific Support

Answered: 2019-10-22

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

B. Carter

Verified customer

Asked: 2019-09-08

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-09-08

Question

What is the immogen sequence of the the antibody pair used for EK1116?

Verified customer

Asked: 2019-07-01

Answer

For the Human IL-1 RL1 / ST2 ELISA Kit PicoKine™ (EK1116), the sequence of the standard for both the capture and detection antibody is K19-S328.

Boster Scientific Support

Answered: 2019-07-01

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement ST2 using the EK1116 Human ST2 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-01-22

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2018-01-22

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2016-03-13

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-03-13

Question

Q: how to analyze ELISA data? I measured ST2 level in cell lysates.

Verified Customer

Verified customer

Asked: 2014-05-19

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2014-05-19

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