Anti-Aromatase/CYP19A1 Antibody

SKU PA1489
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-Aromatase/CYP19A1 Antibody
SKU/Catalog Number PA1489
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Aromatase(CYP19A1) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-Aromatase/CYP19A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1489)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Aromatase(485-503aa KNMLEMIFTPRNSDRCLEH).
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

Anti-Aromatase antibody, PA1489, Western blotting
Lane 1: Human Placenta Tissue Lysate
Lane 2: Human Placenta Tissue Lysate

Anti-Aromatase antibody, PA1489, IHC(P)
IHC(P): Human Placenta Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P11511
Gene Name CYP19A1
Protein Name Aromatase
Tissue Specificity Brain, placenta and gonads. .
Alternative Names Aromatase;1.14.14.14;CYPXIX;Cytochrome P-450AROM;Cytochrome P450 19A1;Estrogen synthase;CYP19A1;ARO1, CYAR, CYP19;
Subcellular Localization Membrane; Peripheral membrane protein.
Molecular Weight 57883 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
Research Areas Human

*You can search these to find other products in these research areas.
Background Aromatase is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia.

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Polyclonal antibody for AROMATASE/CYP19A1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. AROMATASE/CYP19A1 information: Molecular Weight: 57883 MW; Subcellular Localization: Membrane; Peripheral membrane protein; Tissue Specificity: Brain, placenta and gonads.
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In stock
Order Product
PA1489
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

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Publications

Electroacupuncture enhances extragonadal aromatization in ovariectomized rats
Extragonadal aromatization increases with time after ovariectomy in rats
Zhang, L., Zhang, X., Zhang, X., Lu, Y., Li, L., & Cui, S. (2017). MiRNA-143 mediates the proliferative signaling pathway of FSH and regulates estradiol production. Journal of Endocrinology, 234(1),1-14. doi: 10.1530/JOE-16-0488
Zhang D, Yang B, Zou W, Lu X, Xiong M, Wu L, Wang J, Gao J, Xu S, Zou T. Biomed Res Int. 2013;2013:301540. Doi: 10.1155/2013/301540. Epub 2012 Dec 26. Estradiol Synthesis And Release In Cultured Female Rat Bone Marrow Stem Cells.

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
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