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Product Info Summary
| SKU: | P00791-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00791-1
BM4837 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody catalog # P00791-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00791-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IFC-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-GSK3 beta (Ser9)
Reactive Species
P00791-1 is reactive to GSK3B in Human
Observed Molecular Weight
46 kDa
Calculated molecular weight
46.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00791-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
IHC: human kidney tissue
ICC/IF: HeLa cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of HeLa cells treated with Calyculin A, using Phospho-GSK3 beta (Ser9) Antibody.
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WECP activated Akt/GSK3β/β-Catenin signaling pathway in DPCs. DPCs were incubated with WECP (40, 80 and 160 μg/mL) in culture medium for 24 h. (A) Akt and GSK3β phosphorylation levels were detected in DPCs after WECP treatment. (B) Effects of WECP treatment on β-Catenin and Wnt10b protein expression levels in DPCs. (C) Transcriptional expression of LEF1, IGF1, and VEGF in DPCs detected using RT-PCR. Data are presented as means ± SD of three independent replicates. * p < 0.05, ** p < 0.01 vs. control group.
Index in PubMed under a CC BY license. PMID: 36891275
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WECP activated Akt/GSK3β/β-Catenin signaling pathway in denuded mouse skin. (A) Effects of WECP and finasteride treatments on Akt and GSK3β protein phosphorylation in mouse skin. (B) Effects of WECP and finasteride treatments on β-Catenin and Wnt10 translational expression in mouse skin. (C) Effects of WECP and finasteride treatments on transcriptional expression of β-Catenin, Wnt10b, Wnt5a, LEF1, VEGF, and IGF1 in mouse skin. Data are means ± SD of three independent replicates. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group. WECPL, WECPH, and Fin represent low-dose WECP, high-dose WECP, and finasteride treatment, respectively.
Index in PubMed under a CC BY license. PMID: 36891275
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Western blot analysis of GSK3 beta (phospho S9) expression in 293T cell lysates, treated with Calyculin A.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-GSK3 beta (Ser9) Antibody.
Specific Publications For Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody (P00791-1)
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1 Customer Q&As for Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal antibody P00791-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal antibody (P00791-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
