Anti-Phospho-ASAP1 Y782 Antibody

Product Name

Anti-Phospho-ASAP1 Y782 Antibody

View all Asap1 Antibodies

SKU/Catalog Number

P03848

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-ASAP1 Y782 Antibody (Catalog # P03848). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Phospho-ASAP1 Y782 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03848)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of mouse ASAP1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P03848 is reactive to Asap1 in Human, Mouse

Applications

P03848 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

127.421kDa

Background of Asap1

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. ASAP1 (also known as AMAP1, 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP,ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, Differentiation-enhancing factor 1, DEF-1) is an Arf-directed GTPase activating protein that is a substrate for the kinases Src and FAK and has been implicated in the regulation of membrane traffic, focal adhesions and invadopodia/podosomes. Phosphorylation of ASAP1 at tyrosine 782 has been found to affect enzymatic and some biological activities, including the function of invadopodia. ASAP1 is expressed in many tissues but is most abundant in the testis, brain, lung and spleen. A heightened expression was seen in the adipose tissue from obese (ob) and diabetic (db) animals. Multiple transcript variants have been reported for this protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:4,000 - 1:16,000
IHC: 20-40 µg/ml
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Phosphorylated ASAP1 was detected in immunocytochemical sections of mouse NIH3T3 cells transfected with activated Src using rabbit anti-ASAP1 pY782 Antigen Affinity purified polyclonal antibody (Catalog # P03848). Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.

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ASAP1 was detected in paraffin-embedded sections of epidermal keratinocytes in human skin using rabbit anti-ASAP1 pY782 Antigen Affinity purified polyclonal antibody (Catalog # P03848) at 20 μg/mL. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.

Gene/Protein Information For Asap1 (Source: Uniprot.org, NCBI)

Alternative Names

130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activatingprotein; AMAP1; ARF GTPase-activating protein 1; arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; ArfGAP with SH3 domain, ankyrin repeat and PH domain 1; centaurin, beta 4; CENTB4; DDEF1ADP-ribosylation factor-directed GTPase-activating protein 1; development and differentiation enhancing factor 1; Development and differentiation-enhancing factor 1; Differentiation-enhancing factor 1; KIAA1249DEF-1; PAG2; PAP; PIP2-dependent ARF1 GAP; ZG14P Asap1|AV239055, DEF-1, Ddef1, PAP, mKIAA1249, s19|ArfGAP with SH3 domain, ankyrin repeat and PH domain1|arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1|130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein|130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein|ADP-ribosylation factor-directed GTPase-activating protein 1|ARF GTPase-activating protein 1|PIP2-dependent ARF1 GAP|development and differentiation-enhancing factor 1

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