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- Table of Contents
NLRC4 antibody (PB9673) performs well in IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Western blot analysis of CARD12 using anti-CARD12 antibody (PB9673).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: 22RV1 Whole Cell Lysate,
Lane 2: RH35 Whole Cell Lysate,
Lane 3: NIH3T3 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD12 antigen affinity purified polyclonal antibody (Catalog # PB9673) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD12 at approximately 116 kDa. The expected band size for CARD12 is at 116 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: 22RV1 Whole Cell Lysate,
Lane 2: RH35 Whole Cell Lysate,
Lane 3: NIH3T3 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD12 antigen affinity purified polyclonal antibody (Catalog # PB9673) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD12 at approximately 116 kDa. The expected band size for CARD12 is at 116 kDa.
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Anti-CARD12/NLRC4 Antibody Picoband®
NLRC4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, ICC, WB |
Tested Applications
NLRC4 antibody was positively detected by WB in:
22RV1 Whole Cell, RH35 Whole Cell, NIH3T3 Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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ICC
IF
Human
Recommend
Recommend
Mouse
Recommend
Recommend
Rat
Recommend
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-CARD12/NLRC4 Antibody Picoband®
View all NLRC4 Antibodies
View all NLRC4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CARD12, different from the related mouse sequence by five amino acids, and from the related rat sequence by seven amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
116 kDa
Calculated Molecular Weight
116.2 kDa
Background of NLRC4
NLR family CARD domain-containing protein 4, also known as CARD12, is a protein that in humans is encoded by the NLRC4 gene. It is mapped to 2p22.3. This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-CARD12/NLRC4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9673)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
86%
Moderate Homology
Sheep
84%
Moderate Homology
Rat
81%
Moderate Homology
Cow
81%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NLRC4 antibody
Publications
Application:
Species:
NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology
Published: 2019-07-18
Journal: Cellular & Molecular Immunology
Application: WB
Impact Factor: 8.21
Species: Mouse
PMID: 31320730
Tim-3 exacerbates kidney ischaemia/reperfusion injury through the TLR-4/NF-κB signalling pathway and an NLR-C4 inflammasome activation
Published: 2018-03-23
Journal: CLINICAL AND EXPERIMENTAL IMMUNOLOGY
Application: WB
Impact Factor: 3.54
Species: Mouse
PMID: 29570768
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Product Promise
We promise Anti-CARD12/NLRC4 Antibody Picoband® performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9673
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026