Rabbit IgG polyclonal antibody for Myeloid differentiation primary response protein MyD88(MYD88) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 5 publication(s).
Anti-MyD88 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-MyD88 Antibody
See all MYD88 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for MYD88 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MYD88 information: Molecular Weight: 33233 MW; Subcellular Localization: Cytoplasm ; Tissue Specificity: Ubiquitous.|
|Cite This Product||Anti-MyD88 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1660)|
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|Anti-MyD88 Antibody may replace the following items: sc 8197|sc 8196|sc 11356|sc 74532|sc 136970|sc 31306.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human MyD88(174-188aa FVQEMIRQLEQTNYR), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For MYD88 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||4615|
|Species Of This Entry||Human|
|Protein Name||Myeloid differentiation primary response protein MyD88|
|Alternative Names||MYD88|MyD88; MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Methylation; Ubiquitination; Glycosylation; Phosphorothioation; Acylation; Acetylation; Oxidation; Dephosphorylation; Ribosylation; Biotinylation; Nitration; Sulphation; Nitrosylation; Citrullination; Gpi Anchoring; Farnesylation; Deglycosylation; Myristoylation|
|Subcellular Localization||Cytoplasm. Nucleus.|
|Gene Location||3p22.2, on Chromasome 3, gene sequence: NC_000003.12 (38138661..38143022)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MYD88 (Source: Uniprot.org, NCBI)
|Protein Function||Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:15361868, PubMed:18292575). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:15361868, PubMed:24316379, PubMed:19506249). Increases IL-8 transcription (PubMed:9013863). Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).|
|Interacting Proteins||CYTH2; DOCK8; FADD; HDAC6; IRAK1; IRAK4; SARM1; SIAH1; SMAD3; SPOP; STAP2; TIRAP; TLR2; TLR4; TNFRSF13B; TXN; USP7|
|Related Diseases||Inflammation; Innate Immune Response; Infective Disorder; Inflammatory Response; Bacterial Infections; Pneumonia; Neoplasms; Systemic Infection; Virus Diseases; Malignant Neoplasms; Autoimmune Reaction; Tuberculosis; Colitis; Autoimmune Diseases; Salmonella Infections; Cell Invasion; Autoimmunity; Fibrosis; Tissue Adhesions; Influenza|
|Related Pathways||Immune Response; Secretion; Cytokine Production; Innate Immune Response; Pathogenesis; Inflammatory Response; Cell Activation; Cell Death; Cell Proliferation; Virulence; Phagocytosis; Adaptive Immune Response; Macrophage Activation; Localization; Cytokine Secretion; Rna Interference; Response To Lipopolysaccharide; Chemokine Production; Cell Differentiation; T Cell Proliferation|
|Background||MYD88(MYELOID DIFFERENTIATION PRIMARY RESPONSE GENE 88), is a protein that, in humans, is encoded by the MYD88 gene. MyD88 is a key downstream adapter for most Toll-like receptors(TLRs) and interleukin-1 receptors(IL1Rs). And it is mapped on 3p22.2. MYD88 encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. Qverexpression of MYD88 caused an increase in the level of transcription from the interleukin-8 promoter. The C-terminal domain of MYD88 has significant sequence similarity to the cytoplasmic domain of IL1RAP. Inhibiting the IL1R-MYD88 pathway in vivo could block the damage from acute inflammation that occurs in response to sterile cell death, and do so in a way that might not compromise tissue repair or host defense against pathogens.|
|Scientific References||PMID: 8957090 by Hardiman G., et al. Molecular characterization and modular analysis of human MyD88.|
PMID: 9013863 by Bonnert T.P., et al. The cloning and characterization of human MyD88: a member of an IL-1 receptor related family.
Our Boster Quality Guarantee for Anti-MyD88 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: RAJI Cell Lysate
IHC(P): Human Tonsil Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 5
|Toll-Like Receptor 4 Prompts Human Breast Cancer Cells Invasiveness via Lipopolysaccharide Stimulation and Is Overexpressed in Patients with Lymph Node Metastasis||PubMed ID 25299052|
|High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy||PubMed ID 26468333|
|Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway||PubMed ID 27164829|
|Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response||PubMed ID 29442011|
|Immunoregulatory role of MicroRNA-21 in macrophages in response to bacillus calmette-guerin infection involves modulation of the TLR4/MyD88 signaling pathway||PubMed ID 28494464|
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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