SKU MA1114
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number GTU-88
Host Mouse
Ig Isotype Mouse IgG1
Applications ICC, WB

Overview

Product Name Anti-Gamma-Tubulin Antibody (Monoclonal, GTU-88)
SKU/Catalog Number MA1114
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for gamma-Tubulin, golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA1; GGA2; GGA3 ) detection. Tested with WB, ICC in Human,mouse,rat. No cross reactivity with other proteins.
Cite This Product Anti-Gamma-Tubulin Antibody (Monoclonal, GTU-88) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1114)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Form Lyophilized
Immunogen Synthetic gamma-tubulin peptide(amino acids 38-53), conjugated to KLH.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunocytochemistry , 1μg/mlμg/ml, Human, mouse, rat, -
Western blot, 0.25-0.5μg/mlμg/ml, Human, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Images And Assay Conditions

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Boster Kit Box

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id D3ZHG8
Gene Name GGA1; GGA2; GGA3
Protein Name golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3
Tissue Specificity GGA1: Ubiquitously expressed.
Alternative Names Golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Predicted) ;Protein Gga3 ;Gga3 ;Gga3_predicted ;rCG_32821 ;
Subcellular Localization GGA1: Golgi apparatus, trans-Golgi networkmembrane; Peripheral membrane protein. Endosome membrane;Peripheral membrane protein.|Gga1: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane |GGA2: Golgi apparatus, trans-Golgi networkmembrane |Gga2: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane |GGA3: Golgi apparatus, trans-Golgi networkmembrane |Gga3: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane
Molecular Weight 71715 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function GGA1: Plays a role in protein sorting and trafficking betweenthe trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and bindsubiquitinated proteins and membrane cargo molecules with acytosolic acidic cluster-dileucine (AC-LL) motif.|GGA2: Plays a role in protein sorting and trafficking betweenthe trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and bindsubiquitinated proteins and membrane cargo molecules with acytosolic acidic cluster-dileucine (AC-LL) motif.|GGA3: Plays a role in protein sorting and trafficking betweenthe trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and bindsubiquitinated proteins and membrane cargo molecules with acytosolic acidic cluster-dileucine (AC-LL) motif.
Research Areas Golgi Proteins, Protein Trafficking, Signal Transduction

*You can search these to find other products in these research areas.
Background Gamma-tubulin is a universal component of microtubule organizing centers, which is essential for nuclear division and microtubule assembly in Aspergillus nidulans. TUBG1 mapped to within 20 kb of TUBG2 at 17q21. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens.

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Order Product (MA1114)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240

USD $240

Ships next business day.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is tubg1 antibody