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Product Info Summary
| SKU: | MA1114 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ICC, WB |
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Product info
Product Name
Anti-Gamma-Tubulin Antibody (Monoclonal, GTU-88)
SKU/Catalog Number
MA1114
BM1606 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Gamma-Tubulin Antibody (Monoclonal, GTU-88) catalog # MA1114. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Gamma-Tubulin Antibody (Monoclonal, GTU-88) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1114)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
GTU-88
Isotype
Mouse IgG1
Immunogen
Synthetic gamma-tubulin peptide (amino acids 38-53), conjugated to KLH.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1114 is reactive to TUBG1 in Human, Mouse, Rat
Observed Molecular Weight
55 kDa
Calculated molecular weight
51.2 kDa
Background of TUBG1
Gamma-tubulin is a universal component of microtubule organizing centers, which is essential for nuclear division and microtubule assembly in Aspergillus nidulans. TUBG1 mapped to within 20 kb of TUBG2 at 17q21. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1114 is guaranteed for ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 1μg/mlμg/ml, Human, mouse, rat, -
Western blot, 0.25-0.5μg/mlμg/ml, Human, mouse, rat
Positive Control
WB: human Hela whole cell, human COLO-320 whole cell, human HepG2 whole cell, human U-87MG whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Gamma-Tubulin using anti-Gamma-Tubulin antibody (MA1114).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Gamma-Tubulin antigen affinity purified monoclonal antibody (Catalog # MA1114) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Gamma-Tubulin at approximately 55 kDa. The expected band size for Gamma-Tubulin is at 55 kDa.
Specific Publications For Anti-Gamma-Tubulin Antibody (Monoclonal, GTU-88) (MA1114)
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