Product Info Summary
SKU: | A00916T71 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IHC, WB |
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Product info
Product Name
Anti-ATF2 (phospho-T71) Antibody
SKU/Catalog Number
A00916T71
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ATF2 (phospho-T71) Antibody catalog # A00916T71. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATF2 (phospho-T71) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00916T71)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human ATF-2 around the phosphorylation site of T69.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00916T71 is reactive to ATF2 in Human, Mouse, Rat
Applications
A00916T71 is guaranteed for IP, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54.537kDa
Background of ATF2
Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.
Gotoh I., J. Biol. Chem. 276:4276-4286(2001).
Bloem L.J., J. Mol. Cell. Cardiol. 33:1739-1750(2001).
Gross E.A., J. Biol. Chem. 277:13873-13882(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IP: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunohistochemistry validation of ATF2 using Anti-ATF2 (phospho-T71) Antibody (A00916T71).
Immunohistochemistry (IHC) analyzes of p-ATF2 (T71) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
Protein Target Info & Infographic
Gene/Protein Information For ATF2 (Source: Uniprot.org, NCBI)
Gene Name
ATF2
Full Name
Cyclic AMP-dependent transcription factor ATF-2
Weight
54.537kDa
Superfamily
bZIP family
Alternative Names
activating transcription factor 2formerly; ATF2; cAMP responsive element binding protein 2; cAMP-responsive element-binding protein 2; CREB2; CREBP1; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; HB16; MGC111558; mXBP; TREB7 ATF2 CRE-BP1, CREB-2, CREB2, HB16, TREB7 activating transcription factor 2 cyclic AMP-dependent transcription factor ATF-2|activating transcription factor 2 splice variant ATF2-var2|cAMP response element-binding protein CRE-BP1|cAMP responsive element binding protein 2, formerly|cAMP-dependent transcription factor ATF-2|cyclic AMP-responsive element-binding protein 2|histone acetyltransferase ATF2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATF2, check out the ATF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATF2 (phospho-T71) Antibody (A00916T71)
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No publications found for A00916T71
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