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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (citrate).|
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Mouse P-Selectin / CD62P ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse P-Selectin / CD62P ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Selp in cell culture supernatants, serum and plasma (citrate). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse P-Selectin / CD62P ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0506)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: W42-A709
312 pg/ml - 20,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0506 is reactive to Selp in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (citrate).
EK0506 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of P-Selectin/CD62P
P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1). The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.
|EK0506-CAP||Anti-Mouse Selp Pre-coated 96-well strip microplate||1|
|EK0506-ST||Mouse Selp Standard||2 vials, 20 ng/tube|
|EK0506-DA||Mouse Selp Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse P-Selectin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse P-Selectin / CD62P ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of P-Selectin/CD62P in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Selp (Source: Uniprot.Org, NCBI)
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP Selp|CD62, CD62P, GMP-140, Gr, Grmp, LECAM3, P-sel, PADGEM|selectin, platelet|P-selectin|CD62 antigen-like family member P|granule membrane protein 140|leukocyte-endothelial cell adhesion molecule 3|platelet activation dependent granule-external membrane protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Selp, check out the Selp Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Selp: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0506 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of the eukaryotic initiation factor 6 gene on expression levels of inflammatory mediators in M2 macrophages during scar repair Retraction in /10.3892/mmr.2017.7190
Simvastatin Inhibits Inflammation in Ischemia-Reperfusion Injury
Guo J,Li M,Yang Y,Zhang L,Zhang LW,Sun QY.Pretreatment with atorvastatin ameliorates cobra venom factor-induced acute lung inflammation in mice.BMC Pulm Med.2020 Oct 12;20(1):263.doi:10.1186/s12890-020-01307-3.PMID:33046059;PMCID:PMC7552367.
EK0506 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA
Tun X, Yasukawa K, Yamada K. Free Radic Biol Med. 2014 Sep;74:108-17. Doi: 10.1016/J.Freeradbiomed.2014.06.020. Epub 2014 Jun 30. Involvement Of Nitric Oxide With Activation Of Toll-Like Receptor 4 Signaling In Mice With Dextran Sodium Sulfate-Ind...
Yasukawa K, Tokuda H, Tun X, Utsumi H, Yamada K. Free Radic Res. 2012 Dec;46(12):1427-36. Doi: 10.3109/10715762.2012.732698. Epub 2012 Oct 9. The Detrimental Effect Of Nitric Oxide On Tissue Is Associated With Inflammatory Events In The Vascular E...
Li L, Ben Y, Yuan S, Liu A, Wu H, Xu J, Zhang X. Emerg Microbes Infect. 2013 Jul;2(7):E42. Doi: 10.1038/Emi.2013.42. Epub 2013 Jul 10. Recruitment Of Hiv-1 Target Cells At Topical Mucosal Sites: A Sensitive And Early Marker For Determining The Saf...
Zhong M, He B, Yang J, Bao R, Zhang Y, Zhou D, Chen Y, Li L, Han C, Yang Y, Sun Y, Cao Y, Li Y, Shi W, Jiang S, Zhang X, Yan H. Antimicrob Agents Chemother. 2012 Jun;56(6):3121-32. Doi: 10.1128/Aac.05950-11. Epub 2012 Mar 5. L-Selectin And P-Selec...
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