Mouse P-Selectin / CD62P ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse P-Selectin. 96wells/kit, with removable strips. Cited in 4 publication(s).
Mouse P-Selectin / CD62P ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum and plasma (citrate).|
|Sample Volume:||100μl per well|
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|Product Name||Mouse P-Selectin / CD62P ELISA Kit PicoKine™
See all Selp primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse P Selectin/SELP with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma (citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. P Selectin/SELP tissue specificity: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.|
|Cite This Product||Mouse P-Selectin / CD62P ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0506)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum and plasma (citrate).|
|Immunogen||Expression system for standard: NSO; Immunogen sequence: W42-A709|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Protein Target Info (Source: Uniprot.org)
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. .|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Kit Components And Assay QC Data Details Of Mouse P-Selectin / CD62P ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0506-CAP||96-well plate precoated with anti-Mouse Selp antibody||1|
|EK0506-ST||lyophilized recombinant Mouse Selp standard||20ng/tube|
|EK0506-DA||biotinylated anti-Mouse Selp antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0506 Mouse P-Selectin / CD62P ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse P-Selectin / CD62P ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse P-Selectin / CD62P ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 4
|Zhong M, He B, Yang J, Bao R, Zhang Y, Zhou D, Chen Y, Li L, Han C, Yang Y, Sun Y, Cao Y, Li Y, Shi W, Jiang S, Zhang X, Yan H. Antimicrob Agents Chemother. 2012 Jun;56(6):3121-32. Doi: 10.1128/Aac.05950-11. Epub 2012 Mar 5. L-Selectin And P-Selec...||PubMed ID 22391529|
|Li L, Ben Y, Yuan S, Liu A, Wu H, Xu J, Zhang X. Emerg Microbes Infect. 2013 Jul;2(7):E42. Doi: 10.1038/Emi.2013.42. Epub 2013 Jul 10. Recruitment Of Hiv-1 Target Cells At Topical Mucosal Sites: A Sensitive And Early Marker For Determining The Saf...||PubMed ID 26038476|
|Yasukawa K, Tokuda H, Tun X, Utsumi H, Yamada K. Free Radic Res. 2012 Dec;46(12):1427-36. Doi: 10.3109/10715762.2012.732698. Epub 2012 Oct 9. The Detrimental Effect Of Nitric Oxide On Tissue Is Associated With Inflammatory Events In The Vascular E...||PubMed ID 22998024|
|Tun X, Yasukawa K, Yamada K. Free Radic Biol Med. 2014 Sep;74:108-17. Doi: 10.1016/J.Freeradbiomed.2014.06.020. Epub 2014 Jun 30. Involvement Of Nitric Oxide With Activation Of Toll-Like Receptor 4 Signaling In Mice With Dextran Sodium Sulfate-Ind...||PubMed ID 24992835|
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Note: sample size takes longer to process, average 5-7 days.
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