Anti-CD62P/Selp Antibody Picoband™

P-Selectin/CD62P antibody

Boster Bio Anti-CD62P/Selp Antibody Picoband™ catalog # A01241-1. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A01241-1
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC, ICC, WB

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Product Name

Anti-CD62P/Selp Antibody Picoband™

View all P-Selectin/CD62P Antibodies

SKU/Catalog Number

A01241-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD62P/Selp Antibody Picoband™ catalog # A01241-1. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD62P/Selp Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01241-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse CD62P recombinant protein (Position: W42-A267).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01241-1 is reactive to SELP in Mouse, Rat

Applications

A01241-1 is guaranteed for ELISA, Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

110 kDa

Calculated molecular weight

90.834kDa

Background of P-Selectin/CD62P

CD62P is also known as SELP or P-selectin. This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For SELP (Source: Uniprot.org, NCBI)

Gene Name

SELP

Full Name

P-selectin

Weight

90.834kDa

Superfamily

selectin/LECAM family

Alternative Names

CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP SELP CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL selectin P P-selectin|CD62 antigen-like family member P|GMP-140|granule membrane protein 140|granule membrane protein 140kDa|granulocyte membrane protein|leukocyte-endothelial cell adhesion molecule 3|platelet activation dependent granule-external membrane protein|platelet alpha-granule membrane protein|selectin P (granule membrane protein 140kDa, antigen CD62)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SELP, check out the SELP Infographic

SELP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SELP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01241-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Fabrication of biomolecule-PEG micropattern on titanium surface and its effects on platelet adhesion

Zhang R,Ji J,Zhou X,Li R.Irisin Pretreatment Protects Kidneys against Acute Kidney Injury Induced by Ischemia/Reperfusion via Upregulating the Expression of Uncoupling Protein 2. Biomed Res Int.2020 Aug 31;2020:6537371.doi:10.1155/2020/6537371.PMID:329349
Species: Human,Mouse
A01241-1 usage in article: APP:IHC, SAMPLE:KIDNEY TISSUE, DILUTION:1:100

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1 Customer Q&As for Anti-CD62P/Selp Antibody Picoband™

Question

Is the protocol for A01241-1 done with live cells or fixed cells? In addition, this antibody is unconjugated and a labeled 2nd antibody is required for detection. However, Our sample are platelets. Is it okay for wash after incubation with primary antibody, followed by 2nd antibody incubation and wash-steps with such kind of sample? Have you tested in FC with platelets using this anti-CD62P antibody?

Verified customer

Asked: 2020-06-24

Answer

The Anti-CD62P/Selp Antibody Picoband™ (A01241-1) protocol is done with fixed cells. We haven't tested in FC with platelets using this anti-CD62P antibody. We recommend to work on PBMC. If you want to work on platelets, please follow the provided protocol for the product

Boster Scientific Support

Answered: 2020-06-29

Order DetailsPrice
A01241-1

100μg

$370
A01241-1-10ug

10μg sample (liquid)

$99
A01241-1-Biotin

100 μg Biotin conjugated

$570
A01241-1-Cy3

100 μg Cy3 conjugated

$570
A01241-1-Dylight488

100 μg Dylight488 conjugated

$570
A01241-1-Dylight550

100 μg Dylight550 conjugated

$570
A01241-1-Dylight594

100 μg Dylight594 conjugated

$570
A01241-1-FITC

100 μg FITC conjugated

$570
A01241-1-HRP

100 μg HRP conjugated

$570
A01241-1-APC

100 μg APC conjugated

$670
A01241-1-PE

100 μg PE conjugated

$670
A01241-1-carrier-free

Carrier Free

$370

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A01241-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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