Click image to see more details
Product Info Summary
| SKU: | A01241-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD62P/Selp Antibody Picoband®
SKU/Catalog Number
A01241-1
Size
100 μg/vial
Form
Lyophilized
Description
SELP (P-selectin/CD62P) is an adhesion molecule stored in platelets/endothelial cells that binds PSGL-1 and mediates neutrophil adhesion and leukocyte rolling—key early steps in inflammation and thrombosis. Assay context: ELISA/flow cytometry/WB enable complementary readouts (soluble/total vs. surface expression), with tissue/vascular correlation often supported by IHC in vascular beds and confirmatory Western blotting. Frequently interpreted with other vascular inflammation/adhesion markers such as ICAM1 and acute inflammatory mediators (e.g., Human IL-12 p70 ELISA) depending on study design.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD62P/Selp Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01241-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CD62P recombinant protein (Position: W42-A267).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01241-1 is reactive to Selp in Mouse, Rat
Observed Molecular Weight
110 kDa
Calculated molecular weight
83.1 kDa
Background of Selp
CD62P is also known as SELP or P-selectin. This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01241-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse heart tissue, rat heart tissue
FCM: RAW2647 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CD62P using anti-CD62P antibody (A01241-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates,
Lane 2: rat heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD62P antigen affinity purified polyclonal antibody (Catalog # A01241-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD62P at approximately 110KD. The expected band size for CD62P is at 91KD.
Click image to see more details
Flow Cytometry analysis of RAW264.7 cells using anti-CD62P antibody (A01241-1).
Overlay histogram showing RAW264.7 cells stained with A01241-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD62P Antibody (A01241-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-CD62P/Selp Antibody Picoband® (A01241-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD62P/Selp Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CD62P/Selp Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-CD62P/Selp Antibody Picoband®
Question
Is the protocol for A01241-1 done with live cells or fixed cells? In addition, this antibody is unconjugated and a labeled 2nd antibody is required for detection. However, Our sample are platelets. Is it okay for wash after incubation with primary antibody, followed by 2nd antibody incubation and wash-steps with such kind of sample? Have you tested in FC with platelets using this anti-CD62P antibody?
Verified customer
Asked: 2020-06-24
Answer
The Anti-CD62P/Selp Antibody Picoband™ (A01241-1) protocol is done with fixed cells. We haven't tested in FC with platelets using this anti-CD62P antibody. We recommend to work on PBMC. If you want to work on platelets, please follow the provided protocol for the product
Boster Scientific Support
Answered: 2020-06-29
