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Product Info Summary
| SKU: | A00371 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ATF4 Antibody Picoband®
SKU/Catalog Number
A00371
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ATF4 Antibody Picoband® catalog # A00371. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00371)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ATF4, different from the related mouse and rat sequences by five amino acids.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00371 is reactive to ATF4 in Human
Observed Molecular Weight
50 kDa
Calculated molecular weight
38.6 kDa
Background of ATF4
ATF4, Activating Transcription Factor 4, is also known as CREB2. ATF4 belongs to the large ATF/CREB family of transcription factors which bind DNA via their basic region and dimerize via their leucine zipper domain to form a variety of homo- and heterodimers to regulate gene transcription. It is identified that members of this family share significant sequence similarity within a leucine zipper DNA-binding motif and an adjacent basic region. The ATF4 gene is mapped to chromosome 22. Unlike CREB, which activates transcription from CRE-containing promoters, CREB2 functions as a specific repressor of CRE-dependent transcription. The transcriptional repressor activity resides within the C-terminal leucine zipper and basic domain region of the CREB2 protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00371 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ATF4 using anti-ATF4 antibody (A00371).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF4 antigen affinity purified polyclonal antibody (Catalog # A00371) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF4 at approximately 50 kDa. The expected band size for ATF4 is at 39 kDa.
Specific Publications For Anti-ATF4 Antibody Picoband® (A00371)
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Customer Q&As
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15 Customer Q&As for Anti-ATF4 Antibody Picoband®
Question
We have observed staining in human lower esophagus. Are there any suggestions? Is anti-ATF4 antibody supposed to stain lower esophagus positively?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
According to literature lower esophagus does express ATF4. According to Uniprot.org, ATF4 is expressed in lower esophagus, fibroblast, leukemic t-cell, colon, lung, placenta urinary bladder, cervix carcinoma, among other tissues. Regarding which tissues have ATF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 20068231
Colon, Lung, Placenta, and Urinary bladder, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 1847461
Leukemic T-cell, Pubmed ID: 1534408
Boster Scientific Support
Answered: 2020-05-01
Question
I was wanting to use your anti-ATF4 antibody for WB for human colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human colon identification?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
You can see on the product datasheet, A00371 anti-ATF4 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-28
Question
Please see the WB image, lot number and protocol we used for colon using anti-ATF4 antibody A00371. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-11
Question
We are currently using anti-ATF4 antibody A00371 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The anti-ATF4 antibody (A00371) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-27
Question
Is a blocking peptide available for product anti-ATF4 antibody (A00371)?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
We do provide the blocking peptide for product anti-ATF4 antibody (A00371). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-25
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-ATF4 antibody A00371. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-10
Question
Will A00371 anti-ATF4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
You can see on the product datasheet, A00371 anti-ATF4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-06
Question
Is this A00371 anti-ATF4 antibody reactive to the isotypes of ATF4?
Verified Customer
Verified customer
Asked: 2018-12-24
Answer
The immunogen of A00371 anti-ATF4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human ATF4 (71-100aa SNNSKEDAFSGTDWMLEKMDLKEFDLDALL), different from the related mouse and rat sequences by five amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-24
Question
My boss were happy with the WB result of your anti-ATF4 antibody. However we have seen positive staining in fibroblast cytoplasm. using this antibody. Is that expected? Could you tell me where is ATF4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
Based on literature, fibroblast does express ATF4. Generally ATF4 expresses in cytoplasm. Regarding which tissues have ATF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 20068231
Colon, Lung, Placenta, and Urinary bladder, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 1847461
Leukemic T-cell, Pubmed ID: 1534408
Boster Scientific Support
Answered: 2018-06-01
Question
I see that the anti-ATF4 antibody A00371 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-08-08
Question
you antibody to test anti-ATF4 antibody A00371 on human colon for research purposes, then I may be interested in using anti-ATF4 antibody A00371 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Miller
Verified customer
Asked: 2017-04-10
Answer
The products we sell, including anti-ATF4 antibody A00371, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-04-10
Question
Can you help my question with product A00371, anti-ATF4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Carter
Verified customer
Asked: 2016-09-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00371 anti-ATF4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-09-21
Question
Do you have a BSA free version of anti-ATF4 antibody A00371 available?
M. Walker
Verified customer
Asked: 2015-09-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ATF4 antibody A00371 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-09-23
Question
you antibody using your anti-ATF4 antibody for mrna transcription by rna polymerase ii studies. Has this antibody been tested with western blotting on u2os whole cell lysates? We would like to see some validation images before ordering.
J. Baker
Verified customer
Asked: 2015-06-04
Answer
Thanks for your inquiry. This A00371 anti-ATF4 antibody is validated on u2os whole cell lysates, sw620 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-06-04
Question
Will anti-ATF4 antibody A00371 work for WB with colon?
C. Parker
Verified customer
Asked: 2014-06-16
Answer
According to the expression profile of colon, ATF4 is highly expressed in colon. So, it is likely that anti-ATF4 antibody A00371 will work for WB with colon.
Boster Scientific Support
Answered: 2014-06-16
