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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Boster Bio Anti-ATF4 Antibody catalog # PA1537. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1537)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of human ATF4 (332-351aa QYLKDLIEEVRKARGKKRVP), identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PA1537 is reactive to ATF4 in Human, Mouse, Rat
PA1537 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
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Anti-ATF4 antibody, PA1537, Western blotting
All lanes: Anti ATF4 (PA1537) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Lane 3: CEM Whole Cell Lysate at 40ug
Lane 4: HUT Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
Gene/Protein Information For ATF4 (Source: Uniprot.Org, NCBI)
Cyclic AMP-dependent transcription factor ATF-4
activating transcription factor 4 (tax-responsive enhancer element B67); Activating transcription factor 4; ATF4; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2DNA-binding protein TAXREB67; cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; TAXREB67; TAXREB67CREB2cAMP response element-binding protein 2; Tax-responsive enhancer element-binding protein 67; TXREB ATF4 CREB-2, CREB2, TAXREB67, TXREB activating transcription factor 4 cyclic AMP-dependent transcription factor ATF-4|DNA-binding protein TAXREB67|cAMP response element-binding protein 2|cAMP-dependent transcription factor ATF-4|cAMP-responsive element-binding protein 2|cyclic AMP-responsive element-binding protein 2|tax-responsive enhancer element B67|tax-responsive enhancer element-binding protein 67*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ATF4, check out the ATF4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ATF4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PA1537 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
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