Anti-ATF4 Antibody

Boster Bio Anti-ATF4 Antibody catalog # PA1537. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA1537
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-ATF4 Antibody

See all ATF4 products

SKU/Catalog Number

PA1537

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ATF4 Antibody catalog # PA1537. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ATF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1537)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ATF4 (332-351aa QYLKDLIEEVRKARGKKRVP), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1537 is reactive to ATF4 in Human, Mouse, Rat

Applications

PA1537 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For ATF4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P18848

Gene ID

468

Gene Name

ATF4

Full Name

Cyclic AMP-dependent transcription factor ATF-4

Weight

38.59kDa

Superfamily

bZIP family

Alternative Names

activating transcription factor 4 (tax-responsive enhancer element B67); Activating transcription factor 4; ATF4; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2DNA-binding protein TAXREB67; cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; TAXREB67; TAXREB67CREB2cAMP response element-binding protein 2; Tax-responsive enhancer element-binding protein 67; TXREB ATF4 CREB-2, CREB2, TAXREB67, TXREB activating transcription factor 4 cyclic AMP-dependent transcription factor ATF-4|DNA-binding protein TAXREB67|cAMP response element-binding protein 2|cAMP-dependent transcription factor ATF-4|cAMP-responsive element-binding protein 2|cyclic AMP-responsive element-binding protein 2|tax-responsive enhancer element B67|tax-responsive enhancer element-binding protein 67

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ATF4, check out the ATF4 Infographic

ATF4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ATF4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1537 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

MARVELD1 Inhibits Nonsense-Mediated RNA Decay by Repressing Serine Phosphorylation of UPF1

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4 Customer Q&As for Anti-ATF4 Antibody

Question

We have seen staining in mouse leukemic t-cell. Are there any suggestions? Is anti-ATF4 antibody supposed to stain leukemic t-cell positively?

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

Based on literature leukemic t-cell does express ATF4. Based on Uniprot.org, ATF4 is expressed in lower esophagus, fibroblast, leukemic t-cell, colon, lung, placenta urinary bladder, cervix carcinoma, among other tissues. Regarding which tissues have ATF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 20068231
Colon, Lung, Placenta, and Urinary bladder, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 1847461
Leukemic T-cell, Pubmed ID: 1534408

Boster Scientific Support

Answered: 2020-04-02

Question

Our lab were happy with the WB result of your anti-ATF4 antibody. However we have been able to see positive staining in placenta urinary bladder cytoplasm. using this antibody. Is that expected? Could you tell me where is ATF4 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

Based on literature, placenta urinary bladder does express ATF4. Generally ATF4 expresses in cytoplasm. Regarding which tissues have ATF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 20068231
Colon, Lung, Placenta, and Urinary bladder, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 1847461
Leukemic T-cell, Pubmed ID: 1534408

Boster Scientific Support

Answered: 2019-07-22

Question

My lab would like using your anti-ATF4 antibody for cellular calcium ion homeostasis studies. Has this antibody been tested with western blotting on a431 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-03-20

Answer

Thanks for your inquiry. This PA1537 anti-ATF4 antibody is validated on a431 whole cell lysate, raji whole cell lysate, cem whole cell lysate, hut whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-03-20

Question

We are currently using anti-ATF4 antibody PA1537 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

M. Li

Verified customer

Asked: 2017-11-09

Answer

The anti-ATF4 antibody (PA1537) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-11-09

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PA1537
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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