Anti-ATG16L Antibody

Product Name

Anti-ATG16L Antibody

View all ATG16L1 Antibodies

SKU/Catalog Number

A00526-2

Size

80 µl

Form

Liquid

Description

Boster Bio Anti-ATG16L Antibody (Catalog # A00526-2). Tested in IF, WB, IHC-P application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-ATG16L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00526-2)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from human ATG16L.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00526-2 is reactive to ATG16L1 in Human, Mouse

Applications

A00526-2 is guaranteed for IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

68.265kDa

Background of ATG16L1

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:1000
IHC-P: 1:10-1:50

Validation Images & Assay Conditions

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Immunofluorescent analysis of U251 cells, using ATG16L Antibody. U251 cells (right) were treated with Chloroquine (50 μM,16h). A00526-2 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

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Cos7, HEK293, MEF, and Hela cells, left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.

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Anti-ATG16L Antibody at 1:1000 dilution + Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 68 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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APG16L Antibody (Cat. #A00526-2) western blot analysis in NCI-H460 cell line lysates (35ug/lane). This demonstrates the APG16L antibody detected the APG16L protein (arrow).

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Western blot of APG16L (L92) Pab (Cat. #A00526-2) in mouse brain tissue lysate.

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Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Gene/Protein Information For ATG16L1 (Source: Uniprot.org, NCBI)

Alternative Names

APG16 autophagy 16-like (S. cerevisiae); APG16L beta; APG16LFLJ10828; APG16-like 1; ATG16 autophagy related 16-like (S. cerevisiae); ATG16 autophagy related 16-like 1 (S. cerevisiae); ATG16A; ATG16L; autophagy-related protein 16-1; FLJ00045; FLJ10035; FLJ22677; IBD10; WD repeat domain 30; WDR30 ATG16L1 APG16L, ATG16A, ATG16L, IBD10, WDR30 autophagy related 16 like 1 autophagy-related protein 16-1|APG16L beta|ATG16 autophagy related 16-like 1|WD repeat domain 30

For more info on ATG16L1, check out the ATG16L1 Infographic

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In this infographic, you will see the following information for ATG16L1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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