Mouse IL-1RA/IL1RN ELISA Kit PicoKine™

Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il1rn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0783
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse IL-1RA/IL1RN ELISA Kit PicoKine™

See all IL-1ra/IL-1F3/IL1RN products

SKU/Catalog Number

EK0783

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il1rn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0783)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R27-Q178

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0783 is reactive to Il1rn in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0783 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0783-CAP Anti-Mouse Il1rn Pre-coated 96-well strip microplate 1
EK0783-ST Mouse Il1rn Standard 2 vials, 10 ng/tube
EK0783-DA Mouse Il1rn Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0570.1190.1520.2520.4610.7921.3262.153

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-1ra/IL-1F3/IL1RN, Dilution ratio of 1:5, concentration in serum is around 4ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)995861056925701109
Standard deviation4.9536.9147.526.2542.1864.32
CV (%)5%7.4%4.5%6.8%7.4%5.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-1ra/IL-1F3/IL1RN in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 199899895953.894%
Sample 258655552354555222.664.1%
Sample 31056107011571068108740.333.7%
*number of samples for each test n=16.

Gene/Protein Information For Il1rn (Source: Uniprot.Org, NCBI)

Uniprot ID

P25085

Gene ID

16181

Gene Name

Il1rn

Full Name

Interleukin-1 receptor antagonist protein

Weight

20.274kDa

Superfamily

IL-1 family

Alternative Names

DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4 Il1rn|F630041P17Rik, IL, IL-1ra|interleukin 1 receptor antagonist|interleukin-1 receptor antagonist protein|IL-1RN|IL1 inhibitor|IRAP

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il1rn, check out the Il1rn Infographic

Il1rn infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il1rn: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Mouse IL-1RA/IL1RN ELISA Kit PicoKine™

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring IL-1ra using the EK0783 Mouse IL-1ra Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2019-12-11

Question

Q: can I use citrate plasma as samples in Mouse IL-1ra Picokine® ELISA Kit (Catalog # EK0783)?

Verified Customer

Verified customer

Asked: 2018-05-20

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of IL-1ra causing degradation of its protein structure. IL-1ra may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-1ra ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-05-20

Question

Q: if the enzyme conjugated IL-1ra antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1ra antigen?

Verified Customer

Verified customer

Asked: 2017-12-29

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-12-29

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2014-11-21

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2014-11-21

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