Overview
| Product Name |
Mouse IL-1RA/IL1RN ELISA Kit PicoKine™
See more IL-1ra/IL-1F3/IL1RN products |
| Catalog# |
EK0783 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il1rn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product |
Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0783)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Mouse |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10 pg/ml |
| Assay Range |
62.5 pg/ml - 4,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: E.coli; Immunogen sequence: R27-Q178 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Il1rn (Source: Uniprot.org, NCBI)
| Uniprot Id | P25085 |
|---|
| NCBI Gene Id | 16181 |
|---|
| Species Of This Entry | Mouse |
|---|
| Gene Name | Il1rn |
|---|
| Protein Name | Interleukin-1 receptor antagonist protein |
|---|
| Superfamily | IL-1 family |
|---|
| Alternative Names | IL-1ra/IL-1F3/IL1RN|DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4 |
|---|
| Molecular Weight | 20274 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il1rn, check out the Il1rn Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Il1rn: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Il1rn infographic
Kit Components And Assay QC Data Details Of Mouse IL-1RA/IL1RN ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0783-CAP |
Anti-Mouse Il1rn Pre-coated 96-well strip microplate | 1 |
| EK0783-ST | Mouse Il1rn Standard | 2 vials, 10 ng/tube |
| EK0783-DA | Mouse Il1rn Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0783 Mouse IL-1RA/IL1RN ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.057 | 0.119 | 0.152 | 0.252 | 0.461 | 0.792 | 1.326 | 2.153 |
We measured random samples of Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 99 | 586 | 1056 | 92 | 570 | 1109 |
| Standard deviation | 4.95 | 36.91 | 47.52 | 6.25 | 42.18 | 64.32 |
| CV (%) | 5% | 7.4% | 4.5% | 6.8% | 7.4% | 5.8% |
We measured the performance consistency of Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 99 | 89 | 98 | 95 | 95 | 3.89 | 4% |
| Sample 2 | 586 | 555 | 523 | 545 | 552 | 22.66 | 4.1% |
| Sample 3 | 1056 | 1070 | 1157 | 1068 | 1087 | 40.33 | 3.7% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
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Questions and answers from customers related to EK0783 Mouse IL-1RA/IL1RN ELISA Kit PicoKine™
4 Related Questions
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring IL-1ra using the EK0783 Mouse IL-1ra Picokine® ELISA Kit?
Verified Customer
Asked: 2019-12-11
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2019-12-11
Question
Q: can I use citrate plasma as samples in Mouse IL-1ra Picokine® ELISA Kit (Catalog # EK0783)?
Verified Customer
Asked: 2018-05-20
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of IL-1ra causing degradation of its protein structure. IL-1ra may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-1ra ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-05-20
Question
Q: if the enzyme conjugated IL-1ra antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1ra antigen?
Verified Customer
Asked: 2017-12-29
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-12-29
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2014-11-21
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-11-21
