Overview
| Product Name |
Mouse IL-1RA/IL1RN ELISA Kit PicoKine™
See all IL-1ra/IL-1F3/IL1RN primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0783 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Mouse Il 1Ra/IL1RN with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il 1Ra/IL1RN tissue specificity: |
| Cite This Product |
Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0783)
|
Product Specs
| Reactivity/Species |
Mouse |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10pg/ml |
| Assay Range |
62.5pg/ml-4000pg/ml |
| Immunogen/Standard |
Expression system for standard: E.coli; Immunogen sequence: R27-Q178 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For Il1rn (Source: Uniprot.org, NCBI)
| Uniprot Id | P25085 |
|---|
| NCBI Gene Id | 16181 |
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| Species Of This Entry | Mouse |
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| Gene Name | Il1rn |
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| Protein Name | Interleukin-1 receptor antagonist protein |
|---|
| Superfamily | IL-1 family |
|---|
| Alternative Names | IL-1ra/IL-1F3/IL1RN|DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4 |
|---|
| Molecular Weight | 20274 |
|---|
| Post Tranlational Modifications | Phosphorylation; Cleavage; Glycosylation; Reduction; Oxidation; Nitration; Methylation; Formylation; Carboxylation; Biotinylation; Citrullination; Dephosphorylation |
|---|
| Gene Location | 2 A3|2 16.36 cM, on Chromasome 2, gene sequence: |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Il1rn (Source: Uniprot.org, NCBI)
| Protein Function | Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. |
|---|
| Related Diseases | Inflammation; Arthritis; Rheumatoid Arthritis; Variable Number Tandem Repeat; Malignant Neoplasms; Inflammatory Response; Systemic Infection; Infective Disorder; Autoimmune Reaction; Neoplasms; Tissue Adhesions; Degenerative Polyarthritis; Autoimmune Diseases; Ulcer; Pain; Diabetes Mellitus; Colitis; Leukemia; Fibrosis; Inflammatory Disorder |
|---|
| Related Pathways | Pathogenesis; Secretion; Inflammatory Response; Cytokine Production; Immune Response; Bone Resorption; Cell Proliferation; Localization; Cell Adhesion; Translation; Cell Death; Cell Activation; Cell Growth; Cytokine Secretion; Acute-phase Response; Coagulation; Excretion; Aging; Hypersensitivity; Menopause |
|---|
| Background | The interleukin-1 receptor antagonist(IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization. IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha(IL1A) and interleukin 1, beta(IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. |
|---|
| Scientific References | PMID: 1828262 by Zahedi K., et al. Mouse IL-1 receptor antagonist protein. Molecular characterization, gene mapping, and expression of mRNA in vitro and in vivo.
PMID: 1830498 by Matsushime H., et al. Cloning and expression of murine interleukin-1 receptor antagonist in macrophages stimulated by colony-stimulating factor 1. |
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Kit Components And Assay QC Data Details Of Mouse IL-1RA/IL1RN ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0783-CAP | 96-well plate precoated with anti-Mouse IL-1ra/IL-1F3/IL1RN antibody | 1 |
| EK0783-ST | lyophilized recombinant Mouse IL-1ra/IL-1F3/IL1RN standard | 10ng/tube |
| EK0783-DA | biotinylated anti-Mouse IL-1ra/IL-1F3/IL1RN antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0783 Mouse IL-1RA/IL1RN ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.057 | 0.119 | 0.152 | 0.252 | 0.461 | 0.792 | 1.326 | 2.153 |
We measured random samples of Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 99 | 586 | 1056 | 92 | 570 | 1109 |
| Standard deviation | 4.95 | 36.91 | 47.52 | 6.25 | 42.18 | 64.32 |
| CV(%) | 5% | 7.4% | 4.5% | 6.8% | 7.4% | 5.8% |
We measured the performance consistency of Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 99 | 89 | 98 | 95 | 95 | 3.89 | 4% |
| Sample 2 | 586 | 555 | 523 | 545 | 552 | 22.66 | 4.1% |
| Sample 3 | 1056 | 1070 | 1157 | 1068 | 1087 | 40.33 | 3.7% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all IL-1ra/IL-1F3/IL1RN primary antibodies,
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