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Product Info Summary
| SKU: | EK0783 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Mouse IL-1RA/IL1RN ELISA Kit PicoKine™
See all IL-1ra/IL-1F3/IL1RN products
SKU/Catalog Number
EK0783
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il1rn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0783)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: R27-Q178
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0783 is reactive to Il1rn in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0783 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0783-CAP | Anti-Mouse Il1rn Pre-coated 96-well strip microplate | 1 |
| EK0783-ST | Mouse Il1rn Standard | 2 vials, 10 ng/tube |
| EK0783-DA | Mouse Il1rn Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.057 | 0.119 | 0.152 | 0.252 | 0.461 | 0.792 | 1.326 | 2.153 |
Data Example Images
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Mouse IL-1RA/IL1RN PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-1ra/IL-1F3/IL1RN, Dilution ratio of 1:5, concentration in serum is around 4ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 99 | 586 | 1056 | 92 | 570 | 1109 |
| Standard deviation | 4.95 | 36.91 | 47.52 | 6.25 | 42.18 | 64.32 |
| CV (%) | 5% | 7.4% | 4.5% | 6.8% | 7.4% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-1ra/IL-1F3/IL1RN in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 99 | 89 | 98 | 95 | 95 | 3.89 | 4% |
| Sample 2 | 586 | 555 | 523 | 545 | 552 | 22.66 | 4.1% |
| Sample 3 | 1056 | 1070 | 1157 | 1068 | 1087 | 40.33 | 3.7% |
Protein Target Info & Infographic
Gene/Protein Information For Il1rn (Source: Uniprot.Org, NCBI)
Uniprot ID
P25085
Gene ID
16181
Gene Name
Il1rn
Full Name
Interleukin-1 receptor antagonist protein
Weight
20.274kDa
Superfamily
IL-1 family
Alternative Names
DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4 Il1rn|F630041P17Rik, IL, IL-1ra|interleukin 1 receptor antagonist|interleukin-1 receptor antagonist protein|IL-1RN|IL1 inhibitor|IRAP
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il1rn, check out the Il1rn Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il1rn: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Mouse IL-1RA/IL1RN ELISA Kit PicoKine™ (EK0783)
No publications found for EK0783
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Recommended Resources
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Mouse IL-1RA/IL1RN ELISA Kit PicoKine™
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring IL-1ra using the EK0783 Mouse IL-1ra Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2019-12-11
Question
Q: can I use citrate plasma as samples in Mouse IL-1ra Picokine® ELISA Kit (Catalog # EK0783)?
Verified Customer
Verified customer
Asked: 2018-05-20
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of IL-1ra causing degradation of its protein structure. IL-1ra may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-1ra ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-05-20
Question
Q: if the enzyme conjugated IL-1ra antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1ra antigen?
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-12-29
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2014-11-21
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-11-21
