Product Info Summary
SKU: | A00762Y8 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-AURKB (Y8) Antibody
SKU/Catalog Number
A00762Y8
Size
100ul
Form
Liquid
Description
Boster Bio Anti-AURKB (Y8) Antibody catalog # A00762Y8. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AURKB (Y8) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00762Y8)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human ARK-2 around the phosphorylation site of Y12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00762Y8 is reactive to AURKB in Human, Mouse, Rat
Applications
A00762Y8 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
39.311kDa
Background of Aurora B
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
Ana V. Miletic, J. Biol. Chem., Dec 2006; 281: 38257 - 38265.
Christopher C. Stebbins, Mol. Cell. Biol., Sep 2003; 23: 6291 - 6299.
Vered Schapira, Cancer Res., Jun 2006; 66: 6183 - 6191.
Maya Groysman, J. Biol. Chem., Dec 2002; 277: 50121 - 50130.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of AURKB using Anti-AURKB (Y8) Antibody (A00762Y8).
Immunohistochemistry (IHC) analyzes of AURKB (Y8) pAb in paraffin-embedded liver cancer tissue at 1:100.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-AURKB (Y8) Antibody A00762Y8
Western blot (WB) analysis of AURKB (Y8) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate(40ug)
Lane2:AML-12 whole cell lysate(40ug)
Lane3:PC12 whole cell lysate(40ug)
Lane4:Jurkat whole cell lysate(40ug)
Lane5:THP-1 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For AURKB (Source: Uniprot.org, NCBI)
Gene Name
AURKB
Full Name
Aurora kinase B
Weight
39.311kDa
Superfamily
protein kinase superfamily
Alternative Names
Aik2; AIK2EC 2.7.11.1; AIM1; AIM-1; AIM1Aik2; AIM-1STK-1; ARK2; ARK2ARK-2; AurB; AURKB; aurkb-sv2; Aurora- and IPL1-like midbody-associated protein 1; Aurora B; aurora kinase BSerine/threonine-protein kinase aurora-B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora/IPL1-related kinase 2; aurora-1; aurora-B; Aurora-related kinase 2; EC 2.7.11; IPL1; serine/threonine kinase 12; serine/threonine-protein kinase 12; STK12; STK12aurkb-sv1; STK5 AURKB AIK2, AIM-1, AIM1, ARK-2, ARK2, AurB, IPL1, PPP1R48, STK-1, STK12, STK5, aurkb-sv1, aurkb-sv2 aurora kinase B aurora kinase B|aurora kinase B-Sv1|aurora kinase B-Sv2|aurora- and Ipl1-like midbody-associated protein 1|aurora-1|aurora-B|aurora-related kinase 2|aurora/IPL1-related kinase 2|protein phosphatase 1, regulatory subunit 48|serine/threonine kinase 12|serine/threonine-protein kinase 12|serine/threonine-protein kinase 5|serine/threonine-protein kinase aurora-B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AURKB, check out the AURKB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AURKB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AURKB (Y8) Antibody (A00762Y8)
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No publications found for A00762Y8
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