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Product Info Summary
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Anti-RPGR Antibody Picoband™
Boster Bio Anti-RPGR Antibody Picoband™ catalog # A01522. Tested in ELISA, WB applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPGR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01522)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human RPGR recombinant protein (Position: A13-F338).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A01522 is reactive to RPGR in Rat
A01522 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RPGR using anti-RPGR antibody (A01522).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPGR antigen affinity purified polyclonal antibody (Catalog # A01522) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPGR at approximately 113KD. The expected band size for RPGR is at 113KD.
Gene/Protein Information For RPGR (Source: Uniprot.Org, NCBI)
X-linked retinitis pigmentosa GTPase regulator
COD1; cone dystrophy 1 (X-linked); CORDX1RP3retinitis pigmentosa 15; CRD; orf15; PCDX; retinitis pigmentosa 3 GTPase regulator; retinitis pigmentosa GTPase regulator; RP15; X-linked retinitis pigmentosa GTPase regulator; XLRP3 RPGR COD1, CORDX1, CRD, PCDX, RP15, RP3, XLRP3, orf15 retinitis pigmentosa GTPase regulator X-linked retinitis pigmentosa GTPase regulator|retinitis pigmentosa 15|retinitis pigmentosa 3 GTPase regulator*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on RPGR, check out the RPGR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RPGR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for A01522
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