Anti-RPGR Antibody Picoband™

Product Name

Anti-RPGR Antibody Picoband™

View all RPGR Antibodies

SKU/Catalog Number

A01522

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RPGR Antibody Picoband™ catalog # A01522. Tested in ELISA, WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RPGR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01522)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human RPGR recombinant protein (Position: A13-F338).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01522 is reactive to RPGR in Rat

Applications

A01522 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

113 kDa

Calculated molecular weight

113.387kDa

Background of RPGR

X-linked retinitis pigmentosa GTPase regulator is a GTPase-binding protein that in humans is encoded by the RPGR gene. This gene encodes a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of RPGR using anti-RPGR antibody (A01522).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPGR antigen affinity purified polyclonal antibody (Catalog # A01522) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPGR at approximately 113KD. The expected band size for RPGR is at 113KD.

Gene/Protein Information For RPGR (Source: Uniprot.org, NCBI)

Alternative Names

COD1; cone dystrophy 1 (X-linked); CORDX1RP3retinitis pigmentosa 15; CRD; orf15; PCDX; retinitis pigmentosa 3 GTPase regulator; retinitis pigmentosa GTPase regulator; RP15; X-linked retinitis pigmentosa GTPase regulator; XLRP3 RPGR COD1, CORDX1, CRD, PCDX, RP15, RP3, XLRP3, orf15 retinitis pigmentosa GTPase regulator X-linked retinitis pigmentosa GTPase regulator|retinitis pigmentosa 15|retinitis pigmentosa 3 GTPase regulator

For more info on RPGR, check out the RPGR Infographic

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In this infographic, you will see the following information for RPGR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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