Anti-Aurora B/AURKB Antibody Picoband®

Product Name

Anti-Aurora B/AURKB Antibody Picoband®

View all Aurkb Antibodies

SKU/Catalog Number

PA1806
BA3799-2 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Aurora B/AURKB Antibody catalog # PA1806. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aurora B/AURKB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1806)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Aurora B, different from the related rat sequence by two anino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1806 is reactive to Aurkb in Human, Mouse, Rat

Observed Molecular Weight

39 kDa

Calculated molecular weight

39.4 kDa

Background of Aurkb

AURKB (aurora kinase B) also called Aik2, AIM-1, ARK2, AurB, STK12, IPL1, PPP1R48 or STK5, localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. Cell cycle and Northern blot analyses showed that STK12 is expressed in the S phase and persistently thereafter. Western blot analysis indicated that STK12 is localized in the midbodies during anaphase. Northern blot analysis detected strong expression of a 1.5-kb STK12 transcript in thymus, with weaker expression in small intestine, testis, colon, spleen, and brain. The AURKB gene is mapped on 17p13.1. Examination of the role of both kinases in the phosphorylation of CENPA revealed that the reaction is mediated sequentially by AURKA and AURKB in early mitosis. EB1 overexpression enhanced AURKB kinase activity, and knockdown of EB1 with small interfering RNA impaired AURKB activity. EB1 protected AURKB from dephosphorylation/inactivation by protein phosphatase-2A by blocking binding of PP2A to AURKB. 

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1806 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: human 293T whole cell, human Jurkat whole cell, human U251 whole cell, human A549 whole cell, rat spleen tissue, mouse spleen tissue

Validation Images & Assay Conditions

See full target information Aurkb

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