Anti-Aurora B/AURKB Antibody

Aurora B antibody

Boster Bio Anti-Aurora B/AURKB Antibody catalog # PA1806. Tested in WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: PA1806
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Aurora B/AURKB Antibody

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SKU/Catalog Number



100 μg/vial




Boster Bio Anti-Aurora B/AURKB Antibody catalog # PA1806. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aurora B/AURKB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1806)




Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.




Rabbit IgG


A synthetic peptide corresponding to a sequence at the N-terminus of mouse Aurora B (72-87aa QNKQPFTIDNFEIGRP), different from the related rat sequence by two anino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.


No cross-reactivity with other proteins

Reactive Species

PA1806 is reactive to Aurkb in Mouse, Rat


PA1806 is guaranteed for WB Boster Guarantee

Background of Aurora B

AURKB (aurora kinase B) also called Aik2, AIM-1, ARK2, AurB, STK12, IPL1, PPP1R48 or STK5, localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. Cell cycle and Northern blot analyses showed that STK12 is expressed in the S phase and persistently thereafter. Western blot analysis indicated that STK12 is localized in the midbodies during anaphase. Northern blot analysis detected strong expression of a 1.5-kb STK12 transcript in thymus, with weaker expression in small intestine, testis, colon, spleen, and brain. The AURKB gene is mapped on 17p13.1. Examination of the role of both kinases in the phosphorylation of CENPA revealed that the reaction is mediated sequentially by AURKA and AURKB in early mitosis. EB1 overexpression enhanced AURKB kinase activity, and knockdown of EB1 with small interfering RNA impaired AURKB activity. EB1 protected AURKB from dephosphorylation/inactivation by protein phosphatase-2A by blocking binding of PP2A to AURKB. 

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Aurkb (Source:, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

Aurora kinase B




protein kinase superfamily

Alternative Names

Aik2; AIK2EC; AIM1; AIM-1; AIM1Aik2; AIM-1STK-1; ARK2; ARK2ARK-2; AurB; AURKB; aurkb-sv2; Aurora- and IPL1-like midbody-associated protein 1; Aurora B; aurora kinase BSerine/threonine-protein kinase aurora-B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora/IPL1-related kinase 2; aurora-1; aurora-B; Aurora-related kinase 2; EC 2.7.11; IPL1; serine/threonine kinase 12; serine/threonine-protein kinase 12; STK12; STK12aurkb-sv1; STK5 Aurkb|AI, AIM-1, AIRK2, AL022959, Aik2, Aim1, Ark2, AurB, I, IPL1, STK-, STK-1, Stk, Stk1, Stk12, Stk5|aurora kinase B|aurora kinase B|ARK-2|aurora 1|aurora B|aurora- and IPL1-like midbody-associated protein 1|aurora-related kinase 2|aurora/IPL1-related kinase 2|serine/threonine kinase 12|serine/threonine kinase 5|serine/threonine-protein kinase 12|serine/threonine-protein kinase 5|serine/threonine-protein kinase aurora-B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Aurkb, check out the Aurkb Infographic

Aurkb infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Aurkb: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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4 Customer Q&As for Anti-Aurora B/AURKB Antibody


We were well pleased with the WB result of your anti-Aurora B/AURKB antibody. However we have been able to see positive staining in lung nucleus. chromosome. chromosome using this antibody. Is that expected? Could you tell me where is AURKB supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-04


According to literature, lung does express AURKB. Generally AURKB expresses in nucleus. chromosome. chromosome, centromere. Regarding which tissues have AURKB expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, and Spleen, Pubmed ID: 9858806
Lung, Lymph, and Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-04


We want using your anti-Aurora B/AURKB antibody for histone h3-s28 phosphorylation studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-25


Thanks for your inquiry. This PA1806 anti-Aurora B/AURKB antibody is tested on rat liver tissue, tissue lysate, 22rv cell lysate, hela cell lysate, sw620 cell lysate. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-25


We have observed staining in mouse cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-Aurora B/AURKB antibody supposed to stain cervix carcinoma erythroleukemia positively?

Verified Customer

Verified customer

Asked: 2019-06-03


Based on literature cervix carcinoma erythroleukemia does express AURKB. Based on, AURKB is expressed in oocyte, liver spleen, lung, lymph muscle, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have AURKB expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, and Spleen, Pubmed ID: 9858806
Lung, Lymph, and Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-06-03


We are currently using anti-Aurora B/AURKB antibody PA1806 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on goat tissues as well?

V. Brown

Verified customer

Asked: 2013-01-10


The anti-Aurora B/AURKB antibody (PA1806) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-01-10



Total: $315


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