Anti-Aurora B/AURKB Antibody

Boster Bio Anti-Aurora B/AURKB Antibody catalog # PA1806. Tested in WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: PA1806
Size: 100μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Aurora B/AURKB Antibody

See all Aurora B products

SKU/Catalog Number

PA1806

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Aurora B/AURKB Antibody catalog # PA1806. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aurora B/AURKB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1806)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Aurora B (72-87aa QNKQPFTIDNFEIGRP), different from the related rat sequence by two anino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1806 is reactive to Aurkb in Mouse, Rat

Applications

PA1806 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Aurkb (Source: Uniprot.Org, NCBI)

Uniprot ID

O70126

Gene ID

20877

Gene Name

Aurkb

Full Name

Aurora kinase B

Weight

39.384kDa

Superfamily

protein kinase superfamily

Alternative Names

Aik2; AIK2EC 2.7.11.1; AIM1; AIM-1; AIM1Aik2; AIM-1STK-1; ARK2; ARK2ARK-2; AurB; AURKB; aurkb-sv2; Aurora- and IPL1-like midbody-associated protein 1; Aurora B; aurora kinase BSerine/threonine-protein kinase aurora-B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora/IPL1-related kinase 2; aurora-1; aurora-B; Aurora-related kinase 2; EC 2.7.11; IPL1; serine/threonine kinase 12; serine/threonine-protein kinase 12; STK12; STK12aurkb-sv1; STK5 Aurkb|AI, AIM-1, AIRK2, AL022959, Aik2, Aim1, Ark2, AurB, I, IPL1, STK-, STK-1, Stk, Stk1, Stk12, Stk5|aurora kinase B|aurora kinase B|ARK-2|aurora 1|aurora B|aurora- and IPL1-like midbody-associated protein 1|aurora-related kinase 2|aurora/IPL1-related kinase 2|serine/threonine kinase 12|serine/threonine kinase 5|serine/threonine-protein kinase 12|serine/threonine-protein kinase 5|serine/threonine-protein kinase aurora-B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Aurkb, check out the Aurkb Infographic

Aurkb infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Aurkb: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Anti-Aurora B/AURKB Antibody

Question

We were well pleased with the WB result of your anti-Aurora B/AURKB antibody. However we have been able to see positive staining in lung nucleus. chromosome. chromosome using this antibody. Is that expected? Could you tell me where is AURKB supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

According to literature, lung does express AURKB. Generally AURKB expresses in nucleus. chromosome. chromosome, centromere. Regarding which tissues have AURKB expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, and Spleen, Pubmed ID: 9858806
Lung, Lymph, and Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-04

Question

We want using your anti-Aurora B/AURKB antibody for histone h3-s28 phosphorylation studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

Thanks for your inquiry. This PA1806 anti-Aurora B/AURKB antibody is tested on rat liver tissue, tissue lysate, 22rv cell lysate, hela cell lysate, sw620 cell lysate. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-25

Question

We have observed staining in mouse cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-Aurora B/AURKB antibody supposed to stain cervix carcinoma erythroleukemia positively?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

Based on literature cervix carcinoma erythroleukemia does express AURKB. Based on Uniprot.org, AURKB is expressed in oocyte, liver spleen, lung, lymph muscle, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have AURKB expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, and Spleen, Pubmed ID: 9858806
Lung, Lymph, and Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-06-03

Question

We are currently using anti-Aurora B/AURKB antibody PA1806 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on goat tissues as well?

V. Brown

Verified customer

Asked: 2013-01-10

Answer

The anti-Aurora B/AURKB antibody (PA1806) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-01-10

Size

Total: $240

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PA1806
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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