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Product Info Summary
| SKU: | M00075-3 |
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| Size: | 100 µl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IF, IHC, WB |
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Product info
Product Name
Anti-B Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: OTI5A9]
SKU/Catalog Number
M00075-3
Size
100 µl
Description
Boster Bio BRAF mouse monoclonal antibody, clone OTI5A9 (formerly 5A9). Catalog# M00075-3. Tested in FC, IF, IHC, IP, WB. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-B Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: OTI5A9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00075-3)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5A9
Isotype
IgG1
Immunogen
Full length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00075-3 is reactive to BRAF in Human, Monkey, Mouse, Rat
Reconstitution
Observed Molecular Weight
85 kDa
Calculated molecular weight
84.437kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00075-3 is guaranteed for Flow Cytometry, IP, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells transfected with either BRAF (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BRAF antibody (M00075-3)
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Anti-BRAF mouse monoclonal antibody (M00075-3) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF.
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
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Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (M00075-3)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and BRAF-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-BRAF monoclonal antibody M00075-3 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
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Western blot analysis of extracts (35ug) from 4 tissue lysates by using anti-BRAF monoclonal antibody (1:500).
Protein Target Info & Infographic
Gene/Protein Information For BRAF (Source: Uniprot.org, NCBI)
Gene Name
BRAF
Full Name
Serine/threonine-protein kinase B-raf
Weight
84.437kDa
Superfamily
protein kinase superfamily
Alternative Names
94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRaf; B-Raf; BRAF1; B-RAF1; BRAF1MGC126806; EC 2.7.11.1; MGC138284; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; Proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B1FLJ95109 BRAF B-RAF1, B-raf1, NS7, RAFB1, BRAF B-Raf proto-oncogene, serine/threonine kinase serine/threonine-protein kinase B-raf|94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|B-Raf serine/threonine-protein|murine sarcoma viral (v-raf) oncogene homolog B1|proto-oncogene B-Raf|v-raf murine sarcoma viral oncogene homolog B|v-raf murine sarcoma viral oncogene homolog B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRAF, check out the BRAF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRAF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: OTI5A9] (M00075-3)
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