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- Table of Contents
KRT19 antibody (PA1335) performs well in IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PA1335).
Cytokeratin 19 was detected in a paraffin-embedded section of Human Oesophagus Squama Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 19 Antibody (PA1335) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Cytokeratin 19 was detected in a paraffin-embedded section of Human Oesophagus Squama Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 19 Antibody (PA1335) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-Cytokeratin 19/KRT19 Antibody Picoband®
KRT19 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC, ICC, WB |
Tested Applications
KRT19 antibody was positively detected by WB in:
human MCF-7 whole cell, human MDA-MB-453 whole cell, human placenta tissue, human RT4 whole cell, human T-47D whole cell, human A431 whole cell, human HL-60 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
KRT19 antibody was positively detected by IHC in:
Human Oesophagus Squama Cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
KRT19 antibody was positively detected by IHC-F in:
human placenta tissue
KRT19 antibody was positively detected by ICC in:
MCF-7 cell
Recommend Dilution
Application
Dilution
Species
Immunocytochemistry
0.5-1μg/ml
Human, -
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human, -
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Cytokeratin 19/KRT19 Antibody Picoband®
View all KRT19 Antibodies
View all KRT19 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 19.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated Molecular Weight
44.1 kDa
Background of KRT19
Keratin, type I cytoskeletal 19 is a protein that in humans is encoded by the KRT19 gene.The protein encoded by this gene is a member of the keratin family. It is specifically expressed in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.Keratin 19 is often used together with keratin 8 and keratin 18 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.
Cite This Product
Anti-Cytokeratin 19/KRT19 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1335)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
93%
High Homology
Rat
86%
Moderate Homology
Rabbit
86%
Moderate Homology
Dog
86%
Moderate Homology
Cow
57%
Low homology
Zebrafish
57%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for KRT19 antibody
Immunohistochemistry (IHC) protocol for KRT19 antibody
Immunocytochemistry (ICC) protocol for KRT19 antibody
Publications
Application:
Species:
Wu T,Tang C,Chen Y,Yong X,Liu Z,Jiang L,Zeng Q,Tao R.Regulatory effect of 17β-estradiol on the expression of β-defensin-2 and proinflammatory cytokines in human oral epithelial cells.J Oral Pathol Med.2020 Apr;49(4):365-372.doi:10.1111/jop.13016.Epub 2020
Species: Human
PMID: 32176389
Selective tropism of liver stem cells to hepatocellular carcinoma in vivo.
PMID: 29246405
Quan, J., Du, Q., Hou, Y., Wang, Z., & Zhang, J. (2017). Utilization of E-cadherin by monocytes from tumour cells plays key roles in the progression of bone invasion by oral squamous cell carcinoma. Oncology Reports, 38(2), 850-858. Advance online...
PMID: 28656299
Yin D, Tian L, Ye Y, Li K, Wang J, Cheng P, Chen A, Guo F, Huang H. Int J Mol Med. 2012 Apr;29(4):587-92. Doi: 10.3892/Ijmm.2011.871. Epub 2011 Dec 29. Nanog And ??-Catenin: A New Convergence Point In Epsc Proliferation And Differentiation.
PMID: 22211239
Lu J, Gu Yp, Xu X, Liu Ml, Xie P, Song Hp. World J Gastroenterol. 2005 Jun 14;11(22):3426-30. Adult Islets Cultured In Collagen Gel Transdifferentiate Into Duct-Like Cells.
PMID: 15948249
Li M, Zhang B, Zhang Z, Liu X, Qi X, Zhao J, Jiang Y, Zhai H, Ji Y, Luo D. Biomed Res Int. 2014;2014:981261. Doi: 10.1155/2014/981261. Epub 2014 May 22. Stem Cell-Like Circulating Tumor Cells Indicate Poor Prognosis In Gastric Cancer.
PMID: 24963492
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Product Promise
We promise Anti-Cytokeratin 19/KRT19 Antibody Picoband® performs excellently in relevant applications for IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1335
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026