Anti-Cytokeratin 19/KRT19 Antibody
Rabbit IgG polyclonal antibody for Keratin, type I cytoskeletal 19(KRT19) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. Cited in 5 publication(s).
Anti-Cytokeratin 19/KRT19 Antibody Info At A Glance
|Applications:||IHC, ICC, WB|
|Product Name||Anti-Cytokeratin 19/KRT19 Antibody
See all Cytokeratin 19 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Cytokeratin 19/KRT19 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Cytokeratin 19/KRT19 information: Molecular Weight: 44106 MW; Tissue Specificity: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.|
|Cite This Product||Anti-Cytokeratin 19/KRT19 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1335)|
|Similar Products From
|Anti-Cytokeratin 19/KRT19 Antibody may replace the following items: sc 25724|sc 33110|sc 33111|sc 33119|sc 374192|sc 374386|sc 376126|sc 53003|sc 53257|sc 53258|sc 56371|sc 6278.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 19(379-392aa YRSLLEGQEDHYNN).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For KRT19 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3880|
|Species Of This Entry||Human|
|Protein Name||Keratin, type I cytoskeletal 19|
|Superfamily||intermediate filament family|
|Alternative Names||Cytokeratin 19|40-kDa keratin intermediate filament; CK19; CK-19; Cytokeratin 19; EndoC; K19; K1CS; keratin 19; keratin, type I cytoskeletal 19; keratin, type I, 40-kd; keratin-19; Krt19; MGC15366|
|Post Tranlational Modifications||Methylation; Demethylation|
|Gene Location||17q21.2, on Chromasome 17, gene sequence: NC_000017.11 (41523617..41528308, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For KRT19 (Source: Uniprot.org, NCBI)
|Protein Function||Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.|
|Tissue Specificity||Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.|
|Interacting Proteins||C1orf109; CARD9; COPS4; DMD; EIF4E2; EIF4ENIF1; EXOC8; FAM124B; GFAP; HAUS1; HGS; IKZF3; KIFC3; KRT2; KRT6B; POLR1C; PPP1R18; TBC1D7; TGM2|
|Research Areas||Cancer; Cell Biology; Cellular Markers; Stem Cell Markers|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Malignant Neoplasm Of Breast; Mammary Neoplasms; Neoplasm Metastasis; Tissue Adhesions; Adenocarcinoma; Kidney Neoplasm; Liver Carcinoma; Malignant Paraganglionic Neoplasm; Thyroid Neoplasm; Liver Diseases; Renal Cell Carcinoma; Liver Neoplasms; Malignant Neoplasm Of Lung; Papillary Thyroid Carcinoma; Cell Invasion; Malignant Neoplasm Of Thyroid; Adenoma|
|Related Pathways||Methylation; Cell Adhesion; Cell Proliferation; Dna Methylation; Pathogenesis; Transport; Drug Resistance; Rna Interference; Cell Cycle; Cell Communication; Localization; Regeneration; Cell Migration; Gene Silencing; Aging; Immune Response; Inflammatory Response; Blastocyst Formation; Cytoskeleton Organization; Reverse Transcription|
|Background||Keratin, type I cytoskeletal 19 is a protein that in humans is encoded by the KRT19 gene.The protein encoded by this gene is a member of the keratin family. It is specifically expressed in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.Keratin 19 is often used together with keratin 8 and keratin 18 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.|
|Scientific References||PMID: 2447559 by Stasiak P.C., et al. Sequence of cDNA coding for human keratin 19.|
PMID: 2468493 by Bader B.L., et al. Low level expression of cytokeratins 8, 18 and 19 in vascular smooth muscle cells of human umbilical cord and in cultured cells derived therefrom, with an analysis of the chromosomal locus containing the cytokeratin 19 gene.
Our Boster Quality Guarantee for Anti-Cytokeratin 19/KRT19 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
IHC(P): Human Oesophagus Squama Cancer Tissue
ICC: MCF-7 Cell
Cytokeratin 19 was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Cytokeratin 19 Antibody (PA1335) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 5
|Li M, Zhang B, Zhang Z, Liu X, Qi X, Zhao J, Jiang Y, Zhai H, Ji Y, Luo D. Biomed Res Int. 2014;2014:981261. Doi: 10.1155/2014/981261. Epub 2014 May 22. Stem Cell-Like Circulating Tumor Cells Indicate Poor Prognosis In Gastric Cancer.||PubMed ID 24963492|
|Lu J, Gu Yp, Xu X, Liu Ml, Xie P, Song Hp. World J Gastroenterol. 2005 Jun 14;11(22):3426-30. Adult Islets Cultured In Collagen Gel Transdifferentiate Into Duct-Like Cells.||PubMed ID 15948249|
|Yin D, Tian L, Ye Y, Li K, Wang J, Cheng P, Chen A, Guo F, Huang H. Int J Mol Med. 2012 Apr;29(4):587-92. Doi: 10.3892/Ijmm.2011.871. Epub 2011 Dec 29. Nanog And ??-Catenin: A New Convergence Point In Epsc Proliferation And Differentiation.||PubMed ID 22211239|
|Quan, J., Du, Q., Hou, Y., Wang, Z., & Zhang, J. (2017). Utilization of E-cadherin by monocytes from tumour cells plays key roles in the progression of bone invasion by oral squamous cell carcinoma. Oncology Reports, 38(2), 850-858. Advance online...||PubMed ID 28656299|
|Selective tropism of liver stem cells to hepatocellular carcinoma in vivo.||PubMed ID 29246405|
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