Anti-Galectin 3/LGALS3 Antibody Picoband™ (monoclonal, 12B12)

Boster Bio Anti-Galectin 3/LGALS3 Antibody Picoband™ (monoclonal, 12B12) catalog # M00621-3. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00621-3
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-Galectin 3/LGALS3 Antibody Picoband™ (monoclonal, 12B12)

See all Galectin-3 products

SKU/Catalog Number

M00621-3

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Galectin 3/LGALS3 Antibody Picoband™ (monoclonal, 12B12) catalog # M00621-3. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Galectin 3/LGALS3 Antibody Picoband™ (monoclonal, 12B12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00621-3)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

12B12

Isotype

IgG2a

Immunogen

E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequence identity with mouse and rat Galectin 3, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M00621-3 is reactive to LGALS3 in Human, Mouse, Rat

Applications

M00621-3 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For LGALS3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P17931

Gene ID

3958

Gene Name

LGALS3

Full Name

Galectin-3

Weight

26.152kDa

Alternative Names

AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3 LGALS3 CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2 galectin 3 galectin-3|35 kDa lectin|IgE-binding protein|MAC-2 antigen|advanced glycation end-product receptor 3|carbohydrate-binding protein 35|epididymis secretory sperm binding protein|galactose-specific lectin 3|laminin-binding protein|lectin L-29|lectin, galactoside-binding, soluble, 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LGALS3, check out the LGALS3 Infographic

LGALS3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LGALS3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Anti-Galectin 3/LGALS3 Antibody Picoband™ (monoclonal, 12B12)

Question

Would M00621-3 anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-05

Answer

You can see on the product datasheet, M00621-3 anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-05

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) M00621-3. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-10

Question

I was wanting to use to test anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) M00621-3 on human cervix carcinoma for research purposes, then I may be interested in using anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) M00621-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

K. Roberts

Verified customer

Asked: 2019-10-29

Answer

The products we sell, including anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) M00621-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-29

Question

Will anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) M00621-3 work on pig Flow Cytometry with skin?

L. Lewis

Verified customer

Asked: 2018-10-10

Answer

Our lab technicians have not validated anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) M00621-3 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) M00621-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig skin in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-10-10

Question

Is this M00621-3 anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) reactive to the isotypes of LGALS3?

A. Carter

Verified customer

Asked: 2016-12-28

Answer

The immunogen of M00621-3 anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) is E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequence identity with mouse and rat Galectin 3, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-12-28

Question

Is a blocking peptide available for product anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) (M00621-3)?

D. Zhang

Verified customer

Asked: 2016-12-22

Answer

We do provide the blocking peptide for product anti-Galectin 3/LGALS3 antibody (monoclonal, 12B12) (M00621-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-12-22

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M00621-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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