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- Table of Contents
BCAP31 antibody (A03767-4) performs well in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of A431 cells using anti-BAP31/BCAP31 antibody (A03767-4).
Overlay histogram showing A431 cells stained with A03767-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAP31/BCAP31 Antibody (A03767-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with A03767-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAP31/BCAP31 Antibody (A03767-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-BAP31/BCAP31 Antibody Picoband®
BCAP31 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
BCAP31 antibody was positively detected by WB in:
human 293T whole cell lysates, human A431 whole cell lysates, human Hela whole cell lysates, human placenta tissue lysates, rat liver tissue lysates, rat PC-12 whole cell lysates, mouse liver tissue lysates, mouse NIH/3T3 whole cell lysates
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
BCAP31 antibody was positively detected by IHC in:
human colon cancer tissue, human lung adenocarcinoma tissue, human ovarian cancer tissue, human placenta tissue, human thyroid cancer tissue, human tonsil tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
BCAP31 antibody was positively detected by ICC/IF in:
A549 cells
BCAP31 antibody was positively detected by IF in:
human colon cancer tissue, human prostate cancer tissue
BCAP31 antibody was positively detected by IP in:
A431 cells
BCAP31 antibody was positively detected by FC in:
A431 cells
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Immunoprecipitation
2-4 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-BAP31/BCAP31 Antibody Picoband®
View all BCAP31 Antibodies
View all BCAP31 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human BAP31/BCAP31 recombinant protein (Position: M1-D242). Human BAP31/BCAP31 shares 91.2% amino acid (aa) sequence identity with mouse BAP31/BCAP31.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
28 kDa
Calculated Molecular Weight
28.0 kDa
Background of BCAP31
B-cell receptor-associated protein 31 is a protein that in humans is encoded by the BCAP31 gene.
Cite This Product
Anti-BAP31/BCAP31 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03767-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rabbit
92%
High Homology
Rat
91%
High Homology
Mouse
90%
High Homology
Dog
90%
High Homology
Cow
87%
Moderate Homology
Sheep
87%
Moderate Homology
Zebrafish
60%
Low homology
Drosophila
38%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for BCAP31 antibody
Immunohistochemistry (IHC) protocol for BCAP31 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for BCAP31 antibody
Immunofluorescence (IF) protocol for BCAP31 antibody
Immunoprecipitation (IP) protocol for BCAP31 antibody
Flow Cytometry (FC) protocol for BCAP31 antibody
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Product Promise
We promise Anti-BAP31/BCAP31 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A03767-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026