Anti-BARD1 Antibody

BARD1 antibody

Boster Bio Anti-BARD1 Antibody (Catalog # A01646). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A01646
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-BARD1 Antibody

View all BARD1 Antibodies

SKU/Catalog Number

A01646

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-BARD1 Antibody (Catalog # A01646). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BARD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01646)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from N-terminal of human BARD1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01646 is reactive to BARD1 in Human

Applications

A01646 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

86.648kDa

Background of BARD1

Probable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.

Wu L.C., Nat. Genet. 14:430-440(1996).
Thai T.H., Hum. Mol. Genet. 7:195-202(1998).
Hillier L.W., Nature 434:724-731(2005).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For BARD1 (Source: Uniprot.org, NCBI)

Gene Name

BARD1

Full Name

BRCA1-associated RING domain protein 1

Weight

86.648kDa

Alternative Names

BARD-1; BRCA1 associated RING domain 1; BRCA1-associated RING domain gene 1; BRCA1-associated RING domain protein 1; EC 6.3.2.- BARD1 BRCA1 associated RING domain 1 BRCA1-associated RING domain protein 1|BRCA1-associated RING domain gene 1|RING-type E3 ubiquitin transferase BARD1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BARD1, check out the BARD1 Infographic

BARD1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BARD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-BARD1 Antibody

Question

Would A01646 anti-BARD1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

You can see on the product datasheet, A01646 anti-BARD1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-29

Question

Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-BARD1 antibody A01646. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-20

Question

We are currently using anti-BARD1 antibody A01646 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2017-09-25

Answer

The anti-BARD1 antibody (A01646) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-09-25

Order DetailsPrice
A01646-100uL

100uL

$360

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