Anti-Beclin-1 BECN1 Antibody

Immunofluorescence Validation of Beclin-1 in HeLa Cells
Immunofluorescent analysis of methanol-fixed HeLa cells labeling Beclin-1 with A00327-1 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

WB Validation in Human Cell Lines
Loading: 15 μg of lysate Antibodies: Beclin-1 A00327-1, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Mouse Tissues
Loading: 15 μg of lysate Antibodies: Beclin-1 A00327-1 , 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Tissues
Loading: 15 μg of lysate Antibodies: Beclin-1 A00327-1, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of Beclin-1 in Human kidney Tissue
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Beclin-1 antibody (A00327-1) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of Beclin-1 in Mouse Brain Tissue
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Beclin-1 antibody (A00327-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of Beclin-1 in Mouse Brain Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling Beclin-1 with A00327-1 at 20 ?g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).

Knockdown of Beclin-1 in Human T47D Cells (Thomas et al., 2011)
T47D cells were transfected with scrambled or Beclin-1 siRNA. The following day, these cells were treated with vehicle, C, 0.5 mM VPA, 10 lM 4OH-tamoxifen, T, or 0.5 mM VPA and 10 lM 4OH-tamoxifen, VT, for 48 h and viability was assayed by dye exclusion.

Immunohistochemistry Validation of Beclin-1 in Human HCC Cells (Qiu et al., 2014)
Beclin-1 exhibited cytoplasmic staining in adjacent non-tumor (ANT) tissues (A), bordeling site between HCC and ANT (B: left, ANT; right: HCC), and HCC (C), respectively. Beclin-1 expression was stronger in ANT than in HCC. Magnification × 400.

Immunohistochemistry Validation of Beclin-1 in Rat Brain Tissue
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Beclin-1 antibody (A00327-1) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of Beclin-1 in Rat Liver Tissue
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Beclin-1 antibody (A00327-1) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescent Validation of Beclin-1 in Human Cortical Neurons and Rat Brain (Wang et al., 2017)
Cell specificity of Beclin 1 expression. Cortical neurons and glia co-cultures (upper panels) and cortex slices (lower panels) were immunostained with anti-Beclin 1 (red) and NeuN (neuronal biomarker, green) or GFAP (astrocyte biomarker, green). Neurons and astrocyte in cultured cells were indicated by arrows and arrowhead, respectively. Areas in white boxes were enlarged.

Regulated Expression of Beclin-1 in Human T47D Cells (Thomas et al., 2011)
Treatment with tamoxifen and an HDAC inhibitor alters the balance of autophagy and apoptosis inhibitors and drivers. T47D cells were treated with vehicle, C, 0.5 mM VPA, V, 10 lM 4OHtamoxifen, T, or 0.5 mM VPA and 10 lM 4OH-tamoxifen, VT, for 48 h and Beclin-1 protein and RNA