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Product Info Summary
| SKU: | PA1847 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Beclin 1/BECN1 Antibody Picoband®
SKU/Catalog Number
PA1847
BA3123-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Beclin 1/BECN1 Antibody catalog # PA1847. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Beclin 1/BECN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1847)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Beclin 1, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1847 is reactive to BECN1 in Human, Mouse, Rat
Observed Molecular Weight
46 kDa
Calculated molecular weight
51.9 kDa
Background of BECN1
Bellini-1 (beclin 1, autophagy related) is a protein that in humans is encoded by the BECN1 gene, also known as BECN1, ATG6, VPS30, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, autophagy-related gene (Atg) 6. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. Human beclin encodes a novel 450-amino acid protein containing a coiled-coil region, within which it has limited homology to myosin-like proteins. Northern blot analysis of mouse and adult human tissues revealed widespread beclin expression. A 2.2-kb transcript was present at highest levels in human skeletal muscle and at detectable levels in all tissues examined. In some tissues, additional 1.7- and 1.4-kb transcripts were observed, suggesting the presence of alternatively spliced transcripts. Immunoperoxidase staining of human hippocampus and frontal cortex sections revealed beclin immunoreactivity in many neurons throughout these regions, as well as in some glial cells. Beclin protects against infection by a neurovirulent strain of Sindbis virus that is known to overcome the protective effects of Bcl2. Liang et al. showed that beclin reduced Sindbis virus replication in mouse brain and reduced Sindbis virus-induced cell death in mouse brain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1847 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: HELA Cell, SW620 Cell, PANC Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-Beclin 1 antibody, PA1847, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: PANC Cell Lysate
Specific Publications For Anti-Beclin 1/BECN1 Antibody Picoband® (PA1847)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Beclin 1/BECN1 Antibody Picoband®
Question
Will PA1847 anti-Beclin 1/BECN1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Brown
Verified customer
Asked: 2020-04-07
Answer
As indicated on the product datasheet, PA1847 anti-Beclin 1/BECN1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-07
Question
We are currently using anti-Beclin 1/BECN1 antibody PA1847 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
The anti-Beclin 1/BECN1 antibody (PA1847) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-19
Question
Is this PA1847 anti-Beclin 1/BECN1 antibody reactive to the isotypes of BECN1?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
The immunogen of PA1847 anti-Beclin 1/BECN1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human Beclin 1(222-237aa LDQEEAQYQREYSEFK), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-29
Question
Our lab want to know about to test anti-Beclin 1/BECN1 antibody PA1847 on rat liver for research purposes, then I may be interested in using anti-Beclin 1/BECN1 antibody PA1847 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
The products we sell, including anti-Beclin 1/BECN1 antibody PA1847, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-14
Question
you antibody using your anti-Beclin 1/BECN1 antibody for cellular response to amino acid starvation studies. Has this antibody been tested with western blotting on sw620 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
I appreciate your inquiry. This PA1847 anti-Beclin 1/BECN1 antibody is validated on hela cell lysate, sw620 cell lysate, panc cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-24
Question
See attached the WB image, lot number and protocol we used for liver using anti-Beclin 1/BECN1 antibody PA1847. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-27
Question
Is a blocking peptide available for product anti-Beclin 1/BECN1 antibody (PA1847)?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
We do provide the blocking peptide for product anti-Beclin 1/BECN1 antibody (PA1847). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-09
Question
My boss were well pleased with the WB result of your anti-Beclin 1/BECN1 antibody. However we have seen positive staining in frontal cortex cytoplasm using this antibody. Is that expected? Could you tell me where is BECN1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
From literature, frontal cortex does express BECN1. Generally BECN1 expresses in cytoplasm. Regarding which tissues have BECN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9765397, 10395800
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 14702039
Mammary gland, Pubmed ID: 7490091
Boster Scientific Support
Answered: 2019-08-07
Question
My question regarding product PA1847, anti-Beclin 1/BECN1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Williams
Verified customer
Asked: 2019-08-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1847 anti-Beclin 1/BECN1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-07
Question
Would anti-Beclin 1/BECN1 antibody PA1847 work on primate WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
Our lab technicians have not tested anti-Beclin 1/BECN1 antibody PA1847 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Beclin 1/BECN1 antibody PA1847 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-10
Question
I was wanting to use your anti-Beclin 1/BECN1 antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
You can see on the product datasheet, PA1847 anti-Beclin 1/BECN1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Beclin 1/BECN1 antibody PA1847. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-10-02
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-02
Question
Would anti-Beclin 1/BECN1 antibody PA1847 work for WB with liver?
C. Anderson
Verified customer
Asked: 2016-12-28
Answer
According to the expression profile of liver, BECN1 is highly expressed in liver. So, it is likely that anti-Beclin 1/BECN1 antibody PA1847 will work for WB with liver.
Boster Scientific Support
Answered: 2016-12-28
Question
We have observed staining in human liver. What should we do? Is anti-Beclin 1/BECN1 antibody supposed to stain liver positively?
A. Taylor
Verified customer
Asked: 2016-10-12
Answer
From what I have seen in literature liver does express BECN1. From what I have seen in Uniprot.org, BECN1 is expressed in frontal cortex, brain, liver, cervix, mammary gland, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have BECN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9765397, 10395800
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 14702039
Mammary gland, Pubmed ID: 7490091
Boster Scientific Support
Answered: 2016-10-12
Question
I see that the anti-Beclin 1/BECN1 antibody PA1847 works with WB, what is the protocol used to produce the result images on the product page?
J. Krishna
Verified customer
Asked: 2016-03-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-03-02
Question
Do you have a BSA free version of anti-Beclin 1/BECN1 antibody PA1847 available?
E. Krishna
Verified customer
Asked: 2013-07-09
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Beclin 1/BECN1 antibody PA1847 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-07-09
