Anti-Transferrin/TF Antibody

Boster Bio Anti-Transferrin/TF Antibody catalog # RP1022. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. Cited in 7 publication(s).

Product Info Summary

SKU: RP1022
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-Transferrin/TF Antibody

See all Transferrin products

SKU/Catalog Number

RP1022

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Transferrin/TF Antibody catalog # RP1022. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Transferrin/TF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1022)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

Oryza sativa-derived human Transferrin recombinant protein (Position: V20-P698).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1022 is reactive to TF in Human

Applications

RP1022 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For TF (Source: Uniprot.Org, NCBI)

Uniprot ID

P02787

Gene ID

7018

Gene Name

TF

Full Name

Serotransferrin

Weight

77.064kDa

Superfamily

transferrin family

Alternative Names

Beta-1 metal-binding globulin; DKFZp781D0156; EC 3.4.21; PRO1557; PRO2086; Serotransferrin; Siderophilin; TF; Transferrin TF HEL-S-71p, PRO1557, PRO2086QTL1, TF transferrin serotransferrin|beta-1 metal-binding globulin|epididymis secretory sperm binding protein Li 71p|siderophilin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TF, check out the TF Infographic

TF infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1022 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Xiang Y,Zheng Y,Liu S,Liu G,Li Z,Dong W.Comparison of the sensitivity of Western blotting between PVDF and NC membranes.Sci Rep.2021 Jun 8;11(1):12022.doi:10.1038/s41598-021-91521-8.PMID:34103620;PMCID:PMC8187435.
Species: Human
RP1022 usage in article: APP:WB, SAMPLE:TPC-1 CELL, DILUTION:1:2000

MCPIP is induced by cholesterol and participated in cholesterol-caused DNA damage in HUVEC

Chen L, Luo G, Tan Y, Wei J, Wu C, Zheng L, Zhang X, Xu N. Acta Histochem. 2010 May;112(3):233-9. Doi: 10.1016/J.Acthis.2008.11.024. Epub 2009 May 9. Immunolocalisation Of Tissue Factor In Esophageal Cancer Is Correlated With Intratumoral Angiogen...

Chen K, Li Z, Jiang P, Zhang X, Zhang Y, Jiang Y, He Y, Li X. Oncol Rep. 2014 Aug;32(2):755-63. Doi: 10.3892/Or.2014.3245. Epub 2014 Jun 11. Co-Expression Of Cd133, Cd44V6 And Human Tissue Factor Is Associated With Metastasis And Poor Prognosis In...

Liang W, Zhang W, Zhao S, Li Q, Yang Y, Liang H, Ceng R. Mol Med Rep. 2015 Feb;11(2):903-9. Doi: 10.3892/Mmr.2014.2822. Epub 2014 Oct 30. A Study Of The Ultrasound-Targeted Microbubble Destruction Based Triplex-Forming Oligodexinucleotide Delivery...

Zhu W, Lv Q, Chen H, Wang Z, Zhong Q. J Huazhong Univ Sci Technolog Med Sci. 2011 Aug;31(4):441-5. Doi: 10.1007/S11596-011-0470-8. Epub 2011 Aug 7. Protective Effect And Mechanism Of Sodium Tanshinone Ii A Sulfonate On Microcirculatory Disturbance...

Wang G, Hu W, Tang Q, Wang L, Sun Xg, Chen Y, Yin Y, Xue F, Sun Z. Mol Neurobiol. 2015 Jun 23. [Epub Ahead Of Print] Effect Comparison Of Both Iron Chelators On Outcomes, Iron Deposit, And Iron Transporters After Intracerebral Hemorrhage In Rats.

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16 Customer Q&As for Anti-Transferrin/TF Antibody

Question

I see that the anti-Transferrin/TF antibody RP1022 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-06

Question

Is this RP1022 anti-Transferrin/TF antibody reactive to the isotypes of TF?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

The immunogen of RP1022 anti-Transferrin/TF antibody is Oryza sativa-derived human Transferrin recombinant protein(Position: V20-P698). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-18

Question

We have tried in the past anti-Transferrin/TF antibody for ELISA on liver in a previous experiment. I am using human, and We are going to use the antibody for IHC next. I am looking for examining liver as well as inferior vagus x ganglion in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

I looked at the website and datasheets of our anti-Transferrin/TF antibody and it appears that RP1022 has been validated on human in both ELISA and IHC. Thus RP1022 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-31

Question

Will RP1022 anti-Transferrin/TF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

It shows on the product datasheet, RP1022 anti-Transferrin/TF antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-29

Question

Would anti-Transferrin/TF antibody RP1022 work for IHC with bile?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

According to the expression profile of bile, TF is highly expressed in bile. So, it is likely that anti-Transferrin/TF antibody RP1022 will work for IHC with bile.

Boster Scientific Support

Answered: 2020-01-01

Question

Will anti-Transferrin/TF antibody RP1022 work on zebrafish ELISA with saliva?

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

Our lab technicians have not validated anti-Transferrin/TF antibody RP1022 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Transferrin/TF antibody RP1022 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish saliva in ELISA, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-14

Question

I have a question about product RP1022, anti-Transferrin/TF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1022 anti-Transferrin/TF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-06

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bile using anti-Transferrin/TF antibody RP1022. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-24

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-24

Question

Is a blocking peptide available for product anti-Transferrin/TF antibody (RP1022)?

Verified Customer

Verified customer

Asked: 2018-02-21

Answer

We do provide the blocking peptide for product anti-Transferrin/TF antibody (RP1022). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-02-21

Question

We have been able to see staining in human liver. What should we do? Is anti-Transferrin/TF antibody supposed to stain liver positively?

C. Johnson

Verified customer

Asked: 2018-01-29

Answer

From literature liver does express TF. From Uniprot.org, TF is expressed in inferior vagus x ganglion, liver, brain, fetal liver, brain, cajal-retzius cell fetal brain cortex, heart, bile, plasma, saliva, cerebrospinal fluid, among other tissues. Regarding which tissues have TF expression, here are a few articles citing expression in various tissues:
Bile, Pubmed ID: 15084671
Brain, Pubmed ID: 9272172, 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Heart, Pubmed ID: 7498159
Liver, Pubmed ID: 1809186, 19159218, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Saliva, Pubmed ID: 16740002

Boster Scientific Support

Answered: 2018-01-29

Question

See attached the WB image, lot number and protocol we used for bile using anti-Transferrin/TF antibody RP1022. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-01-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-11

Question

We are currently using anti-Transferrin/TF antibody RP1022 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2017-12-26

Answer

The anti-Transferrin/TF antibody (RP1022) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-12-26

Question

Do you have a BSA free version of anti-Transferrin/TF antibody RP1022 available?

Verified Customer

Verified customer

Asked: 2017-12-07

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Transferrin/TF antibody RP1022 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-12-07

Question

I am looking for to test anti-Transferrin/TF antibody RP1022 on human bile for research purposes, then I may be interested in using anti-Transferrin/TF antibody RP1022 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Johnson

Verified customer

Asked: 2016-10-06

Answer

The products we sell, including anti-Transferrin/TF antibody RP1022, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-10-06

Question

I was wanting to use your anti-Transferrin/TF antibody for IHC for human bile on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bile identification?

J. Carter

Verified customer

Asked: 2016-01-18

Answer

As indicated on the product datasheet, RP1022 anti-Transferrin/TF antibody has been tested for ELISA, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bile in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-01-18

Question

My team were well pleased with the WB result of your anti-Transferrin/TF antibody. However we have been able to see positive staining in liver secreted. using this antibody. Is that expected? Could you tell me where is TF supposed to be expressed?

M. Collins

Verified customer

Asked: 2013-12-12

Answer

From literature, liver does express TF. Generally TF expresses in secreted. Regarding which tissues have TF expression, here are a few articles citing expression in various tissues:
Bile, Pubmed ID: 15084671
Brain, Pubmed ID: 9272172, 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Heart, Pubmed ID: 7498159
Liver, Pubmed ID: 1809186, 19159218, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Saliva, Pubmed ID: 16740002

Boster Scientific Support

Answered: 2013-12-12

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