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Product Info Summary
|Application:||ELISA, IHC, WB|
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Boster Bio Anti-Transferrin/TF Antibody catalog # RP1022. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transferrin/TF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1022)
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Oryza sativa-derived human Transferrin recombinant protein (Position: V20-P698).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
RP1022 is reactive to TF in Human
RP1022 is guaranteed for ELISA, IHC, WB Boster Guarantee
Background of Transferrin
Transferrins are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. In humans, it is encoded by the TF gene. In humans, transferrin consists of a polypeptide chain containing 679 amino acids. The protein is composed of alpha helices and beta sheets to form two domains. The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). Transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe (III) binding sites. The affinity of transferrin for Fe (III) is extremely high (1023 M−1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure. Western blot analysis of Transferrin using anti-Transferrin antibody (RP1022).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane : Recombinant Human Transferrin Protein 0.5ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Transferrin antigen affinity purified polyclonal antibody (Catalog # RP1022) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Transferrin at approximately 76KD. The expected band size for Transferrin is at 76KD.
Gene/Protein Information For TF (Source: Uniprot.org, NCBI)
Beta-1 metal-binding globulin; DKFZp781D0156; EC 3.4.21; PRO1557; PRO2086; Serotransferrin; Siderophilin; TF; Transferrin TF HEL-S-71p, PRO1557, PRO2086QTL1, TF transferrin serotransferrin|beta-1 metal-binding globulin|epididymis secretory sperm binding protein Li 71p|siderophilin*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on TF, check out the TF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
RP1022 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xiang Y,Zheng Y,Liu S,Liu G,Li Z,Dong W.Comparison of the sensitivity of Western blotting between PVDF and NC membranes.Sci Rep.2021 Jun 8;11(1):12022.doi:10.1038/s41598-021-91521-8.PMID:34103620;PMCID:PMC8187435.
RP1022 usage in article: APP:WB, SAMPLE:TPC-1 CELL, DILUTION:1:2000
MCPIP is induced by cholesterol and participated in cholesterol-caused DNA damage in HUVEC
Chen L, Luo G, Tan Y, Wei J, Wu C, Zheng L, Zhang X, Xu N. Acta Histochem. 2010 May;112(3):233-9. Doi: 10.1016/J.Acthis.2008.11.024. Epub 2009 May 9. Immunolocalisation Of Tissue Factor In Esophageal Cancer Is Correlated With Intratumoral Angiogen...
Chen K, Li Z, Jiang P, Zhang X, Zhang Y, Jiang Y, He Y, Li X. Oncol Rep. 2014 Aug;32(2):755-63. Doi: 10.3892/Or.2014.3245. Epub 2014 Jun 11. Co-Expression Of Cd133, Cd44V6 And Human Tissue Factor Is Associated With Metastasis And Poor Prognosis In...
Liang W, Zhang W, Zhao S, Li Q, Yang Y, Liang H, Ceng R. Mol Med Rep. 2015 Feb;11(2):903-9. Doi: 10.3892/Mmr.2014.2822. Epub 2014 Oct 30. A Study Of The Ultrasound-Targeted Microbubble Destruction Based Triplex-Forming Oligodexinucleotide Delivery...
Zhu W, Lv Q, Chen H, Wang Z, Zhong Q. J Huazhong Univ Sci Technolog Med Sci. 2011 Aug;31(4):441-5. Doi: 10.1007/S11596-011-0470-8. Epub 2011 Aug 7. Protective Effect And Mechanism Of Sodium Tanshinone Ii A Sulfonate On Microcirculatory Disturbance...
Wang G, Hu W, Tang Q, Wang L, Sun Xg, Chen Y, Yin Y, Xue F, Sun Z. Mol Neurobiol. 2015 Jun 23. [Epub Ahead Of Print] Effect Comparison Of Both Iron Chelators On Outcomes, Iron Deposit, And Iron Transporters After Intracerebral Hemorrhage In Rats.
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