Anti-Beta TrCP2 FBXW11 Antibody

Product Name

Anti-Beta TrCP2 FBXW11 Antibody

View all FBXW11 Antibodies

SKU/Catalog Number

A05523

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Beta TrCP2 FBXW11 Antibody (Catalog # A05523). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Beta TrCP2 FBXW11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05523)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human ßTrCP2 protein.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05523 is reactive to FBXW11 in Human, Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

62.1 kDa

Background of FBXW11

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05523 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 – 1:100,000
IF Microscopy: User optimized
WB: 1:200 to 1:1,000
This affinity purified antibody has been tested for use in ELISA and western blotting.

Validation Images & Assay Conditions

See full target information FBXW11

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