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TUBB antibody (A01857-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Chicken, Human, Monkey, Mouse, Rat, Zebrafish.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 37 Publications

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a01857 1 tubb primary antibodies fcm testing 5_1

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Flow Cytometry analysis of SiHa cells using anti-Beta Tubulin antibody (A01857-1).
Overlay histogram showing SiHa cells stained with A01857-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Beta Tubulin Antibody (A01857-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-Beta Tubulin/TUBB Antibody Picoband®

Name: Anti-Beta Tubulin/TUBB Antibody Picoband®
Brand: Boster Bio
SKU: A01857-1
Price: 370 USD
Availability: InStock
Rating: 100 (2 reviews)

TUBB antibody

37 Publications|

2 Reviews

|Submit a Review

Reactive Species	Host	Application
Chicken, Human, Monkey, Mouse, Rat, Zebrafish	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

TUBB antibody was positively detected by WB in:

human SiHa whole cell, human 293T whole cell, human HepG2 whole cell, monkey COS-7 whole cell, chicken heart tissue, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell, human HeLa whole cell, human MCF-7 whole cell, zebrafish embryo

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

TUBB antibody was positively detected by IHC in:

human liver cancer tissue, human testicular germ cell tumor tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

TUBB antibody was positively detected by ICC/IF in:

U20S cell

TUBB antibody was positively detected by FC in:

SiHa cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Monkey

Tested

Chicken

Tested

Rat

Tested

Mouse

Tested

Zebrafish

Tested

Rabbit

Predicted

Sheep

Predicted

Dog

Predicted

Fruit fly

Predicted

Product Information

Product Name

Anti-Beta Tubulin/TUBB Antibody Picoband®
View all TUBB Antibodies

Form

Lyophilized

Uniprot

P07437

Gene ID

203068

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Beta Tubulin, identical to the related mouse and rat sequences.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

50 kDa

Calculated Molecular Weight

49.7 kDa

Background of TUBB

Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Cite This Product

Anti-Beta Tubulin/TUBB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01857-1)

Protocols

Western Blot protocol for TUBB antibody

Review Protocol

Immunohistochemistry (IHC) protocol for TUBB antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TUBB antibody

Review Protocol

Flow Cytometry (FC) protocol for TUBB antibody

Review Protocol

Publications

16α-Hydroxydehydrotrametenolic acid attenuates MDA-MB-231 triple-negative breast cancer xenograft growth and modulates integrin β1/c-Met signaling and glycolytic metabolism

Published: 2026-08-19

Journal: PHYTOMEDICINE

Application: WB

Impact Factor: 11.30

Species: Human

PMID: 42641546

SKU	A01857-1
Application	Immunocytochemistry / Immunofluorescence
Sample	Human HepG2 cells
Sample Processing Description	Human HepG2 cells on coverslips were treated with pepsin at room temperature for 10 minutes, followed by antigen retrieval using a composite digestion solution for 10 minutes.
Other Reagents	Mixed Enzyme Digestion Solution; DAPI Staining Solution
Primary Antibody	Beta Tubulin/TUBB Antibody Picoband®
Primary Incubation	1:100, overnight at 4 ℃
Secondary Antibody	Fluoro 488-Goat Anti-Rabbit (H+L) (Boster BA1127)
Secondary Incubation	Incubate at 37°C for 30 minutes
Detection	Imaging system: Leica DMi3000 Inverted Fluorescence Microscope
Results Summary	This image shows the results of co-staining for SIRT1 mRNA (via FISH) and beta-tubulin (via IF) at 400x magnification; green fluorescence indicates positive beta-tubulin staining, while red indicates the mRNA FISH signal. Beta-tubulin is a major component of microtubules, one of the three primary fibrous systems constituting the cytoskeleton. Microtubules are essential for maintaining cell morphology and facilitating processes such as intracellular transport, cell division, and cell motility; the co-staining results demonstrate clear visualization and precise localization within the cytoskeleton.

SKU	A01857-1
Application	Western Blot
Sample	human OCI-LY1 cells
Sample Processing Description	Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of each sample were loaded per well.
Other Reagents	5% Non-fat milk
Primary Antibody	Beta Tubulin/TUBB Antibody
Primary Incubation	1:3000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation	1 hour in room temperature
Detection	Substrate: ECL luminescent reagent, Imaging system:ChemiDoc MP
Results Summary	The antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has clear and well-defined edges.

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Anti-Beta Tubulin/TUBB Antibody Picoband®

TUBB antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

16α-Hydroxydehydrotrametenolic acid attenuates MDA-MB-231 triple-negative breast cancer xenograft growth and modulates integrin β1/c-Met signaling and glycolytic metabolism

The SIRT1-ΔExon8 transcript is differentially expressed across brain regions and during neural stem cell differentiation in rats

Delphinidin, a dietary anthocyanidin, modulates PD-L1 and STING/TBK1 signaling and reprograms the immune microenvironment in triple-negative breast cancer

BNIP3/BNIP3L-Dependent Mitophagy Protects Against Hippocampal Neuronal Damage and Apoptosis in a Model of Vascular Dementia

Bupleurum chinense Ameliorates Metabolic-Associated Fatty Liver Disease by Modulating Sirtuin 6

Functionalized copper-depleting nanosponges for breast cancer dual starvation favoring dependent energy metabolism and angiogenesis

Effects and Mechanisms of NMDAR-EphB2 on Synapses in Mice by Active Immunization with a GluN1 Peptide

The Electric Field Guided HaCaT Cell Migration Through the EGFR/p38 MAPK/Akt Pathway

CCR7/DUSP1 signaling Axis mediates iCAF to regulates head and neck squamous cell carcinoma growth

The Study of SRSF1 Regulates Abnormal Alternative Splicing of BCL2L11 and the Role in Refractory Acute Myeloid Leukemia

Enhanced therapeutic potential of Flotillins-modified MenSCs by improve the survival, proliferation and migration

Limonin alleviates high-fat diet-induced dyslipidemia by regulating intestinal barrier via microbiota-related ILC3-IL22-IL22R pathway

Analysis of the potential biological value of pyruvate dehydrogenase E1 subunit β in human cancer

Recombinant Slit2 attenuates tracheal fibroblast activation in benign central airway obstruction by inhibiting the TGF-β1/Smad3 signaling pathway

CCDC167 promotes hepatocellular carcinoma cell proliferation and is associated with the immune microenvironment of hepatocellular carcinoma

Cyclovirobuxine D pretreatment ameliorates septic heart injury through mitigation of ferroptosis

Organotin benzohydroxamate derivatives (OTBH) target colchicine-binding site exerting potent antitumor activity both in vitro and vivo revealed by quantitative proteomic analysis

Andrographolide induced ferroptosis in multiple myeloma cells by regulating the P38/Nrf2/HO-1 pathway

Biological effects of exposure to 2650 MHz electromagnetic radiation on the behavior, learning, and memory of mice

Water extract of cacumen platycladi promotes hair growth through the Akt/GSK3β/β-catenin signaling pathway

Comparison of protective effects of hesperetin and pectolinarigenin on high-fat diet-induced hyperlipidemia and hepatic steatosis in Golden Syrian hamsters

ELOVL2 restrains cell proliferation, migration, and invasion of prostate cancer via regulation of the tumor suppressor INPP4B

Palmatine attenuates LPS-induced inflammatory response in mouse mammary epithelial cells through inhibiting ERK1/2, P38 and Akt/NF-кB signalling pathways

Dehydroandrographolide inhibits mastitis by activating autophagy without affecting intestinal flora

Neuroprotective efficacy of different levels of high-frequency repetitive transcranial magnetic stimulation in mice with CUMS-induced depression: Involvement of the p11/BDNF/Homer1a signaling pathway

Stathmin Regulates Spatiotemporal Variation in the Memory Loop in Single-Prolonged Stress Rats

IL-27 is elevated in sepsis with acute hepatic injury and promotes hepatic damage and inflammation in the CLP model

A Novel lnc-RNA, Named lnc-ORA, Is Identified by RNA-Seq Analysis, and Its Knockdown Inhibits Adipogenesis by Regulating the PI3K/AKT/mTOR Signaling Pathway

Dehydroepiandrosterone Prevents H2O2-Induced BRL-3A Cell Oxidative Damage through Activation of PI3K/Akt Pathways rather than MAPK Pathways

Brain Infection by Hepatitis E Virus Probably via Damage of the Blood-Brain Barrier Due to Alterations of Tight Junction Proteins

Licochalcone A Protects the Blood–Milk Barrier Integrity and Relieves the Inflammatory Response in LPS-Induced Mastitis

Vanillin protects the blood–milk barrier and inhibits the inflammatory response in LPS-induced mastitis in mice

Retinoic Acid-Induced Protein 14 (RAI14) Promotes mTOR-Mediated Inflammation Under Inflammatory Stress and Chemical Hypoxia in a U87 Glioblastoma Cell Line

Peiminine Protects against Lipopolysaccharide-Induced Mastitis by Inhibiting the AKT/NF-κB, ERK1/2 and p38 Signaling Pathways

M1-like tumor-associated macrophages activated by exosome-transferred THBS1 promote malignant migration in oral squamous cell carcinoma

TLR4 deficiency abrogated widespread tactile allodynia, but not widespread thermal hyperalgesia and trigeminal neuropathic pain after partial infraorbital nerve transection

Stemness distinctions between the ectomesenchymal stem cells from neonatal and adult mice

Review

Have you used Anti-Beta Tubulin/TUBB Antibody Picoband®?

2 Reviews For Anti-Beta Tubulin/TUBB Antibody Picoband®

Anti-TUBB Antibody (A01857-1) produced distinct green fluorescent signals with low background in 400× co-staining (FISH + IF) of HepG2 cells, showing precise localization of beta-tubulin within the cytoskeleton.

In WB using β-tubulin antibody (Cat# A01857-1), a clear, specific band was observed in OCI-LY8 cells with clean background, showing stable expression after SRPK1 knockdown.

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