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- Table of Contents
TUBB antibody (M05613) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Immunofluorescent analysis of NIH/3T3 cells, using beta Tubulin Antibody .
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Anti-beta Tubulin TUBB Rabbit Monoclonal Antibody
TUBB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
TUBB antibody was positively detected by WB in:
human Hela whole cell, monkey COS-7 whole cell, rat PC-12 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TUBB antibody was positively detected by IHC in:
Human glioma, Rat brain, Mouse kidney, Human uterus cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TUBB antibody was positively detected by ICC/IF in:
NIH/3T3 cell
Recommend Dilution
Application
Dilution
Species
WB 1:3000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
FC
Human
Tested
Tested
Recommend
Recommend
Monkey
Tested
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-beta Tubulin TUBB Rabbit Monoclonal Antibody
View all TUBB Antibodies
View all TUBB Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BI-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta Tubulin
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
49.7 kDa
Cite This Product
Anti-beta Tubulin TUBB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05613)
Protocols
Western Blot protocol for TUBB antibody
Immunohistochemistry (IHC) protocol for TUBB antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TUBB antibody
Publications
Application:
Species:
Quercetin in Shengxian Decoction exhibits anti-ferroptosis protective roles in a myocardial infarction model via targeting DPP4/ HMOX1, based on network pharmacology and molecular docking
Published: 2025-04-29
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.80
Species: Rat
PMID: 40365322
Electro-Driven Surface-Plus PtIr Nanocatalyst Degradation and Ion Channel Activation for Enhanced Tumor Dynamic Metal-ion Therapy with Minimal Toxicity
Published: 2025-02-09
Journal: ADVANCED FUNCTIONAL MATERIALS
Application: WB
Impact Factor: 19.00
Species: Mouse,Human
Alginate oligosaccharides relieve estrogen-deprived osteosarcopenia by affecting intestinal Th17 differentiation and systemic inflammation through the manipulation of bile acid metabolism
Published: 2025-01-07
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 8.50
Species: Mouse
PMID: 39788237
Engineered Magneto-Piezoelectric Nanoparticles-Enhanced Scaffolds Disrupt Biofilms and Activate Oxidative Phosphorylation in Icam1+ Macrophages for Infectious Bone Defect Regeneration
Published: 2024-12-17
Journal: ACS Nano
Application: WB
Impact Factor: 15.80
Species: Rat
PMID: 39690461
CCR7/DUSP1 signaling Axis mediates iCAF to regulates head and neck squamous cell carcinoma growth
Published: 2024-07-25
Journal: CELLULAR SIGNALLING
Application: WB
Impact Factor: 4.40
Species: Mouse
PMID: 39067836
Ferroptosis of brain microvascular endothelial cells contributes to hypoxia-induced blood–brain barrier injury
Published: 2023-04-12
Journal: FASEB JOURNAL
Application: WB
Impact Factor: 4.80
Species: Fish
PMID: 37043308
The upregulation of keratocan promotes the progression of human pancreatic cancer
Published: 2023-03-03
Journal: Molecular & Cellular Toxicology
Application: WB
Impact Factor: 1.70
Species: Human
TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis
Published: 2022-01-14
Journal: Biology-Basel
Application: WB
Impact Factor: 5.17
Species: Human
PMID: 35053139
Review
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Product Promise
We promise Anti-beta Tubulin TUBB Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M05613
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026