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Product Info Summary
| SKU: | PA1807 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Polycomb complex protein BMI-1 Bmi1 Antibody Picoband®
SKU/Catalog Number
PA1807
BA3771 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Polycomb complex protein BMI-1 Bmi1 Antibody catalog # PA1807. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Polycomb complex protein BMI-1 Bmi1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1807)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Bmi1, different from the related mouse sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1807 is reactive to BMI1 in Human
Observed Molecular Weight
40 kDa
Calculated molecular weight
36.9 kDa
Background of BMI1
BMI1 (BMI1 polycomb ring finger oncogene),also known as RNF51, is a protein which in humans is encoded by the BMI1 gene. The Bmi1 gene is highly conserved in evolution as indicated by zoo blot hybridization with Bmi1 probes corresponding to the protein-encoding domain. By fluorescence in situ hybridization, the human BMI1 gene is assigned to chromosome 10p13. BMI1 has a key role in regulating the proliferative activity of normal stem and progenitor cells. Most importantly, they provided evidence that the proliferative potential of leukemic stem and progenitor cells lacking BMI1 is compromised because they eventually undergo proliferation arrest and show signs of differentiation and apoptosis, leading to transplant failure of the leukemia. Complementation studies showed that BMI1 completely rescues these proliferative defects. Deletion analysis showed that the RING finger and helix-turn-helix domains of BMI1 were required for life span extension and repression of the tumor suppressor p16 (INK4). BMI1 selectively extended the life span of these cultures. Confocal microscopy showed that BMI1 transiently colocalized with centromeres during interphase in HeLa cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1807 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human 293T whole cell, human A431 whole cell, human U251 whole cell, human K562 whole cell
Validation Images & Assay Conditions
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Western blot analysis of BMI1 using anti-BMI1 antibody (PA1807).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMI1 antigen affinity purified polyclonal antibody (PA1807) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BMI1 at approximately 40 kDa. The expected band size for BMI1 is at 37 kDa.
Specific Publications For Anti-Polycomb complex protein BMI-1 Bmi1 Antibody Picoband® (PA1807)
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7 Customer Q&As for Anti-Polycomb complex protein BMI-1 Bmi1 Antibody Picoband®
Question
Here is the WB image, lot number and protocol we used for muscle using anti-Bmi1 antibody PA1807. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-01
Question
I see that the anti-Bmi1 antibody PA1807 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-03-26
Question
Would PA1807 anti-Bmi1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
It shows on the product datasheet, PA1807 anti-Bmi1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-04
Question
Is there a BSA free version of anti-Bmi1 antibody PA1807 available?
V. Wu
Verified customer
Asked: 2019-04-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bmi1 antibody PA1807 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-02
Question
Will anti-Bmi1 antibody PA1807 work for WB with muscle?
B. Parker
Verified customer
Asked: 2018-08-24
Answer
According to the expression profile of muscle, BMI1 is highly expressed in muscle. So, it is likely that anti-Bmi1 antibody PA1807 will work for WB with muscle.
Boster Scientific Support
Answered: 2018-08-24
Question
Is this PA1807 anti-Bmi1 antibody reactive to the isotypes of BMI1?
G. Zhao
Verified customer
Asked: 2018-02-26
Answer
The immunogen of PA1807 anti-Bmi1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human Bmi1(136-152aa EFFDQNRLDRKVNKDKE), different from the related mouse sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-26
Question
I have a question about product PA1807, anti-Bmi1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Anderson
Verified customer
Asked: 2016-02-08
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1807 anti-Bmi1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-02-08
