Anti-Polycomb complex protein BMI-1 Bmi1 Antibody

BMI-1 antibody

Boster Bio Anti-Polycomb complex protein BMI-1 Bmi1 Antibody catalog # PA1807. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA1807
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Polycomb complex protein BMI-1 Bmi1 Antibody

View all BMI-1 Antibodies

SKU/Catalog Number

PA1807

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Polycomb complex protein BMI-1 Bmi1 Antibody catalog # PA1807. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Polycomb complex protein BMI-1 Bmi1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1807)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Bmi1, different from the related mouse sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1807 is reactive to BMI1 in Human

Applications

PA1807 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

36.949kDa

Background of BMI-1

BMI1 (BMI1 polycomb ring finger oncogene),also known as RNF51, is a protein which in humans is encoded by the BMI1 gene. The Bmi1 gene is highly conserved in evolution as indicated by zoo blot hybridization with Bmi1 probes corresponding to the protein-encoding domain. By fluorescence in situ hybridization, the human BMI1 gene is assigned to chromosome 10p13. BMI1 has a key role in regulating the proliferative activity of normal stem and progenitor cells. Most importantly, they provided evidence that the proliferative potential of leukemic stem and progenitor cells lacking BMI1 is compromised because they eventually undergo proliferation arrest and show signs of differentiation and apoptosis, leading to transplant failure of the leukemia. Complementation studies showed that BMI1 completely rescues these proliferative defects. Deletion analysis showed that the RING finger and helix-turn-helix domains of BMI1 were required for life span extension and repression of the tumor suppressor p16 (INK4). BMI1 selectively extended the life span of these cultures. Confocal microscopy showed that BMI1 transiently colocalized with centromeres during interphase in HeLa cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For BMI1 (Source: Uniprot.org, NCBI)

Gene Name

BMI1

Full Name

Polycomb complex protein BMI-1

Weight

36.949kDa

Alternative Names

B lymphoma Mo-MLV insertion region 1 homolog (mouse); B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1; BMI-1; FLVI2/BMI1; MGC12685; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; RING finger protein 51; RNF51; RNF51Polycomb group RING finger protein 4 BMI1 FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1 BMI1 proto-oncogene, polycomb ring finger polycomb complex protein BMI-1|B lymphoma Mo-MLV insertion region 1 homolog|BMI1 polycomb ring finger oncogene|BMI1 polycomb ring finger proto-oncogene|murine leukemia viral (bmi-1) oncogene homolog|polycomb group RING finger protein 4|polycomb group protein Bmi1|ring finger protein 51

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BMI1, check out the BMI1 Infographic

BMI1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BMI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Polycomb complex protein BMI-1 Bmi1 Antibody

Question

Here is the WB image, lot number and protocol we used for muscle using anti-Bmi1 antibody PA1807. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-01

Question

I see that the anti-Bmi1 antibody PA1807 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-26

Question

Would PA1807 anti-Bmi1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

It shows on the product datasheet, PA1807 anti-Bmi1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-04

Question

Is there a BSA free version of anti-Bmi1 antibody PA1807 available?

V. Wu

Verified customer

Asked: 2019-04-02

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bmi1 antibody PA1807 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-04-02

Question

Will anti-Bmi1 antibody PA1807 work for WB with muscle?

B. Parker

Verified customer

Asked: 2018-08-24

Answer

According to the expression profile of muscle, BMI1 is highly expressed in muscle. So, it is likely that anti-Bmi1 antibody PA1807 will work for WB with muscle.

Boster Scientific Support

Answered: 2018-08-24

Question

Is this PA1807 anti-Bmi1 antibody reactive to the isotypes of BMI1?

G. Zhao

Verified customer

Asked: 2018-02-26

Answer

The immunogen of PA1807 anti-Bmi1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human Bmi1(136-152aa EFFDQNRLDRKVNKDKE), different from the related mouse sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-02-26

Question

I have a question about product PA1807, anti-Bmi1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Anderson

Verified customer

Asked: 2016-02-08

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1807 anti-Bmi1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-02-08

Order DetailsPrice
PA1807

100μg

$370
PA1807-10ug

10μg sample (liquid)

$99
PA1807-Biotin

100 μg Biotin conjugated

$570
PA1807-Cy3

100 μg Cy3 conjugated

$570
PA1807-Dylight488

100 μg Dylight488 conjugated

$570
PA1807-Dylight550

100 μg Dylight550 conjugated

$570
PA1807-Dylight594

100 μg Dylight594 conjugated

$570
PA1807-FITC

100 μg FITC conjugated

$570
PA1807-HRP

100 μg HRP conjugated

$570
PA1807-APC

100 μg APC conjugated

$670
PA1807-PE

100 μg PE conjugated

$670
PA1807-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1807
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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